VI Magnetobiology Cell integration with nickel nanowires - - PowerPoint PPT Presentation

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VI Magnetobiology Cell integration with nickel nanowires - - PowerPoint PPT Presentation

Dec 06, 2022 163 likes •412 views

VI Magnetobiology Cell integration with nickel nanowires Toxicity study Ac field effect on pre-osteoblasts In vitro cell stimulation with strong pulsed fields Planned work Staff, Publications Adriele Prina-Mello

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VI Magnetobiology

  • Cell integration with nickel nanowires
  • Toxicity study
  • Ac field effect on pre-osteoblasts
  • In vitro cell stimulation with strong pulsed fields
  • Planned work
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Staff, Publications

  • Adriele Prina-Mello Senior postdoc 2006-
  • Fiona Byrne Postgrad 2006-
  • Darragh Crotty Postgrad 2005-
  • Nigel Carrol Experimental Officer
  • Collaborations: Yuri Volkov (TCD, Clinical Medicine)
  • Ken Donaldson, Craig Poland (U. Edinburgh)

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  • Internalization of ferromagnetic nanowires by different living cells, A.

Prina-Mello, Zhu Diao and J. M. D. Coey, Journal of Nanobiotechnology 4:9 1-11 (2006)

  • High content analysis of the biocompatibility of nickel nanowires. Fiona

Byrne, A. Prina-Mello, A. Whelan, B.M. Mohamed, A. davies, Y. Gunko, J.M.D. Coey, Yuri Volkov, Journal of Magnetism and Magnetic Materials, (in press) (2009)

  • Magnetic-fluorescent nanocomposites for biomedical multitasking S A.

Corr, A. O’Byrne, Y. K. Gun’ko, S. Ghosh, D. F. Brougham, S. Mitchell, Y Volkov and A Prina-Mello, Chemical Communications, 4474 - 6 (2006)

  • The

evolution

  • f

chemotaxis assays from static models to physiologically relevant platforms S. Toetsch, P. Olwell, A. Prina-Mello and Y. Volkov, Integrative Biology, (in press)(2009)

  • In vivo EPR for dosimetry, H.M. Swartz, G. Burke and M. Coey,

Radiation Measurements 42 SI 1075-1084 (2007)

Publications

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MANSE Proposal – Magnetobiology

  • Investigate the influence of static uniform magnetic fields

and field gradients on cellular growth and morphology.

  • Manipulate and stimulate cells in novel ways, especially

using ferromagnetic nanowires and pulsed magnetic fields.

MANSE Magnetobiology proposal concept: Living Cells + Magnetic materials + External Field = Manipulation, Stimulation and Sensing

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Cell-NW interaction and Orientation

MSCs with internalized nickel nanowires

Angular distributions of MSC+NW morphology in 0 field (left), and after alignment in a 100 mT applied field for 18 h (right) Angular distributions of Ni NW orientation in MSCs in zero field (left). After alignment in a 100 mT applied field for 18 h (right).

Cell-NW separation and manipulation

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Are the nanowires really internalised?

MC3T3-E1 osteoblasts showing internalized nickel nanowire: SEM micrograph Confocal and Z stack confocal micrographs

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HCS quantitative time response on 20 μm Ni NW

3 hr 6 hr 24 hr 72 hr

NW Concentration NW Concentration

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AC field effect on pre-osteoblasts ?

Background

Literature suggests that low frequency magnetic field can affect osteoblast proliferation, differentiation and morphology

(Heermeier et al., Bioelectromagnetics, (1998),19:222-231).

Pulsed electromagnetic fields have been used clinically and are thought to enhance tissue regeneration (Shupak et al., Radio Science Bulletin, (2003), 307:9-34).

Objective

Understand if there is a scientific basis behind the fears concerning the carcinogenicity of 50/60 Hz AC electromagnetic fields, as suggested by some previous studies (Fedrowitz et al., Cancer Res, 62 (2002) 1356-1363; Zhu et al., Am. J. Epidemiol.,

158 (2003) 798-806).

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In vitro cell stimulation with pulsed fields

Transcranial Magnetic Stimulation (TMS)

Important experimental tool in Neuroscience used in clinically for treatment and diagnosis of neurological disorders such as multiple sclerosis and Parkinson’s disease

Principle of work in neurons

Strong magnetic pulse produced by a flat coil induces a potential difference across the neurons membrane which triggers the neurons firing Objectives

To extend the first demonstration of in vitro magnetic stimulation of neurons reported recently by Rotem et al. (2008) To understand the physical and biochemical mechanisms involved in vitro

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Conclusions, future plans

  • This work is developing valuable interdisciplinary relations within TCD

and beyond

  • Nanowires have potential for cell labelling and manipulation
  • Demonstration of toxicity based on geometrical size and shape, requiring

further examination

  • Parameters associated with bone cell development and carcinogenesis

not affected by weak AC fields

  • In-vitro pulsed-field stimulation is a new and currently understudied area,

which will be developed

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