Research activities at the Research activities at the Department - PowerPoint PPT Presentation

Research activities at the Research activities at the Department supported by Department supported by IPICS/SIDA IPICS/SIDA John WASSWA John WASSWA Department of Chemistry Department of Chemistry Makerere University Makerere University 1

Prologue Prologue  IPICS support since - 1999  IPICS support since - 1999  Human resource capacity  Human resource capacity  Environmental Chemistry ( Pesticide  Environmental Chemistry ( Pesticide Project UG 01) and Coordination Project UG 01) and Coordination Chemistry (Project) Chemistry (Project) 2

PESTICIDE PROJECT PESTICIDE PROJECT - Characterisation Of Pesticide Characterisation Of Pesticide Residues In Biota, Water And Residues In Biota, Water And Sediments In Lake Victoria Sediments In Lake Victoria –IP IP Prof. B. T. Kiremire Prof. B. T. Kiremire  Aim at strengthening capacity for  Aim at strengthening capacity for research in Pesticides at Makerere research in Pesticides at Makerere University through active research University through active research cooperation and collaboration with cooperation and collaboration with institutions within the region and in institutions within the region and in Sweden. Sweden. 3

Infrastructural development Infrastructural development  Prior to 2000, our residue analysis work was done in  Prior to 2000, our residue analysis work was done in the laboratories of the Faculty of Veterinary Medicine the laboratories of the Faculty of Veterinary Medicine and GAL. and GAL.  2001, started to equip a fully-fledged pesticide residue  2001, started to equip a fully-fledged pesticide residue laboratory within the department of chemistry laboratory within the department of chemistry  By the end of the year 2002, our pesticide laboratory  By the end of the year 2002, our pesticide laboratory possessed three functional GC units. possessed three functional GC units. ◦ A UNICAM AT 610, equipped with only the FID and 2 Perkin A UNICAM AT 610, equipped with only the FID and 2 Perkin Elmer units of Model PE 8500 one of which is equipped with Elmer units of Model PE 8500 one of which is equipped with ECD/FID and the other equipped with only the FID- Support ECD/FID and the other equipped with only the FID- Support from GTZ from GTZ 4

 The laboratory also possessed two gas  The laboratory also possessed two gas generators for independent supply of generators for independent supply of Hydrogen and Nitrogen and an HPLC -GTZ Hydrogen and Nitrogen and an HPLC -GTZ  In 2003, through IPICS funds, we secured a  In 2003, through IPICS funds, we secured a new GC unit (VARIAN CP-3800) equipped new GC unit (VARIAN CP-3800) equipped with both an ECD and a NPD. with both an ECD and a NPD. 5

 GPC, Pressure controlled RotarVap, De- ioniser, Ultrasonic bath Relevant Glassware 6

 In 2008 secured an Agilent GC-MS  In 2008 secured an Agilent GC-MS through support from OPCW through support from OPCW  2013 Secured HPLC –Through SIDA  2013 Secured HPLC –Through SIDA 7

Capacity building Capacity building  Trained 10 MSc students and 2 PhDs in  Trained 10 MSc students and 2 PhDs in residue analysis residue analysis  On going 4 PhDs  On going 4 PhDs 8

STUDENT QUALIFICATION CURRENT POSITION John Wasswa MSc, PhD (Mak) Staff Chem Dept (Mak) Gabriel Kasozi MSc (Mak), PhD (UF-USA) Staff Chem Dept (Mak) Sunny Mbabazi MSc (Mak) Principal Analyst (DGAL) Christine Kyarimpa MSc (Mak) Staff Chem Dept (KYU), PhD Student (Mak) Kenneth Arinaitwe MSc (Mak) Staff Chem Dept (Mak), PhD Student (Mak) Patrick Ssebugere MSc (Mak) Staff Chem Dept (Mak), PhD Student (Mak) Democratic Republic of Congo (PhD Democratic Republic of Congo (PhD Muyisa Kavatsurwa Muyisa Kavatsurwa MSC (Mak) MSC (Mak) fellow) fellow) Séverin Edward Mubiru MSc (Mak) Staff Chem Dept (Mak), PhD Student (Mak) Hasifa Nakayima MSc (Mak) QC (Quality Chemicals) Stella Nanyonga MSc (Mak) Staff Chem Dept (KYU), PhD Student Edwige Kampire Kigali Institute of T echnology (PhD MSc (Mak) fellow) Paul Mukasa MSc (Mak) Staff Chem Dept (Bugema) Benon Nturishize MSc (MUST)-Access to On going facilities 9

 Facilities benefited other research groups  Facilities benefited other research groups (natural products and the fish project) (natural products and the fish project)  Hosted a number of colleagues from  Hosted a number of colleagues from within the region (University of Dar es within the region (University of Dar es Salaam, UON-Kenya Salaam, UON-Kenya  Uganda - member ANCAP since its  Uganda - member ANCAP since its inception in ANCAP inception in ANCAP 10

 Hosted 3 summer schools (2002, 2005,  Hosted 3 summer schools (2002, 2005, 2009) 2009)  Regional workshop on DDT(2004)  Regional workshop on DDT(2004)  International conference ( SETAC-AFRICA  International conference ( SETAC-AFRICA 2009 ) 2009 )  14 publications in internationally  14 publications in internationally recognise journals recognise journals  Over 20 conference papers  Over 20 conference papers 11

Collaborators Collaborators  Prof. P  Prof. P . Kizza; University of Florida . Kizza; University of Florida  Prof. Shem Wandiga; UON  Prof. Shem Wandiga; UON  Prof. Negguse Mergesa; University of Addis Ababa  Prof. Negguse Mergesa; University of Addis Ababa  Prof. Kishimba (RIP); University of Dar-es Salaam,  Prof. Kishimba (RIP); University of Dar-es Salaam,  Prof. Mark Zaranyika University of Zimbabwe  Prof. Mark Zaranyika University of Zimbabwe  ANCAP Family  ANCAP Family 12

Current Research Current Research SIDA-Mak SIDA-Mak  Multi-Matrix Contamination Levels, Trends  Multi-Matrix Contamination Levels, Trends and Biomagnifications of Persistent Organic and Biomagnifications of Persistent Organic Pollutants and Pesticides in Fish Food Web of Pollutants and Pesticides in Fish Food Web of Lake Lake Victoria (Uganda) Victoria (Uganda) ◦ Aim: to establish the spatial and temporal trends Aim: to establish the spatial and temporal trends of selected legacy POPs (including emerging of selected legacy POPs (including emerging POPs) in ambient air and precipitation samples POPs) in ambient air and precipitation samples taken from the Ugandan watershed of L. Victoria taken from the Ugandan watershed of L. Victoria 13

Collaborators Collaborators Derek C.G. Muir 1 , Camilla T Derek C.G. Muir 1 , Camilla T eixeira 1 , T eixeira 1 , T om om Harner 2 , Phil Fellin 3 , Robert E. Hecky 4 Harner 2 , Phil Fellin 3 , Robert E. Hecky 4 1 Envi ronment Canada, Burlington, ON 1 Envi ronment Canada, Burlington, ON 2 E n v ironment Canada, Toronto, ON 2 E n v ironment Canada, Toronto, ON 3 A i r ZoneOne Inc., Mississauga, ON 3 A i r ZoneOne Inc., Mississauga, ON 4 B i ology Department, University of 4 B i ology Department, University of Minnesota, Duluth Minnesota, Duluth 14

Equatorial Africa Deposition Network Equatorial Africa Deposition Network  AFull-sized project of the ( GEF ) spanning both  AFull-sized project of the ( GEF ) spanning both the Land Degradation and International Waters the Land Degradation and International Waters focal areas. focal areas. ◦ Implemented by the United Nations Environment Implemented by the United Nations Environment Programme ( UNEP ) Programme ( UNEP )  Aim: to establish a working dialogue between  Aim: to establish a working dialogue between equatorial African Governments that focuses on equatorial African Governments that focuses on the transboundary transport of major the transboundary transport of major macronutrients in order to foster regional macronutrients in order to foster regional cooperation to advocate for changes in national cooperation to advocate for changes in national and regional rural development programs. and regional rural development programs. 15

Future work Future work  Deposition studies on POPs, emerging  Deposition studies on POPs, emerging pollutants and other major pollutants and other major macronutrients in other water bodies of macronutrients in other water bodies of Uganda Uganda 16

COORDINATION CHEMISTRY COORDINATION CHEMISTRY PROJECT PROJECT Currently “the GREEN CHEMISTRY Currently “the GREEN CHEMISTRY PROJECT” supported by SIDA/ PROJECT” supported by SIDA/Mak Mak – IP Prof. H. IP Prof. H. Ssekaalo Ssekaalo  MAJOR ACTIVITIES  MAJOR ACTIVITIES ◦ Development of catalysts for various synthetic Development of catalysts for various synthetic processes processes ◦ Interactions of NPS derivatives with metal Interactions of NPS derivatives with metal complex species and their applications complex species and their applications 17

Major Equipment under Major Equipment under IPICS/SIDA IPICS/SIDA  UV-VIS Spectrophotometer (Procured using IPICS  UV-VIS Spectrophotometer (Procured using IPICS Autumn money) Autumn money)  Magnetic susceptibility balance  Magnetic susceptibility balance  High pressure autoclave reactor  High pressure autoclave reactor  Automatic potentiometeric titrator  Automatic potentiometeric titrator  Rotar evaporator  Rotar evaporator  Fume hood  Fume hood  Polarization microscope  Polarization microscope  Centrifuge  Centrifuge  Analytical balance  Analytical balance  pH/Ion selective meter and electrodes  pH/Ion selective meter and electrodes  Computer and printer  Computer and printer 18