Table of Contents Introduction video Team introduction Background - - PowerPoint PPT Presentation

I N V E S T I GAT I N G T H E M U LT I D R U G R E S I S TA N C E O F M RSA A N D T H E G E N E S I N VO LV E D

Table of Contents

— Introduction video — Team introduction — Background — Safety — Equipment — Process — Results — Conclusions — Further research — Human practice — Sponsors

Introduction Video

Team Introduction

— South Korea — Elan Vital — Multi-school biology club — Experimented in the Gachon medical university

Team Members

Ye Eun Lee Ji Hoon Kim Young Chan Kim Soo Jung Lee Ji Yong Hong

Yae Sung Mun

— Team coach — Graduate in Gachon university

majoring in bio-nano technology

— Plays the flute

You Jin Hwang

— Team instructor — Professor in Gachon university — Loves to play soccer and

badminton

MRSA

— Methicillin Resistant Staphylococcus aureus — Resistance to beta-lactam antibiotics — Causes a variety of diseases including

life-threating ones

— Nosocomial infections (Infections in hospitals)

What We Did

— Multidrug resistance of MRSA and the genes

involved

— Comparing DNA of bacteria that has drug

resistance: like finding a common denominator

— MRSA with drug resistance & transformed

• E. coli with drug resistance

Transformation

— Insert foreign DNA in cell — DNA codes proteins — The cell will produce the

protein that the DNA codes for

Safety

— WHO Biosafety Levels — E. coli WHO BSL 2 — MRSA WHO BSL 3 — Gachon University of Medicine safety

regulations

Equipment

— We used lab equipment from the Gachon university o

f medicine lab

Process

— MRSA + antibiotics — Transform E. coli with surviving MRSA DNA — PCR on the DNA of the surviving bacteria — Gel electrophoresis

MRSA Antibiotic Resistance

— Grow MRSA sample with antibiotics — In the project, we used MRSA from samples #7 and#8, and

the antibiotics ampicillin, gentamycin, kanamycin, and tetracycline

— LB plates of 1/1000 concentration of stock solution — Stock solutions of concentrations 100mg/μl for ampicillin,

50mg/μl for gentamycin, 10mg/μl for kanamycin, and 5mg/μl for tetracycline.

MRSA Antibiotic Resistance Results

• E. Coli Antibiotic Resistance

— Competent E. coli — Control group for transformation — Competent E. coli are designed to be easily

transformed

• E. Coli Antibiotic Resistance Results

Transformation

— Transform E. coli with DNA from MRSA — Extract DNA from MRSA though miniprep — In this case, we will transform E. coli (which was

confirmed to not have antibiotic resistance) with MRSA that developed drug resistance

— We want E. coli to develop drug resistance

Transformation Results

PCR

— Polymerase chain

reaction

— Amplifies DNA — Makes it easier to

runtests such as gel electrophoresis

PCR Results (Amplification)

Gel Electrophoresis

— Gel electrophoresis is

used to analyze DN A

— Good for

comparisons

Gel Electrophoresis

— We can use gel electrophoresis to compare

the DNA of the MRSA and E. coli with antibiotic resistance

— We can find the section of DNA that is shared

in both types of bacteria by comparing gel electrophoresis

Gel Electrophoresis Results

Conclusion

— Successfully transformed E. coli to be drug

resistant

— Similar bands in gel electrophoresis that could code

for multidrug resistance

— We would like to sequence the similar bands

Future Research

— Sequence DNA sections from gel electrophoresis — Understanding the genes of drug resistance

• f MRSA

— Finding alternate antibiotics — Finding chemicals to counteract drug resistance

Human Practices

— To raise public awareness on MRSA, we handedout

pamphlets, hanged up posters, and gave speeches about MRSA

Sponsors

— We informed many companies of out project — Some of them decided to help us out. — These are our sponsors

Thank you