Subproject of Diagnosis and control of bacterial pathogen agent of - - PowerPoint PPT Presentation

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Subproject of Diagnosis and control of bacterial pathogen agent of - - PowerPoint PPT Presentation

Feb 25, 2024 459 likes •611 views

Free university of brussels University of Antananarivo LOOKING FOR QUORUM SENSING QUENCHERS : A PROMISING THERAPEUTIC APPROACH AGAINST BACTERIAL INFECTION Subproject of Diagnosis and control of bacterial pathogen agent of

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LOOKING FOR QUORUM SENSING QUENCHERS : A PROMISING THERAPEUTIC APPROACH AGAINST BACTERIAL INFECTION

Subproject of « Diagnosis and control of bacterial pathogen agent of pre- and post-harvest rice and potato in Madagascar »

Free university of brussels University of Antananarivo

Project promotors : Pr Mondher El Jaziri, Pr Pierre Duez Presented by Tsiry RASAMIRAVAKA, MD Financial support: PIC project

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Host lab

  • Laboratoire de biotechnologie vegetale
  • Expertise in molecular biology, plant and microbial biotechnology
  • Lead project to promote technology for sustainable development of

developing country (Burkina faso, Madagascar, …)

  • Global aims and vision:
  • Valorization and preservation of floral biodiversity
  • Consolidation of international collaboration (partnership)
  • Ecomonic development
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The project

  • PIC project : “Diagnosis and control of bacterial

pathogen agent of pre- and post-harvest rice and potato in Madagascar”

  • Subproject : “Looking for Quorum Sensing Quenchers : A

promising therapeutic approach against bacterial infection”

  • Specific aims of this project :
  • Implementation of specialized lab (technology transfer)
  • In phytopathogen diagnosis
  • In the screening of active compound (antibacterial activity and/or anti

quorum sensing)

  • Reserchear formation
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Problems and challenges

  • Why searching for an alterantive struggle against

bacterial infection?

  • Antibacterial molecules are facing limitations and

drawbacks

  • Create a selective pressure (multidrug resistant bacteria)
  • No effect on produced toxins
  • Not appropriate the treatment of infected plant
  • One promising approach is to prevent bacterium

to become pathogenic

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Low cell density High cell density

Harmless bacterium Pathogenic bacteria

Virulence factors ++++ No virulence factors

Ok I see you I’m here guys!!

Cell-to cell communication = Quorum sensing (QS) Interfering with Cell-to cell communication

Nonpathogenic bacteria pathogenic bacteria

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Approach

  • Inhibition of virulence factor expression of bacteria

without killing them

  • Give the necessary time for immune defense to destroy the

pathogenic bacteria

  • Avoid selective pressure
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Approach

  • According to scientific literature, eukaryotes, including

algae and higher plants, produce several compounds that interfere with bacterial quorum sensing (Bjarnsholt et al, 2005; Vandeputte et al., 2010)

  • So we reasonably expect to find active compounds from

Malagasy plants that impaired with bacterial QS

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Results

  • Malagasy endemic plant give promising results
  • Identification of Catechin as One of the Flavonoids from

Combretum albiflorum Bark Extract That Reduces the Production of Quorum-Sensing-Controlled Virulence Factors in Pseudomonas aeruginosa PAO1 (Vandeputte et al., 2010 Applied And Environmental Microbiology)

  • Endemic Malagasy Dalbergia species inhibit quorum sensing in

Pseudomonas aeruginosa PAO1 (Rasamiravaka et al., 2013 Microbiology)

  • Investigation of other Malagasy plants represents a

promising trail in the perspective of finding new anti-QS compounds

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Results

  • Contributions of this partnership for my local lab

and my university ?

  • Lab equipment for advanced research
  • International visibility  opening collaboration with other

laboratory and organization

Before partnership During partnership publications 1 4 published 4 expected

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Results

  • Contributions of this partnership for my local lab

and my university

  • Help establish a screening platform lab of Malagasy

(endemic) plant in order to find new molecules

  • Madagascar present a very huge floral biodiversity (about 80 %

endemic species among 18000 estimated flora)

  • Sustainability of this anti-QS compound screen lab
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Results

  • Contributions of my research for my country!
  • Scientific valorisation of endemic flora
  • New scientific argument for preservation of malagasy

endemic plant (unknown activity of medicinal plant)

  • Scientific expertise to lead research
  • Fondamental scientific data for improvement of struggle

against bacterial infection

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Conclusion

  • Projects
  • Crucial for the development of advanced research in

developing country

  • Advanced scientific data is more useful for north university
  • Help to the establishment of good partnership
  • Improve scientific data exchange
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Project partners

  • ULB/LBV (Belgium partners)
  • Olivier Vandeputte
  • Mondher El jaziri
  • Pierre Duez
  • UA/LBM/IMRA (Malagasy partners)
  • Tsiry Rasamiravaka
  • Christian Rabemanantsoa
  • Abel Andriantsimahavandy
  • Andry Rasamindrakotroka