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Autoimmune inflammation and the Autoimmune inflammation and the - - PowerPoint PPT Presentation
Autoimmune inflammation and the Autoimmune inflammation and the - - PowerPoint PPT Presentation
Autoimmune inflammation and the Autoimmune inflammation and the human microbiome human microbiome Trevor G. Marshall, Trevor G. Marshall, Trevor G. Marshall, Trevor G. Marshall, Computational microscope Computational microscope
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Computational microscope Computational microscope Computational microscope Computational microscope Computational microscope Computational microscope Computational microscope Computational microscope Computational microscope Computational microscope Computational microscope Computational microscope Computational microscope Computational microscope Computational microscope Computational microscope
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FAB regions of IgG Extracellular region of TSH Receptor Autoantibody was isolated from patient with Graves disease.
Autodock Tools Autodock Tools Molecular Graphics Lab
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FAB regions of IgG Extracellular region of TSH Receptor TSH Receptor
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EXAMPLE - EBV “We immortalized human B cell lines “We immortalized human B cell lines from normal individuals and from patients with malignant or benign dysglobulinemia with Epstein-Barr virus and examined, after cloning, the autoantibody reactivities of the immunoglobulins secreted by these two supernatants were two supernatants were analyzed by enzyme-immunoassay on a panel of 13 self and non-self antigens: trinitrobenzenesulfonic acid (TNP), glutamine, L-alanine, L- tyrosine (GAT), actin, myosin, tubulin, albumin, renin, spectrin, transferrin, thyroglobulin, myoglobin, peroxidase .. .. .. .. thyroglobulin, myoglobin, peroxidase .. .. .. .. The vast majority of these clones The vast majority of these clones The vast majority of these clones The vast majority of these clones EXAMPLE – Tetanus Toxoid "Human-human hybridomas produced Barr virus "Human-human hybridomas produced from lymphocytes of normal individuals yielded seven clones producing monoclonal antibody reacting with tetanus toxoid.
- f these antibodies cross
- f these antibodies cross
- f these antibodies cross
- f these antibodies cross
with diphtheria toxoid. with diphtheria toxoid. with diphtheria toxoid. with diphtheria toxoid. and two others also reacted with and two others also reacted with and two others also reacted with and two others also reacted with and two others also reacted with and two others also reacted with and two others also reacted with and two others also reacted with cardiolipin and two with other cardiolipin and two with other cardiolipin and two with other cardiolipin and two with other phospholipids phospholipids phospholipids phospholipids" " " "
(M Sutjita, A Hohmann, R Bradley: Polyspecific antibodies to diphtheria and
- phospholipids. Clin Exp Imm
- phospholipids. Clin Exp Imm
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antibodies recognizing EBNA1 400–413 , possibly through a molecular mimicry mechanism. Indeed, the fact that peptides from different pathogens can be cross epitopes supports the hypothesis that EBV and MAP might trigger autoimmunity through a common target”
(Mameli G, et al: Epstein-Barr virus and Mycobacterium avium subsp. paratuberculosis peptides are cross recognized by anti-myelin basic protein antibodies in multiple sclerosis patients. J Neuroimmunol. Mar
413 and MAP 121–132 cross- , possibly through a molecular mimicry mechanism. Indeed, the fact that peptides from different pathogens can be cross-recognized by antibodies targeting epitopes supports the hypothesis that EBV and MAP might trigger autoimmunity through a common target”
Barr virus and Mycobacterium avium subsp. paratuberculosis peptides are cross myelin basic protein antibodies in multiple sclerosis patients. J Neuroimmunol. Mar 6)
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About 110- (not all are functional)
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**Gazillion**
In Mouse, 17,431 unique 17,431 unique antibodies to date
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began development of a mass screening assay began development of a mass screening assay to try and determine which of the victims were most likely to develop disease from that
- exposure. He has developed the ‘ELI
Viscero’ tests for Natural Autoantibodies began development of a mass screening assay began development of a mass screening assay to try and determine which of the victims were most likely to develop disease from that
- exposure. He has developed the ‘ELI
Natural Autoantibodies
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Profiles of serum Natural auto-Abs
Marker of Vasculitis
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CD19+
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Antibodies are far more complex and numerous than we ever dreamed was possible dreamed was possible Human antibodies are mostly polyspecific, ‘autoantibodies’ is a misnomer indicating their potential for ‘collateral damage’ The most likely trigger for persistent antibody production in The most likely trigger for persistent antibody production in humans are the microbes comprising the Human Microbiome The failure of innate immunity will lead to energetic stimulation of antibody, and hence autoantibody, production Stimulation of the innate innate immune system against the Antibodies are far more complex and numerous than we ever dreamed was possible dreamed was possible Human antibodies are mostly polyspecific, ‘autoantibodies’ is a misnomer indicating their potential for ‘collateral damage’ The most likely trigger for persistent antibody production in The most likely trigger for persistent antibody production in humans are the microbes comprising the Human Microbiome The failure of innate immunity will lead to energetic stimulation of antibody, and hence autoantibody, production immune system against the
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Question Time Question Time Question Time Question Time Question Time Question Time Question Time Question Time
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Luc Montagnier, Nobel Prize in Physiology or Medicine in 2008 “Digital transmission of bacterial DNA in living cells” Giuseppe Vitiello, Professor of Theoretical Physics, University of Salerno “A quantum field approach to living matter: why fields? why quantum?” “A quantum field approach to living matter: why fields? why quantum?” Luc Montagnier, Nobel Prize in Physiology or Medicine in 2008 “Digital transmission of bacterial DNA in living cells” Giuseppe Vitiello, Professor of Theoretical Physics, University of Salerno “A quantum field approach to living matter: why fields? why quantum?” “A quantum field approach to living matter: why fields? why quantum?”
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The YouTube videos I took can be viewed at these addresses: Luc Montagnier: https://www.youtube.com/watch?v=mYcNXjdPXGU Luc Montagnier: https://www.youtube.com/watch?v=mYcNXjdPXGU Prof Marc Henry: https://www.youtube.com/watch?v=V0Qrn Prof Carlo Ventura: https://www.youtube.com/watch?v=O3lvFJgXScM Prof Guiseppe Vitiello: https://www.youtube.com/watch?v=s4s fEYmZFo The YouTube videos I took can be viewed at these addresses: Luc Montagnier: https://www.youtube.com/watch?v=mYcNXjdPXGU Luc Montagnier: https://www.youtube.com/watch?v=mYcNXjdPXGU Prof Marc Henry: https://www.youtube.com/watch?v=V0Qrn Prof Carlo Ventura: https://www.youtube.com/watch?v=O3lvFJgXScM Prof Guiseppe Vitiello: https://www.youtube.com/watch?v=s4s fEYmZFo
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Known to be attacked by elements of Microbiome (eg. M. leprae
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