Avena sativa (Oat) Kernel Extract What is AvenaPLex? What is - PowerPoint PPT Presentation

Avena sativa (Oat) Kernel Extract

What is AvenaPLex?

What is AvenaPLex? Extracted from Crude Oat Oil AvenaPLex is a viscous liquid with a deep orange colour that is extracted from crude oat oil using ethanol. Active Lipid Complex (Patent Pending) A 40% polar lipid fraction rich in skin identical and skin equivalent ceramides, sphingolipids and phospholipids. Anhydrous Active An anhydrous active suitable for a wide range of personal care formulations.

AvenaPLex Benefits Barrier Repair Rapidly repairs the skin lipid barrier to reduce TEWL. Ceramide Enrichment Increases the level of essential ceramides in the skin. Improve Skin Elasticity Provides long-lasting hydration and moisturisation for more youthful, radiant skin. Moisturising Up-regulation of Hyaluronan Synthase 3 (hyalyronic acid), a key part of the skin’s natural moisturising factor. Reduce the Signs of Ageing Replenishes skin lipids lost through ageing.

AvenaPLex Lipid Profile

Lipid Profile Summary AvenaPlex is an unique complex containing fatty acids capable of repairing, renewing and protecting the skin lipid barrier. Polar Lipids Percentage Neutral Lipids Percentage Triacylglycerols 37.0 Ceramides 4 Free Fatty Acids 11.0 Phospholipids 15 Sterols 10.0 DGDG / MGDG 10 Diacylglycerol 2.0 Pigmented Material (Unidentified) 10 Total Neutral Lipids 60.0 Total 40

AvenaPLex Polar Lipids

Polar Lipids: Ceramides Approximate Composition of AvenaPLex AvenaPLex Total Ceramides 1.36 Hydroxyceramides Why Are Ceramides Important? Glycosyl Inositol Phosphoryl Ceramides (Proceramide) 1.32 Glucosylceramide 1.32 Total Ceramides 4.00 Skin Barrier Ceramides form part of the skin’s barrier which works to prevent excessive water loss and protect the skin from AvenaPLex contains a significant proportion of the ceramide classes required by the external aggressors. skin: AvenaPLex AvenaPLex Ceramide Class Skin Total incl. Anti-Ageing Identical (%) Isomers (%) By replenishing ceramides lost through ageing, skin is Non-hydroxy-sphingosine [NS] 0.07 0.52 able to fortify itself and retain optimal moisture for a firmer, smoother look. Non-hydroxy-phytosphingosine [NP] 0.13 0.13 Omegahydroxy-6-hydroxy-sphingosine 0.47 0.47 [EOH] Alphahydroxy-sphingosine [AS] 0.05 0.19 Alphahydroxy-phytosphingosine [AP] 0.05 0.05 Total 0.78 1.36

Polar Lipids: Phospholipids Phospholipids Percentage Why Are Phospholipids Important? Phosphatidylcholine (PC) 6.0 Skin Protection Phosphatidylinositol (PI) 3.5 Phosphatidylcholine can improve and protect cells and Pshosphatidylethanolamine (PE) 3.0 membrane damaged by free radicals. Phosphatidic 1.5 acid/Phoshatidyglycerol/cardiolipin Transport Molecules (PG) Phospholipids help to transport important molecules across cell membranes. Lysophosphatidycholine 1.0 Total Phospholipids 15.0%

AvenaPLex Neutral Lipids

Neutral Lipids: Sterols Why Are Sterols Important? Sun Protection Sterol Content Percentage Reduce photosensitivity and provide protection from photo- Beta-Sitosterol 4.0 ageing. Avenasterol 3.5 Collagen Reproduction Other (including cholesterol) 2.0 Promote cell regeneration and accelerate collagen reproduction, a key molecule in skin anti-ageing. Total Sterols 9.5 Anti-Inflammatory Relive skin irritation and reduce redness.

Neutral Lipids: Triacylglycerols Percentag Triacylglycerols e Myristic 14:0 0.07 Pentadecylic 15:0 0.08 Moisturising Palmitic 16:0 4.66 Forms an oily, water-blocking layer on the surface of the skin that slows TEWL to aid moisture retention and softens rough skin. Stearic 18:0 0.55 Arachidic 20:0 0.04 Behenic 22:0 0.04 Total Saturated 5.45

Neutral Lipids: PUFA’s Omega 6 Percentage Why Are Fatty Acids Important? Linoleic 18:2n-6 10.85 Skin Health Eicosadienoic acid 20:2n-6 0.02 Lipid barrier repair – reducing TEWL Total Omega 6 10.87 Omega 3 Percentage Anti-Inflammatory Alpha-Linolenic 18:3n-3 0.35 Omega 3 soothes skin and helps minimise redness associated with dry skin Stearidonic acid 18:4n-3 0.03 conditions such as rosacea or eczema. Total Omega 3 0.37

AvenaPLex Studies

AvenaPLex Studies PROTEIN EXPRESSION PROTEIN CELL VIABILITY EXPRESSION AvenaPLex increases the performance of key gene markers assays and up- regulates hyaluronic acid CERAMIDE INCREASE BARRIER REPAIR OXIDATIVE STABILITY SKIN IDENTICAL LIPIDS SKIN HYDRATION ACNE CONTROL SKIN IDENTICAL LIPIDS

AvenaPLex Studies PROTEIN EXPRESSION PROTEIN EXPRESSION AvenaPLex stimulates the activation of the pathway AvenaPLex increases the performance of key involved in the integrity of epidermal structural gene markers assays and up-regulates hyaluronic proteins and hyaluronic acid. acid

AvenaPLex Studies PROTEIN EXPRESSION PROTEIN EXPRESSION Results Stimulate Proteins Background AvenaPlex upregulates genes including Hyaluronan Synthase 3 (hyaluronic acid) responsible for the expression of proteins involved in barrier function. Comparing AvenaPLex with standard oat oil in a barrier function and hydration gene array model % Increase in Gene Stimulation by AvenaPLex Compared to using a reconstructed human epidermis Oat Oil 300 271 Barrier function measured using a gPCR Array % Increase in gene stimulation 250 200 178,2 169 167 AvenaPLex was applied at 0.005% and standard 150 oat oil was applied at 0.017% 100 50 0 Keratin Differentiation - Keratin Differentiation - Adherens Junction - Extracellular Matrix - trychohyalin repetin occludin hyaluronan synthase 3 Gene Markers

CELL VIABILITY AvenaPLex helps to prevent cells from damaging

AvenaPLex Studies PROTEIN EXPRESSION CELL VIABILITY Results Protect Cells AvenaPLex improves cell viability when used at both 1% and Background 100%; preventing cells from damaging as fast as without treatment Measuring the metabolic activity of the skin cells with application of 1% and 100% AvenaPLex 8 Score Key 7 Clearly altered 1 Study performed using skin explants 6 Fairly clearly from a 59 year old female 2 altered 5 Assessed using immunostaining and image Moderately analysis 4 3 altered Score 3 Slightly altered 4 Very slightly 2 5 altered 1 Fairly good 6 0 Good 7 100% 1% AvenaPLex Control Day Control Day AvenaPLex Day 5 0 5 Day 5

AvenaPLex Studies CELL VIABILITY Oedema Acantholysis (cell detachments) Dying cells Basal Keratinocytes Corneocytes Control at Day 5 Epidermis Basal Layer Papilliary Layer The application of Dermis AvenaPlex induces a regular Stratum Corneum structure, when compared Control at Day 0 to control, with good thick layers of lipid between the corneocytes. The application of AvenaPLex significantly reduces the number of dying basal keratinocytes and cell detachments inducing a much healthier lower epidermis comprised of healthy cells. 1% AvenaPLex at Day 5

AvenaPLex Studies BARRIER REPAIR AvenaPLex rapidly repairs the skin lipid barrier to reduce trans epidermal water loss.

AvenaPLex Studies BARRIER REPAIR Results Barrier Repair on Aged Skin Background AvenaPLex rapidly repairs the skin barrier as demonstrated by the decrease in TEWL upon application. Assessing the effect of 1% AvenaPLex in a base formulation on skin barrier function compared to a placebo Transepidermal Water Loss (1% AvenaPLex) Total Transepidermal Water Volunteers over the age of 40 were 98 included in the study 96 94 92 Trans epidermal water loss of the Loss 90 skin was measured using a 88 Tewameter 86 84 1 hour 2 hours Time AvenaPLex Placebo

AvenaPLex Studies SKIN HYDRATION BARRIER REPAIR Daily application of AvenaPLex significantly increases skin hydration.

AvenaPLex Studies SKIN HYDRATION Background Results To assess the cutaneous hydration of Epidermal Moisture Measurements AvenaPLex, non-invasive capacitance measurements were performed at 5 test AvenaPLex significantly increased skin hydration by 9%, with one participant seeing an improvement of 26%. sites (forehead, temples, cheekbones, jaw lines and chin), with an average taken at Day 0 and Day 28. Hydration Average 62 22 volunteers aged 35 -68 60,9 61 were selected. 60 Corneometer reading 59 All volunteers had oily skin as 58 classified by a dermatologist. 57 56 56 Volunteers answered question about 55 sensory performance after the study. 54 53 Day 0 Day 28

AvenaPLex Studies SKIN HYDRATION Results Consumer Perception Sensory and Performance Scores After 28 Days After 28 days: Non-invasive measurements were performed at 5 test sites (forehead, temples, cheekbones, jaw lines and chin), with an average taken at Day 0 and Day 28. 91% of participants noticed that their skin was more hydrated. 100% 91% 86% 82% 82% 82% 90% 77% 77% 59% 86% of participants agreed that their 45% 80% 50% 55% 37% skin felt smoother and softer. 50% 36% 70% 60% 50% 82% agreed it improved skin texture. 40% 30% 45% 20% 41% 41% 32% 32% 27% 27% 77% agreed that they had more 10% radiant and supple skin. 0% Hydrating effect More hydrated More supple Smoother and More radiant Skin texture is Global after application skin skin softer skin skin improved performance Agree Strongly agree

AvenaPLex Studies IMPROVING LIPID BARRIER AvenaPLex is a good source of lipids to supplement the skin’s natural lipid profile