Pr Present enters ers: : Yu Xiaoyu, Zhang Shu, Wang Xuan, Hou - - PowerPoint PPT Presentation
Pr Present enters ers: : Yu Xiaoyu, Zhang Shu, Wang Xuan, Hou Yuelong Background Utilization World Chemical Ratio: Population: Fertilizer: 30- 40% 7 billion $185 billion absorbed Fertilize Directional Directionally Fertilization
Pr Present enters ers: :
Yu Xiaoyu, Zhang Shu, Wang Xuan, Hou Yuelong
Directional Fertilization
World Population: 7 billion Chemical Fertilizer: $185 billion Utilization Ratio: 30- 40% absorbed Fertilize Directionally Peanuts with Molybdenate
Collecting Mo
Delivery
Kill Switch
Simulation
Analysis
Human Practice
Directional Bacterial Fertilizer Simulation
Collecting Mo
Delivery
Kill Switch
Simulation
Analysis
Human Practice
ModA： Highest substrate Kd values Periplasmic
Hollenstein K, Frei D C, Locher K P. Nature, 2007, 446(7132): 213-216.
INP: Surfave display system
Clone, expression and purification of ModA
Left: expression and purification of ModA Right: ModA binding Mo Part: eliminate some sites of endonucleases
INPN > INPNC: the activity of small passenger Future plan: fusion protein
Collecting Mo
Delivery
Kill Switch
Simulation
Analysis
Human Practice
malate & succinate
McfR
Malate succinate
Promoter BBa_J23100 Insulator RBS McfR
Chassis: BL21
Coating contents of capillary tubes
PR5a：promoter&RBS
No chemotaxis
PA2652：2011-Imperial London
More universal function
Chemorecptors: P. putida KT2440 pp4658
Conserved
Attractants：McpS－LBR＋ malate / succinate
Pineda-Molina, Estela, et al. Proceedings of the National Academy of Sciences 109.46 (2012): 18926-18931.
Conserved sequences assemble a super E.coli Prometheus
Collecting Mo
Delivery
Kill Switch
Simulation
Analysis
Human Practice
at a certain time
Stable State IPTG 1 LacI CI 1 MazF
Suicide State IPTG LacI 1 CI MazF 1
Background Inference:
system
Model “Countdown Time”
Promoter 2
Collecting Mo
Delivery
Kill Switch
Simulation
Analysis
Human Practice
chemotaxis in soil
curve
Sauer, D., Kuzyakov, Y. & Stahr, K. J. Plant Nutr. Soil Sci 169, 360-362 (2006).
Parameter Value Origin Velocity 10μm/s Berg, H.C.(ED.).(2004). E.coil in Motion. Springer run 1.4s Brown, D.A.,& Berg, H.C., PANS, 1974, 71(4), 1388-1392 run 3.0s tumble 0.14s tumble 0.19s Exudate distribution formula Shown above Estimation KD 3.5mM Fit from wet experiment Surface concentration(lg)
1 M Set order
Brown, D. A. & Berg, H. C. PNAS, 71, 1388-1392 (1974).
Violet points tend towards root The screenshots of the visualized program
1000 bacteria 1 hour 5*5*5 cm3 root space
1 hour
80% 40%
~2500 sec
Bell-shaped curves
Put the influence of the complex environment into our model Simple, but innovative!
A little step of simulation, a new view of synthetic biology.
Collecting Mo
Delivery
Kill Switch
Simulation
Analysis
Human Practice
Human practice
Change
Policy & Practice
Why BNU? Education Beijing Normal University! Social Edu-justice More than 100 years
Rural Areas in Shandong 2014, August Urban Areas in Beijing 2014, November Lectures: Biology, synthetic biology and iGEM Participate Members: Wang Xuan, Huang Li, Liu Bing, Huang Shengnan, Li Siyao, Ma Di
Answer by 47 senior high students in rural areas and 27 in urban areas 17 % still received no lab courses About 70% do not know any subareas
BNU-China team members with Wang Yue
Review 2.Interviewed Wang Yue 3.Suggestions giving
Wang Yue The director of the journal of Chinese Health Law
Literature Review
Environment
Module
Efficiency
Interview
Economy
Shandong Academy
Science
Collaboration
China iGEM Meet up in Wuhan
Conference
Kyoto, Peking, OUC team
Communication
Oct Shidaerfu
Future iGEMers
August Weifang
Rural Project
Oct BNU
Open Day of BNU-China
benqiong Support us to our iGEM journey. Huo liang, Ren qidong & Zhang yahui Gave us advises doing experiment. Dr.Du Guoguang and an Zhao Shifeng, Helped us doing laser scanning of peanuts’ roots. Dai Cong and his family, Helped us get peanuts samples.
Gave us advise about our bright future.