Bacto - Art A Multicomponent Inducible Expression System for - PowerPoint PPT Presentation

“Bacto - Art” A Multicomponent Inducible Expression System for Teaching and Discovery North Carolina Agricultural and Technical State University

North Carolina Agricultural and Technical State University What is Bacto Art? www.ncat.edu

North Carolina Agricultural and Technical State University Who are we Targeting Our Primary target is Middle and High  school students www.ncat.edu

North Carolina Agricultural and Technical State University Project Goals » Create an inducible expression system » Hands-on experience in the fields of molecular and synthetic biology www.ncat.edu

North Carolina Agricultural and Technical State University Construction of Component: 3A Assembly www.ncat.edu

North Carolina Agricultural and Technical State University Construction of Component: 3A Assembly IPTG Xyl www.ncat.edu

North Carolina Agricultural and Technical State University Testing the Component: Function www.ncat.edu 7

North Carolina Agricultural and Technical State University Testing the Component: Function www.ncat.edu 8

North Carolina Agricultural and Technical State University SDS-PAGE Analysis www.ncat.edu 9

North Carolina Agricultural and Technical State University Applications: BactoArt as a Teaching Tool 1. Students “ Paint On ” Inducer www.ncat.edu

North Carolina Agricultural and Technical State University Applications: BactoArt as a Teaching Tool 2. Plate BactoArt Bacteria www.ncat.edu

North Carolina Agricultural and Technical State University Applications: BactoArt as a Teaching Tool 4. Excite FPs using Widefield Illumination 5. Discuss Regulated Gene Expression & Transcription/ Translation www.ncat.edu

North Carolina Agricultural and Technical State University Future Directions  Continue developing the project so that the E. coli strain used can express, at a minimum, four different colors. » Other Fluorescent Proteins will include: • Cyan FP (CFP), Far-Red FPs (FRFP), etc.  Create a system that can make fine detailed art pictures. » Using an inkjet printer, put sugars in a cartridge to print out the desired picture www.ncat.edu

North Carolina Agricultural and Technical State University Future Directions  Create photoswitchable FP Bacto-Art » Moving Pictures through incorporation of photoswitchable FPs, such as Dronpa, which can be turned on and off with different wavelengths of light. UV light Blue Light www.ncat.edu 14

North Carolina Agricultural and Technical State University Acknowledgements  Mentors: Dr. H. Newman & Dr. K. Bernot  The “ Greensboro-Aggies ” iGEM team members.  This work was supported by: » I 3 /iBLEND (NSF #1038160) » RISE (NIH #R25GM076162) » The GSK Foundation. www.ncat.edu 15