Synthetic molecular recognition How we can efficiently screen the - - PowerPoint PPT Presentation

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Mar 10, 2024 269 likes •331 views

Synthetic molecular recognition How we can efficiently screen the proper polymer-SWCNT constructs for specific analyte? Selective evolution of serotonin-specific ssDNA-SWCNT constructs Experimental DNA library = serotonin-binding sequences +

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Synthetic molecular recognition

How we can efficiently screen the proper polymer-SWCNT constructs for specific analyte?

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Selective evolution of serotonin-specific ssDNA-SWCNT constructs

Experimental DNA library = serotonin-binding sequences + non-specific sequences Control DNA library = non-specific sequences

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Fluorescence intensity response of sensor candidates upon serotonin (5-HT) addition

Best nIRHT nanosensor showed ΔF/F = 1.95

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Selectivity of nIRHT nanosensors

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nIRHT nanosensor stably detects serotonin diffusion in acute brain slice nIRHT nanosensor showed reversibility and sub-second response (at least)