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North Carolina Agricultural and Technical State University
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Bacto - Art A Multicomponent Inducible Expression System for - - PowerPoint PPT Presentation
Bacto - Art A Multicomponent Inducible Expression System for - - PowerPoint PPT Presentation
Bacto - Art A Multicomponent Inducible Expression System for Teaching and Discovery North Carolina Agricultural and Technical State University North Carolina Agricultural and Technical State University What is Bacto Art? www.ncat.edu
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North Carolina Agricultural and Technical State University
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Who are we Targeting
- Our Primary target is Middle and High
school students
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North Carolina Agricultural and Technical State University
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Project Goals
» Create an inducible expression system » Hands-on experience in the fields of molecular and
synthetic biology
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North Carolina Agricultural and Technical State University
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Construction of Component: 3A Assembly
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North Carolina Agricultural and Technical State University
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Construction of Component: 3A Assembly
IPTG Xyl
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North Carolina Agricultural and Technical State University
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Testing the Component: Function
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North Carolina Agricultural and Technical State University
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Testing the Component: Function
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North Carolina Agricultural and Technical State University
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SDS-PAGE Analysis
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North Carolina Agricultural and Technical State University
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Applications:
BactoArt as a Teaching Tool
- 1. Students “Paint On” Inducer
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North Carolina Agricultural and Technical State University
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Applications:
BactoArt as a Teaching Tool
- 2. Plate BactoArt Bacteria
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North Carolina Agricultural and Technical State University
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Applications:
BactoArt as a Teaching Tool
- 4. Excite FPs
using Widefield Illumination
- 5. Discuss
Regulated Gene Expression & Transcription/ Translation
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North Carolina Agricultural and Technical State University
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Future Directions
- Continue developing the project so that the E. coli strain
used can express, at a minimum, four different colors. » Other Fluorescent Proteins will include:
- Cyan FP (CFP), Far-Red FPs (FRFP), etc.
- Create a system that can make fine detailed art pictures.
» Using an inkjet printer, put sugars in a cartridge to print
- ut the desired picture
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North Carolina Agricultural and Technical State University
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Future Directions
- Create photoswitchable FP Bacto-Art
» Moving Pictures through incorporation of photoswitchable FPs, such as Dronpa, which can be turned on and off with different wavelengths of light.
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UV light Blue Light
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North Carolina Agricultural and Technical State University
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Acknowledgements
- Mentors: Dr. H. Newman & Dr. K. Bernot
- The “Greensboro-Aggies” iGEM team members.
- This work was supported by:
» I3/iBLEND (NSF #1038160) » RISE (NIH #R25GM076162) » The GSK Foundation.
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