Benefits of Cryopreservation Jonathan A. Cohen, DVM DACLAM - - PowerPoint PPT Presentation

benefits of cryopreservation
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Benefits of Cryopreservation Jonathan A. Cohen, DVM DACLAM - - PowerPoint PPT Presentation

Benefits of Cryopreservation Jonathan A. Cohen, DVM DACLAM Director, Center for Comparative Medicine and Surgery IACUC Clinical Research Forum 2/6/2019 Cryopreservation: Life FAR below zero Embryos, sperm, ovaries can be successfully


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Benefits of Cryopreservation

Jonathan A. Cohen, DVM DACLAM Director, Center for Comparative Medicine and Surgery

IACUC Clinical Research Forum 2/6/2019

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Cryopreservation: Life FAR below zero

 Embryos, sperm, ovaries can be

successfully cryopreserved

 -196o C: Cells remain viable  Used successfully for 30+ years  “Freezes” colonies in time

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Benefits of Cryopreservation: Reduction in Cost

  • Per diem
  • Genotyping
  • Colony

management

  • Overhead
  • Space
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SLIDE 4

Impact of “archival strains”

25% 75%

Strains

Active Inactive

 75% of strains at most

institutions are not actively used

 $6,000-$8,000 to maintain

a colony for a year.

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Benefits of Cryopreservation: Safeguards against genetic drift/Breeding Contamination

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Cryopreservation: Making distribution easier

 Maintains availability  Easier to ship  Reduces

 Maintenance costs  importation time  relocation costs

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SLIDE 7

Benefits of Cryopreservation: Reduction in Recovery Risk

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Cost of Disasters

 $100,000 — the cost to develop a single genetically

engineered mouse strain

 6 to 18 months — the time to develop a genetically

engineered mouse strain

 $6,000 — the minimum yearly cost to maintain a

genetically engineered strain

 Immeasurable — the scientific discoveries that hinge

upon the availability of these mice

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SLIDE 9

How do I participate?

 Visit the Mouse Genetics and Gene Targeting CoRE

Facility website for more information:

 https://icahn.mssm.edu/research/portal/resources/deans-cores/mouse-

genetics-and-gene-targeting  Speak to your CCMS Facility Veterinarian or Facility

Supervisor for guidance