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Sep 04, 2022 113 likes •333 views

Genuine Manuka Honey!? ? Nicole Beitlich Prof. Dr. K. Speer Food Chemistry Technische Universitt Dresden, Germany 1 Professorship of Special Food Chemistry / Food Production Manuka honey (Leptospermum scoparium) Healing honey

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Genuine Manuka Honey!?

Nicole Beitlich

  • Prof. Dr. K. Speer

Food Chemistry Technische Universität Dresden, Germany

?

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Manuka honey (Leptospermum scoparium)

 Antioxidant (Inoue et al. 2005)  Antiinflammatory (Leong et al. 2011)  Immunostimulatory (Tonks et al. 2003)  Antitumor potential (Fernandez-Cabezudo et al. 2013)  Antiulcer and wound healing (Doerler et al. 2012)  Antibiotic, antiviral & antibacterial (Adams et al. 2008, Mavric et al. 2008)

Professorship of Special Food Chemistry / Food Production 22-Nov-2016

  • N. Beitlich – Genuine Manuka Honey!?

Adams et al. (2008) Carbohyd. Res. 343, 651659; Doerler et al. (2012) J. Dtsch. Dermatol. Ges. 10, 624-632; Fernandez-Cabezudo (2013) Plos One. 8, e55993; Inoue et al. (2005) J. Sci. Food Agric. 85, 872-878; Leong et al. (2011) J. L. Innate Immun. 18, 459-466; Mavric et al. (2008) Mol. Nutr. Food Res. 52, 483-489; Tonks et al. (2003) Cytokine. 21, 242-247

‘Healing honey’ dihydroxyacetone methylglyoxal (MGO) Non-peroxide antibacterial activity (= NPA)

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Manuka honey (Leptospermum scoparium)

Professorship of Special Food Chemistry / Food Production 22-Nov-2016

  • N. Beitlich – Genuine Manuka Honey!?

Adams et al. (2008) Carbohyd. Res. 343, 651659; Doerler et al. (2012) J. Dtsch. Dermatol. Ges. 10, 624-632; Fernandez-Cabezudo (2013) Plos One. 8, e55993; Inoue et al. (2005) J. Sci. Food Agric. 85, 872-878; Leong et al. (2011) J. L. Innate Immun. 18, 459-466; Mavric et al. (2008) Mol. Nutr. Food Res. 52, 483-489; Tonks et al. (2003) Cytokine. 21, 242-247

 Very expensive honey  Production: 11.700 t  Sale: 10.000 t

 Fraud ?!

‘Healing honey’

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4 Professorship of Special Food Chemistry / Food Production 22-Nov-2016

  • N. Beitlich – Genuine Manuka Honey!?

Authentication

Manuka Kanuka Manuka

Blossom  Identical pollen  No differentiation possible  Natural mixing

MGO

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5 Professorship of Special Food Chemistry / Food Production 22-Nov-2016

  • N. Beitlich – Genuine Manuka Honey!?

Authentication

 Conversion MGO ⟺ DHA  Adding MGO and/or DHA

DHA MGO

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6 Professorship of Special Food Chemistry / Food Production 22-Nov-2016

  • N. Beitlich – Genuine Manuka Honey!?

Authentication  Alternative methods are required  Secondary plant metabolites are promising

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 Authentication of 24 European monofloral honeys (sunflower, chestnut, cornflower, …) using secondary plant metabolites

Our working group

Professorship of Special Food Chemistry / Food Production 22-Nov-2016

  • N. Beitlich – Genuine Manuka Honey!?

Analysis of monofloral European honeys 1 h / sample Volatiles HS-SPME-GC/MS Nonvolatiles UHPLC-PDA-MS/MS

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MPI Manuka honey science programme

Professorship of Special Food Chemistry / Food Production 22-Nov-2016

  • N. Beitlich – Genuine Manuka Honey!?

“The Ministry for Primary Industries (MPI) is leading and managing a science programme to support the development of a robust science-based definition for monofloral manuka honey.” “… attributes (chemical and/or DNA) …”

MPI (2016). Manuka honey science programme.

 Started in 2014  Will be completed by the end of 2016  Defined monofloral honeys of varying floral types

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9 Professorship of Special Food Chemistry / Food Production

HAHSUS method

22-Nov-2016

  • N. Beitlich – Genuine Manuka Honey!?

Honey Authentication by HS-SPME-GC/MS and SPE-UHPLC-MS/MS

combined with Statistics

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10 Professorship of Special Food Chemistry / Food Production 22-Nov-2016

  • N. Beitlich – Genuine Manuka Honey!?

HAHSUS methods

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11 Professorship of Special Food Chemistry / Food Production 22-Nov-2016

  • N. Beitlich – Genuine Manuka Honey!?

Beitlich et al. (2016) Fluorescent Pteridine Derivatives as New Markers for the Characterization of Monofloral Genuine New Zealand Manuka (Leptospermum scoparium) Honey. Journal of Agricultural and Food Chemistry. DOI 0.1021/acs.jafc.6b03984

TLC Screening

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12 Professorship of Special Food Chemistry / Food Production 22-Nov-2016

  • N. Beitlich – Genuine Manuka Honey!?

Beitlich et al. (2016) Fluorescent Pteridine Derivatives as New Markers for the Characterization of Monofloral Genuine New Zealand Manuka (Leptospermum scoparium) Honey. Journal of Agricultural and Food Chemistry. DOI 0.1021/acs.jafc.6b03984

TLC Screening

3,6,7-trimethyl-2,4(1H,3H)- pteridinedione 6,7-dimethyl-2,4(1H,3H)- pteridinedione

1 2

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13 Professorship of Special Food Chemistry / Food Production 22-Nov-2016

  • N. Beitlich – Genuine Manuka Honey!?

HS-SPME-GC/MS

TIC profiles

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14 Professorship of Special Food Chemistry / Food Production 22-Nov-2016

  • N. Beitlich – Genuine Manuka Honey!?

HS-SPME-GC/MS

TIC profiles

 Volatile marker compounds

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15 Professorship of Special Food Chemistry / Food Production 22-Nov-2016

  • N. Beitlich – Genuine Manuka Honey!?

HS-SPME-GC/MS & UHPLC-MS/MS

PDA profiles, λ 254 nm

 Nonvolatile marker compounds

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16 Professorship of Special Food Chemistry / Food Production 22-Nov-2016

  • N. Beitlich – Genuine Manuka Honey!?

Nonvolatile markers Volatile markers  Proved by significance test, boxplots, and PCA

Beitlich et al. (2014) Differentiation of manuka honey and kanuka honey from jelly bush honey using HS-SPME- GC/MS and UHPLC-PDA-MS/MS. Journal of Agricultural and Food Chemistry. 62, 6435-6444.

 Clear differentiation between manuka honey and kanuka honey

HS-SPME-GC/MS & UHPLC-MS/MS

Score plot

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17 Professorship of Special Food Chemistry / Food Production 22-Nov-2016

  • N. Beitlich – Genuine Manuka Honey!?

Manuka-Kanuka-Mixtures

 Definition of monofloral honey: “…if it comes wholly or mainly from that particular source…” (Codex Stan 12-1981)  Our definition: monofloral manuka honey >70%  Relative chart on the basis of special markers were developed

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18 Professorship of Special Food Chemistry / Food Production 22-Nov-2016

  • N. Beitlich – Genuine Manuka Honey!?

Classification of commercial manuka honeys

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 Summary

 Clear differentiation of manuka honey from kanuka honey and other NZ honeys  Validated method for manuka honey authentication  HAHSUS – Honey Authentication by

HS-SPME-GC/MS and UHPLC-PDA-MS/MS

combined with Statistics

Professorship of Special Food Chemistry / Food Production 22-Nov-2016

  • N. Beitlich – Genuine Manuka Honey!?
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20 Professorship of Special Food Chemistry / Food Production 22-Nov-2016

  • N. Beitlich – Genuine Manuka Honey!?

Nicole Beitlich phone: + 49 351 463 37868 e-mail: nicole.beitlich@chemie.tu-dresden.de

  • Prof. Dr. Karl Speer

Phone: + 49 351-463 33132 e-mail: karl.speer@chemie.tu-dresden.de

Thank you for your attention!