Svjetlana Deki Survival of ESKAPE pathogen Acinetobacter baumannii - PowerPoint PPT Presentation

IWA 10 th Eastern European Young Water Professionals Conference – Zagreb 2018 Sponsors: Svjetlana Dekić Survival of ESKAPE pathogen Acinetobacter baumannii in water media of different temperature and pH

Acinetobacter baumannii • Gram negative non-sporogenic coccobacillus • Emerging human opportunistic pathogen • Infections in hospital environment

Acinetobacter baumannii in the environment • Hospital and municipal wastewater • Wastewater treatment plants • Natural waters (Seine, Sava) • Soil contaminated with human solid waste

Aim • Examine the survival of A. baumannii in different temperature and pH conditions in order to predict the behavior of this pathogen inside and outside hospital setting

Material and methods • 4 environmental and 1 clinical isolate selected according to their antibiotic resistance profile during 7 weeks Acquired Antibiotic susceptibility Isolate Clonal origin carbapenemase profile OB4138 IC2 OXA-23 XDR IN39 IC2 OXA-23 MDR EF7 IC2 OXA-23 PDR EF8 IC2 OXA-23 XDR EF11 S unclustered no

Temperature

pH

Results - temperature IN39 Spring water EF7 140 EF8 EF11 120 • 50 ° C survival 2 days in SW, 5 OB 4138 Survival (%) 100 days in NB 80 60 40 20 0 IN39 NB 1:100 -20 4 22 35 44 EF7 Temperature ( ° C) 140 EF8 120 EF11 Survival (%) OB 4138 100 • 63 ° C survival 1 hour in SW, 2 80 hours in NB (pasteurization 60 procedure 63 ° C/30min) 40 20 0 -20 4 22 35 44 Temperature ( ° C)

Results pH Spring water 22 ° C pH 7 9.0 IN39 EF7 EF8 EF11 OB1438 8.0 • pH 5 and 10 no multiplication 7.0 and no drop log CFU/mL 6.0 5.0 4.0 3.0 NB 1:100 22 ° C pH 7 0 5 10 15 20 25 30 35 40 45 9.0 Days 8.0 7.0 log CFU/mL • pH 2 survival up to 3 hours 6.0 IN39 EF7 EF8 EF11 OB1438 • pH 12 up to 5 hours in SW, 24 5.0 hours in NB 4.0 3.0 0 5 10 15 20 25 30 35 40 45 Days

Acinetobacter baumannii • A. baumannii forms smaller translucent colonies together with larger opaque variants

Conclusion • A. baumannii prefers nutrient-rich environment • Optimal conditions for the survival of A. baumannii are room temperature and neutral pH • A. baumannii survives a wide range of temperature and pH values that are unfavorable to most other mesophilic non- sporogenic bacteria • Multi-drug resistant A. baumannii isolates could survive better in harsh environmental conditions

This work was financed by the Croation Science Foundation under the project IP- 2014-09-5656 „Natural habitat of clinically important Acinetobacter baumannii ” . https://www.pmf.unizg.hr/naturaci