Understanding and Managing Pecan Bacterial Leaf Scorch Southeastern - - PowerPoint PPT Presentation

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Understanding and Managing Pecan Bacterial Leaf Scorch Southeastern - - PowerPoint PPT Presentation

Understanding and Managing Pecan Bacterial Leaf Scorch Southeastern Pecan Growers Association Convention Destin, Florida February 26-27, 2016 Rebecca A. Melanson , Extension Plant Pathologist Central MS Research and Extension Center


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Understanding and Managing Pecan Bacterial Leaf Scorch

Southeastern Pecan Growers Association Convention Destin, Florida • February 26-27, 2016 Rebecca A. Melanson, Extension Plant Pathologist Central MS Research and Extension Center • Raymond, MS

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A Bit of History

  • Disease first recognized in 1972
  • Identified as a bacterial disease

and named pecan bacterial leaf scorch (PBLS) in 2000

  • Xylella fastidiosa subsp.

multiplex (2012)

  • Lives in the water-conducting

tissues (xylem) of the plant PBLS in ‘Cape Fear’ pecan.

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PBLS Symptoms

Late-stage PBLS symptoms. ‘Early’-stage PBLS symptoms.

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PBLS Symptoms

PBLS in a mature tree. PBLS in a young grafted tree.

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Moreland Navaho Nacono *Oconee *Pawnee *Rome Schley

Cultivar Susceptibility

*Barton Caddo *Cape Fear Candy Cherokee *Cheyenne Desirable Elliott Farley Forkert Jackson Kiowa Mahan Melrose Shoshoni Stuart *Sumner Woodman Natives/seedlings

NO RESISTANT CULTIVARS HAVE BEEN IDENTIFIED.

Reference: Sanderlin, 2005, Plant Disease 89:446-449.

*Highly susceptible cultivars.

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SLIDE 6

Time Period % Leaflet Defoliation % Reduced Kernel Weight % Reduced Nut Weight Year 1 67 19 29 Year 2 66 16 32 Year 3 41 14 20 Average 58 16.3 27 Age of Trees % Less Diameter % Less Height 4 Years Old 43 40 9 Years Old 52 N/A

Effect of PBLS on Yield and Tree Growth

Yield Tree Growth

References: Sanderlin and Heyderich-Alger, 2003, Plant Disease 87(3):259-262.; Sanderlin, unpublished

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Graft Transmission in Pecan

PBLS in new growth from infected scion.

  • Infected scions

–21% transmission rate

  • Infected rootstocks

–85% transmission rate

References: Sanderlin and Melanson, 2008, Plant Disease 92:1124-1126.; Sanderlin and Melanson, 2006, HortScience 41(6):1455-1456.

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Prepared scions for storage. Submerged scions in hot water. Grafted to non-infected rootstock. Identified PBLS-infected limbs.

Hot-water Treatment of Scions

Bundled scions with a weight.

Treatment

  • 115ºF water for 30 min
  • Room temperature

water for 1-2 min Completely submerge!

The link to the publication “Procedure for Hot Water Treatment of Pecan Scion Wood” can be found at the end of this presentation.

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Two-Year Totals # of Scions Tested % Graft Success # of Trees with PBLS Symptoms # of X. fastidiosa- Infected Trees Hot-water Treated Scions

165 84.8 2 1

Non-treated Scions

105 84.7 18 19 0.7 21.3

20 40 60 80 100

Hot-water-treated scions Non-treated scions % Graft-transmission of X. fastidiosa

At a 21% graft-transmission rate, 29 of the 140 hot- water-treated scions should have been infected. **97% EFFECTIVE**

Hot-water Treatment of Scions

Reference: Sanderlin and Melanson, 2008, Plant Disease 92:1124-1126.

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PBLS Spread in a Mature Orchard

  • Orchard location:

LSU AgCenter Pecan Research-Extension Station, Shreveport, Louisiana

  • Planting date:

1986 or 1987

  • Composition:

– 3 cultivars and non- grafted trees – 5 rootstocks Year Number of Diseased Trees/Number of Trees in Orchard (% Infected Trees) 2001 5/410 (1.2%) 2002 12/410 (2.9%) 2003 17/409 (4.2%) 2004 33/390 (8.5%) 2005 63/390 (16.2%) 2006 91/390 (23.3%)

An increase in the number of PBLS-infected trees was

  • bserved in all cultivars and in most non-grafted trees.

However, the greatest increase in the number of infected trees occurred in cultivar Cape Fear, the cultivar in the

  • rchard with the highest susceptibility.
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Insect Transmission in Pecan

Fed captured insects on infected pecan tissue and then caged them

  • nto Cape Fear pecan seedlings.

Collected potential vectors – xylem-feeding insects (spittlebugs and sharpshooters).

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  • Percent transmission varied from 2% – 100% depending on the insect species

Insect Transmission in Pecan

Lateral-lined sharpshooter Johnsongrass sharpshooter Diamond-backed spittlebug Glassy-winged sharpshooter Pecan spittlebug (adult) Photos by: S. Gil Photo by: R. Melanson 2 – 11.4% 100% 19.6% 16.7% 4%

Reference: Sanderlin and Melanson, 2010, Plant Disease 94(4):465-470.

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  • Dr. R. S. Sanderlin at the LSU AgCenter

Pecan Research-Extension Station in Shreveport, LA, has monitored five pecan

  • rchards in Louisiana for the presence of

known and potential vectors of Xylella fastidiosa using yellow sticky traps. Data indicated that the greatest number of known and potential vectors were present in

  • rchards from May through August.

Monitoring Pecan Orchards for Vectors*

Orchard locations:

*Known and potential vectors

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Subspecies of Xylella fastidiosa

  • Subspecies fastidiosa (2004)

– Almond, grapevine

  • Subspecies multiplex (2004)

– Almond, peach, oak, pecan

  • Subspecies pauca* (2004)

– Citrus, coffee

  • Subspecies sandyi (2005)

– Oleander

  • Subspecies tashke (2009)

– Chitalpa

Pierce’s disease

  • A. H. Purcell, Univ. CA - Berkeley, Bugwood.org

Citrus variegated chlorosis*

  • A. H. Purcell, Univ. CA, Bugwood.org

Bacterial leaf scorch in pin oak

  • J. Hartman, Univ. KY, Bugwood.org

Bacterial leaf scorch in sycamore

  • T. D. Leininger, USDA Forest Service, Bugwood.org

*Not currently present in the U.S.

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  • FOCUSED ON PREVENTION!
  • Plant non-infected trees
  • Use non-infected rootstock
  • Collect scion wood from non-infected trees
  • Hot-water treatment of scions
  • Monitor vectors in orchards and apply insecticides

during peak trapping periods or at first appearance (?)

  • Avoid planting/remove plants near orchards that

attract vectors OR manage vectors on those plants (?)

  • Branch or tree removal (?)

Management of PBLS

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Thank you for your attention!

  • Rebecca A. Melanson

Extension Plant Pathologist Central Mississippi Research and Extension Center Mississippi State University Phone: (601) 857-2284 Email: rebecca.melanson@msstate.edu

  • R. S. Sanderlin

Research/Extension Plant Pathologist LSU AgCenter Pecan Research-Extension Station Louisiana State University Email: RSanderlin@agcenter.lsu.edu

Photo by MSU Extension

The research discussed in this presentation was conducted by

  • R. A. Melanson and R. S. Sanderlin at the LSU AgCenter

Pecan Research-Extension Station or at Louisiana State

  • University. Unless otherwise specified, photographs were taken

by R. A. Melanson or R. S. Sanderlin.

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  • Articles:

– Pecan Bacterial Leaf Scorch Cultivar Susceptibility and Pathogen Transmission – Pecan Bacterial Leaf Scorch: A New Look at an Old Problem – Procedure for Hot Water Treatment of Pecan Scion Wood

  • Additional photos of PBLS (search ‘Xylella fastidiosa and pecan’)

– www.ipmimages.org/

Additional Resources

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  • Melanson, R. A. and Sanderlin, R.S. 2015. Hot-water treatment of pecan scions as a means of phytosanitation to reduce the potential introduction of

Xylella fastidiosa, the causal agent of pecan bacterial leaf scorch, into orchards and new geographic regions. Acta Horticulturae 1070:201-209.

  • Melanson, R. A., Sanderlin, R. S., McTaggart, A. R. and Ham, J. H. 2012. A systematic study reveals that Xylella fastidiosa strains from pecan are

part of X. fastidiosa subsp. multiplex. Plant Disease 96:1123-1134.

  • Li, B., Sanderlin, R. S., Melanson, R. A., and Yu, Q. 2011. Spatial analysis of a plant disease in a non-uniform crop: a Monte Carlo approach. Journal
  • f Applied Statistics 38(1):175-182.
  • Sanderlin, R. S. and Melanson, R. A. 2010. Insect transmission of Xylella fastidiosa to pecan. Plant Disease 94(4):465-470.
  • Sanderlin, R. S., Li, B., Melanson, R. A., and Gil, S. 2009. Spread of Xylella fastidiosa in a pecan orchard and presence of potential vectors in
  • rchards. Phytopathology 99:S114. (abstract)
  • Sanderlin, R. S. and Melanson, R. A. 2008. Reduction of Xylella fastidiosa transmission through pecan scion wood by hot-water treatment. Plant

Disease 92:1124-1126.

  • Sanderlin, R. S. and Melanson, R. A. 2006. Transmission of Xylella fastidiosa through pecan rootstock. HortScience 41(6):1455-1456.
  • Sanderlin, R. S. 2005. Cultivar and seedling susceptibility to pecan bacterial leaf scorch caused by Xylella fastidiosa and graft transmission of the
  • pathogen. Plant Disease 89:446-449.
  • Sanderlin, R. S. and Heyderich-Alger, K. I. 2003. Effects of pecan bacterial leaf scorch on growth and yield components of cultivar Cape Fear. Plant

Disease 87(3):259-262.

  • Sanderlin, R. S. and Heyderich-Alger, K. I. 2000. Evidence that Xylella fastidiosa can cause leaf scorch disease of pecan. Plant Disease 84 (12):

1282-1286.

  • Sanderlin, R. S. 1998. Evidence that Xylella fastidiosa is associated with pecan fungal leaf scorch. Plant Disease 82:264.

PBLS Research Publications