The Power of Small The Bio-NEMS drill *Patent pending Resonator - - PowerPoint PPT Presentation

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The Power of Small The Bio-NEMS drill *Patent pending Resonator - - PowerPoint PPT Presentation

Jan 25, 2023 147 likes •611 views

The Power of Small The Bio-NEMS drill *Patent pending Resonator Image obtained from MEMS & Nanotechnology Exchange. <https://www.mems-exchange.org/MEMS/what-is.html> *Patent pending DNA Coiling *Patent pending Biological

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The Power of Small

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The Bio-NEMS drill

*Patent pending

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Resonator

*Patent pending Image obtained from MEMS & Nanotechnology Exchange. <https://www.mems-exchange.org/MEMS/what-is.html>

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DNA Coiling

*Patent pending

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Biological Resonator?

*Patent pending

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IDEA: Mimic the chromatin’s nucleosome

*Patent pending

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Survey

What is a Microelectromechanical System (MEMS)? NEMS

28.77 71.23

Know what a MEMS is Do NOT know what a MEMs is

*Patent pending Fedder, G.K. ; Carnegie Mellon Univ., Pittsburgh ; Howe, R.T. ; Tsu-Jae King Liu ; Quevy, E.P.

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Movie Projection

Disseminate Nanotechnology and its contribution to Synthetic Biology

*Patent pending

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Some assumptions were made:

  • All NPs are monocrystalline and equal

(Fe3O4, r=11nm and Fe3O4@SiO2, r= 21nm)

  • The DNA coiled around acts as a rigid body.
  • The force to unbind DNA from the protein is

about 6.3 pN.

6.3 pN

*Patent pending

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Some assumptions were made:

  • All NPs are monocrystalline and equal

(Fe3O4, r=11nm and Fe3O4@SiO2, r= 21nm)

  • The DNA coiled around acts as a rigid body.
  • The force to unbind DNA from the protein is

about 6.3 pN.

  • DNA mass is insignificant compared to the

NP’s.

  • All drags are the same
  • Homogeneous medium
  • The system can be reduced to a single mass

and drag

6.3 pN

*Patent pending

Bio – NEMS Drill

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Lorentz force equation Drag force equation The amount of Bohr magnetons per unit cell Number of paired electrons Charge Total magnetic force Total force Amount of Nanoparticles

*Patent pending

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*Patent pending

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The Bio-NEMS drill

  • Individual Components:
  • Magnetic Nanoparticles
  • Peptide
  • Cross-linking agent
  • DNA strand

*Patent pending

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Nanoparticle Synthesis

  • Co-precipitation

Method

  • More tan 50 trials
  • Functionalization
  • Biocompatible

*Patent pending

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Magnetite

  • Superparamagnetic
  • 22 nm monodisperse

*Patent pending

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Peptide Assembly

  • Trial 1: SASP
  • Trial 2: HU
  • DNA – binding
  • Electrophoresis

*Patent pending

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The Bio-NEMS drill

*Patent pending

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Law Initiative

Difficulties with the importation of reactants and biological agents!

A law initiative came to be as a collaboration with the Tec Monterrey team.

*Patent pending

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Is this real?

*Patent pending

Characterization

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UV Spectroscopy 260 nm Blue: Np + DNA Green: Nanoparticles

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Use of plasmids for characterization

  • f the device

*Patent pending

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Bio-NEMS Drill as a cloning vector

Improve genetic transformation

*Patent pending

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*Patent pending

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*Patent pending

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And the Brainstorm came…

*Patent pending

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*Patent pending

The Test

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Does it Work?

*Patent pending

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Results

BBa_K737051 Used as control and DNA in our system for a traditional transformation and a Electroporation Method 60 to 80 Colonies in our Control against 80 to 100 in the enhanced electroporation

*Patent pending

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The Joy of Sharing

*Patent pending

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Achievements

Research fund for students’ projects!!! Deeper understanding

  • f Synthetic

Biology!!!

*Patent pending

Low Budget project with great results!!!

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*Patent pending

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What’s next?

*Patent pending

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*Patent pending

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*Patent pending

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IT IS THE REAL DRILL!!

*Patent pending

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iGEM Berlin

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*Patent pending

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*Patent pending

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Marvellous doesn’t always come from complexity