ATP Hygiene Monitoring What is it? A test method to verify good - - PowerPoint PPT Presentation

MASTER CLASS

ATP HYGIENE MONITORING & RAPID TESTS FOR

COLIFORM/E.COLI/TOTAL COUNT 9TH FOOD SAFETY AND & QUALITY SUMMIT NEW DELHI 2&3 DECEMBER 2014

ATP Hygiene Monitoring

What is it?

• A test method to verify good cleaning wherever you want it,

whenever you want it

• Portable, easy and ready for use
• Tests can be done at line, giving instant feedback on status of

cleanliness Ideal for industries with a need for rapid cleaning control and who want to improve their hygiene and quality standards Key advantage is speed and ease-of-use

PURPOSE OF CLEANING

• Primary purpose is to remove organic soiling i.e.

– Environmental Cleaning (Stage 1) – Also reduced microbial bioburden – Ideal test is a direct, instant test for organic residue e.g. ATP

• Only after good cleaning can disinfectants can be applied with

confidence

– Disinfection Cleaning ( Stage 2)

• Hygiene monitoring is an assessment of the cleaning regime
• ver time i.e. supervise and inspect

– Are standards being maintained? Can we improve it?

PROVEN TECHNOLOGY

• ATP tests have been around since late 80’s and are approved

as rapid cleaning verification test

• British Retail Consortium have developed a global standard

for food safety and quality, involving ATP testing. Edition 6, page 40 advises the use of ATP for cleaning verification.

• Compliant with HACCP
• Value for money

ATP HYGIENE MONITORING SYSTEM

Two part system composed of

• 1. Hardware = Detection instrument
• supported by data analysis software
• 2. Swab device for sample collection and testing

Designed for: Simplicity ( easy of use) Convenience Robustness Affordability Sustainability Multi-functional

COMPARISON WITH OTHER METHODS

Visual Microbiology ATP Fast?

++

• ++

Sensitive?

• ++

++ Detection of product residues? +

• ++

Simple? ++

• ++

What is clean?

ATP stands for Adenosine-Tri-Phosphate, which is the energy source of all living

• rganisms and food.

If ATP is detected on a surface, it means it has not been cleaned properly. Sources of ATP: Food Microorganisms People

WHAT IS ATP?

Detection of ATP by bioluminescence reaction ATP reacts with luciferine/luciferase enzyme complex to generate a light reaction, which is measured in a sensitive and dedicated luminometer The ATP test is the most common used rapid test in the world to determine cleaning efficiency.

MEASUREMENT PRINCIPLE

very poor ATP (expressed in RLU) low Cleaning result high excellent

ATP VS CLEANING RESULT

The more food residues or microorganisms, the more ATP is detected, the higher the light output.

Aquasnap

• For water samples (like rinse water after Cleaning in Place, CIP)
• Dip Aquasnap into the water
• Immediate result

Ultrasnap & Supersnap

• For surface hygiene monitoring
• For flat as well as for irregular surfaces
• Immediate result

TWO FORMATS

Swab

2

Snap

3

Squeeze Read

4

PROCEDURE

Interpretation is done by pass/fail limits, which are already pre-set in the instrument. For the most common food contact surface (stainless steel), these pass/fail limits are: Pass: <10 RLU (√) Caution: 11-30 RLU (!) Fail: >30 RLU (X)

SIMPLE INTERPRETATION

BENEFITS OF ATP SYSTEM

• Simple and easy to use by anyone , anywhere, any time
• Providing instant objective quantifiable data

– Permits immediate corrective action

• Identify hot spots and potential risk areas
• Optimises cleaning processes
• Demonstrates and reinforces Best Practice
• Provide real data for Quality monitoring ,

– evidence of due diligence, – value for money

• Supports training and awareness
• Enables team building through more effective direct communication
• Restores pride, ownership and commitment of cleaning staff
• Encourages behaviour change

Clean it …… Prove It!

WELL KNOWN HYGIENA USERS

MICROSNAP RAPID DETECTION OF COLIFORMS/E.COLI & TOTAL COUNT

These are applications that require sample preparation and handling and therefore cannot be done at line. But nevertheless very simple and reliable.

• Microsnap Coliform/E.coli – a very rapid and low cost

screening test for Coliforms and E.coli. Total test time 6-8 hours.

• MicroSnap Total – a rapid low cost screening test for total

viable counts. Total test time 7 hours.

MICROSNAP RANGE

MICROSNAP COLIFORM/E.COLI SPECIFIC DETECTION

Sensitive luminometer Microbial detection Specific enzyme detection AOAC approved

SAMPLE TYPES

• Surface swabs
• Raw material and finished product
• Solids , liquids and filterable products
• Food & beverage; dairy; cosmetics & pharma

and water

TEST PROCEDURE

After incubation, transfer 100µl from the enrichment device (A) and transfer to the detection device (B). Interpret RLU results Read RLU in luminometer

100µl

Activate detection device (B) with a snap and a squeeze. Incubate for 10 minutes at 37°C

INCUBATION TIME

Incubation Time (37C) (h) Lowest Inoculum Detected with 95% certainty 1 <500000 2 <100000 3 <10000 4 <1000 5 <100 6 <10 7 <5 8 1

VIDEO

MICROSNAP TOTAL

• Sensitive luminometer
• Two-step procedure
• 7 Hours incubation (Step 1), followed by

immediate measurement (Step 2)

• AOAC approval pending

SAMPLE TYPES

• Surface swabs
• Raw material and finished product
• Solid and liquid samples
• Food & beverage; dairy; cosmetics & pharma

and water

TEST PROCEDURE

After incubation, transfer 100µl from the enrichment device (A) and transfer to the detection device (B). Interpret RLU results

100µl

Activate detection device (B) with a snap and a squeeze. Read result in luminometer

INTERPRETATION OF RESULTS

Correlation between CFU/ mL and average RLU After 7 hour Incubation at 30°C CFU per ml/100cm2 EnSURE RLU < 100 < 10 < 200 < 20 < 300 < 30 < 500 < 50 < 1,000 < 100 < 10,000 < 1,000 < 100,000 < 9,999

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THANK YOU