SLIDE 1
Jan Felix Drexler Institute of Virology, University of Bonn
How old are hepadnaviruses – endogenous bird & reptile variants well >60 million years
Suh, PLoS Gen, 2015
New insight into HBV evolution Jan Felix Drexler Institute of - - PowerPoint PPT Presentation
New insight into HBV evolution Jan Felix Drexler Institute of - - PowerPoint PPT Presentation
New insight into HBV evolution Jan Felix Drexler Institute of Virology, University of Bonn How old are hepadnaviruses endogenous bird & reptile variants well >60 million years Suh, PLoS Gen, 2015 Consistent with diverse
SLIDE 2
SLIDE 3
Consistent with diverse hepadnaviruses in lower vertebrates
Dill, J Virol, 2016
Mammal-like?
SLIDE 4
Hepatology, 2012
Slow long-term evolution: Mummy HBV near-identical to contemporary strains
SLIDE 5
Extant bats are old mammalian lineages – introduction of ancestral HBV into mammals?
Simmons, Science 2005
SLIDE 6
Screening of 3,000 bats for HBV
Drexler/Geipel et al., 2013, PNAS
SLIDE 7
Roundleaf bat
- H. Ruber
RBHBV
Tent-making bat
- U. Bilobatum
TBHBV
Horseshoe bat
- R. Alcyone
HBHBV
natalie's Life List, inaturalist.org
3 viruses
1 2 3
SLIDE 8
Relevance of bats - Chiropteran hosts harbor largest hepadnavirus diversity within mammals
Rasche et al., Curr Opinion Virol, 2016
Two HBV species in primates: HBV WMHBV
SLIDE 9
Primate HBV ancestors: Acquisition of bat viruses by monkeys?
Rasche et al., Curr Opinion Virol, 2016
SLIDE 10
Lack of studies into New World non-human primates
123 animals 9 species 4 studies in 45 years
SLIDE 11
Brazil: 5th largest country Highest primate diversity worldwide
Brazil 118 species
SLIDE 12
Screening of ca. 100 NHP A new primate HBV species: CMHBV
Dominguez/König/Rasche et al., unpublished
SLIDE 13
Potentially chronic infection: core deletions and no signs
- f inflammation
Dominguez/König/Rasche et al., unpublished
SLIDE 14
Deep HBV sister lineages in new world monkeys
Dominguez/König/Rasche et al., unpublished
SLIDE 15
Evidence for ancient co- speciation of primate hepadnaviruses and their hosts – but not for HBV
Dominguez/König/Rasche et al., unpublished
SLIDE 16
Ancestral state reconstructions
Primate hepadnavirus
- rigins in
NHP HBV in humans?
Dominguez/König/Rasche et al., unpublished
SLIDE 17
Hypothesis testing
HBV may have evolved in hominoid ancestors 17 million years ago
Dominguez/König/Rasche et al., unpublished
You are here!
SLIDE 18
No evidence for ancestry of F/H in American monkeys Introduction along human migration?
Dominguez/König/Rasche et al., unpublished
SLIDE 19
In sum
- Hepadnaviruses are an extremely old
virus family
- Primate hepadnaviruses may be the
result of a host switch millions of years ago
- HBV likely evolved in hominoid
ancestors, preceding the human stem lineage
- Divergent New World genotypes F/H
may originate from pre-historic human migration
SLIDE 20
Virology, Bonn Christian Drosten Victor Max Corman Monika Eschbach-Bludau Sebastian Brünink Tobias Bleicker Andrea Rasche Marcel Müller FLI, Riems Rainer Ulrich Martin Beer Ulm Elisabeth Kalko Marco Tschapka Stellenbosch Wolfgang Preiser Sonja Matthee Kruger NP Danny Govender EMC, Rotterdam Ab Osterhaus Thijs Kuiken Debby van Riel JMA van den Brand Virology, Berlin Detlef Krüger Virology, Giessen Dieter Glebe Virology, Heidelberg, Ralf Bartenschlager Virology, Hannover Georg Herrler Bernhard Nocht Institut, HH Thomas Kruppa Jonas Schmidt-Chanasit RIVM, Netherlands Chantal Reusken ILRI, Kenya Jörg Jores
Funded by
KCCR, Kumasi Augustina Annan Samuel Oppong Mexico Álvaro Aguilar Setién Veterinary Medicine, Salvador Roberto Franke Eduardo Netto Chumakov Institute, Moscow Alexander Lukashev CIRMF, Franceville Eric Leroy AFMB, Marseille Bruno Coutard