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Results from the Sorcerer II Global Ocean Sampling Expedition The - - PowerPoint PPT Presentation
Results from the Sorcerer II Global Ocean Sampling Expedition The - - PowerPoint PPT Presentation
Results from the Sorcerer II Global Ocean Sampling Expedition The Sorcerer I I Expedition Global Ocean Sam pling Route www.sorcerer2expedition.org Sam ple Fractionation JTC Sequencer Lab Capacity: 240,000 sequences/day or 80 million
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The Sorcerer I I Expedition
Global Ocean Sam pling Route
www.sorcerer2expedition.org
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Sam ple Fractionation
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JTC Sequencer Lab
Capacity: 240,000 sequences/day or 80 million lanes/year at 24 runs per day
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Prelim inary Analysis: Nova Scotia through Galapagos
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W ater Tem perature: Nova Scotia through Galapagos Islands
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Clustering of GOS sites: Reads incorporated into significant scaffolds
10 9 7 8 3 2 6 4 5
Cold Water Samples Warm Water Samples
0 19 17 1 31 16 15 34 29 27 30 21 22 25 14 23
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The Biodiversity of Each New Region is Different
Flamingo Pond (Galapagos) Flamingo Pond (Galapagos) Eastern U.S. Eastern U.S. Seaboard Seaboard Galapagos Galapagos North Atlantic North Atlantic Sargasso Sea and Sargasso Sea and Yucatan Channel, Mexico Yucatan Channel, Mexico Central America Central America Panama Canal Panama Canal
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Proteorhodopsin Multiple Alignment
97 GI proteins 2350 VI proteins 22 pages total
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Proteorhodopsins Vary by Region
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Sequenced Prochlorococcus genome Highly similar sequence Group of related prochlorococcus strains Highly conserved protein sequences
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GOS protein analysis
1 2 3 4 5 6 GOS Known proteins (NCBI+TGI+ENS)
GOS nearly doubles the number of proteins
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