Binding of Autoantibodies in The Pathogenesis of Small Nerve Fiber - - PowerPoint PPT Presentation

Demonstrating Novel Binding of Autoantibodies in The Pathogenesis of Small Nerve Fiber Neuropathy

Daria Keren, 6th year GM student, University of

Debrecen, Hungary

Guidance by: Efrat Shavit-Stein, Ph.D, Neuroscience Research Lab Tali Drori- Snir, M.D. Neuroimmunology clinic

• Prof. Joab Chapman, M.D, Ph.D, Head of Neurology Department

Amir Dori, M.D, Ph.D, Neuromuscular clinic

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https://www.meresearch.org.uk/our-research/ongoing-studies/role-of-autoantibodies/

7.2.2020

Peripheral Nervous System

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https://www.researchgate.net/figure/The-central-nervous-system-CNS-and-peripheral-nervous-system-PNS-source_fig1_317485174

Autonomic Nervous System

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https://images.app.goo.gl/VGQm17RvXZoUrAjK6

Autonomic Nervous System: Adrenergic Receptors

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https://images.app.goo.gl/BDCuzrFZmZFWjGt29

Large vs. Small Nerve Fibers

Nerve fibers are classified based on their thickness (in diameters) into small fibers and large fibers. This correlates directly to their speed of conduction.

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https://www.researchgate.net/figure/Peripheral-nerve-anatomy-image-courtesy-of-Myoscience_fig1_304365497 Source: https://www.researchgate.net/figure/Classification-of-the-peripheral-nerve-fibers_tbl1_255952315

Peripheral Neuropathy

• One of the most common neurological manifestation, though often

underdiagnosed.

• A manifestation of damages to peripheral nerve fibers, by different

pathologies, that are characterized by impairment to sensation, motoric and autonomic functions.

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https://images.app.goo.gl/GCW86s13BbzoUyWBA

Sorce: https://www.mdedge.com/ccjm/article/175849/neurology/office-approach-small-fiber-neuropathy

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Source:https://bakodx.com/epidermal-nerve-fiber-density-testing-an-overview-2/#post/0

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Accounts for 30-50% of cases

Small Fiber Neuropathy Diagnosis

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https://www.aafp.org/afp/2011/1101/p995.html

Epidermal neurites, Control healthy patient Epidermal neurites, patient with small fiber neuropathy

jamaneurology_oaklander_2019_rv_190004

Source: https://www.frontiersin.org/files/Articles/469534/fneur-10-00970-HTML/image_m/fneur-10-00970-g001.jpg

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Autonomic System in the Skin

Study the Role of Autoantibodies in Small Fiber Neuropathy After Breast Silicone Implantation

• IVIG and plasmapheresis showed improvement.
• Anti β2, α1 and muscarinic antibodies were found in these patients
• May a specific binding can explains the autonomic complaints?
• The major aim is to characterize the specific binding of these autoantibodies

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The Method

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https://images.app.goo.gl/rcx9sx4Fbp2joADv9 https://www.shutterstock.com/de/video/clip-1019518030- white-lab-mouse-albino-close-up-on-background https://lanlab.ee/toode/cryostat-microtome/ https://www.banggood.com/25pcs-Biology-Glass-Prepared-Microscope-Slides-Lab-Specimens-With-Wooden-Box-p- 1124398.html https://images.app.goo.gl/c8TNM8eAxw3qowhD9 https://images.app.goo.gl/Y1Xb xxhZaHVZENye7

Immunofluorescence

Purified IgG from a Patient with High Titer of β2 Receptor Autoantibodies

Blood vessels

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Epidermis

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Sweat Glands are stained Epidermis

Purified IgG from a Patient with High Titer of α1 Receptor Autoantibodies

Sweat Glands are stained

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Epidermis

Purified IgG from a Patient with High Titer of Muscarinic Receptor Autoantibodies

םוכיס

1 . תימונוטואהו תירפירפה םיבצעה תכרעמ תונוש תומקרו םינוש םיכילהת דוקפתל ןתמורתו ףוגב . 2 . לומל םיקד םיבצע יביס ןיב לדבההו היתפוריונ םיבע. 3 .םיקד םיבצע יביס לש היתפוריונ , ללשו הינמיס תועודיה תויגולויטאה. 4 .םיקד םיביס לש היתפוריונ ןוחבא. 5 .תירקיעה ונתרטמ : ידוחייה רשקה תא ןייפאל תולתשומ םישנב םיינומצע םינדגונ לש רועב םיקד םיביס לש היתפוריונמ תולבוסה ןוקיליס.

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