, - - PowerPoint PPT Presentation

Έρευνα και ανάπτυξη προϊόντων

Κωνσταντίνος Γαρδίκης, MSc, PhD, Scientific Affairs Director

APIVITA BRAND

APIVITA is a highly sophisticated natural cosmetics brand, developing and producing natural, effective and holistic cosmetics and well being products and services

INSPIRATIONS

BEE SOCIETY GREEK FLORA HIPPOCRATES

.

OUR DRIVING AXIS

• 1. Our Brand Identity
• 2. APIVITA strategic goals on

Raw Materials Development

• 3. Market research defining

Cosmetics and Health market status

• 4. Scientific Collaborations &

Research Programs

• 5. Cosmetics Legislation

Evolution

• 6. Innovation

Product Development Platform … driven by Value Innovation

WHAT MAKES OUR PRODUCTS UNIQUE

36 YEAR RESEARCH & DEVELOPMENT EVOLUTION

Certifications

Scientific collaboration with universities and research institutes for extensive research on Greek plant actives and applications 7 research programs in collaboration with the University of Athens accomplished and 10 in progress In house plant extracts and oils development and production 60 plants studied / 200 extracts developed since 1979 37 years proven experience in natural products development Experience and flexibility leads to more than 100 new product developments every year Extensive knowledge of natural and organic cosmetics (the first in the Greek market to produce natural cosmetics, the first in the Greek pharmacies to produce organic sunscreens). Extensive ingredients testing and deep under- standing 5000 ingredients tested / 1500 ingredients used. New developments in increasing products natural ingredients level such as innovative preservation systems, emulsifying systems, active ingredients.

The department consists of 14 scientists, chemists, pharmacists, chemical engineers and cosmetologists who are in charge of the development of natural raw materials, cosmetic products’ formula and notification & legalization of the products to the countries of company’s operation

A success story of innovation

PRODUCT

NATURAL – EFFECTIVE - HOLISTIC

NATURAL

APIVITA products have formulas with high percentage of natural ingredients 85%-100% , environmentally friendly packaging and they are produced with respect to nature.

WITH WITHOUT

NATURAL

Plant extracts Plant oils Plant Infusions Bee Products Essential oils

*Greek plant, organically cultivated, Hippocrates plants

Parabens Silicones Propylene Glycol Mineral Oil Ethanolamines Phthalates, Nitromusks, Polycyclic Musks

PLANT EXTRACTS

Organic Plants: 99% of the plants we use for the in house production of extracts are from organically certified cultivations Greek Plants: Combining the knowledge of the traditional use and modern scientific research on the Greek biodiversity Hippocrates Plants: Thorough study of his literature brought to light the exact plant species which he used for his therapeutic methods.

Hippocrates Botanical Garden on Kos island

EFFECTIVE

APIVITA’s R&D department has a proven track record of experience in developing effective cosmetics. The ingredients used are on the cutting edge of green cosmetology and fulfills efficacy substantiation requirements Ingredients' activity and effectiveness is measured in house and in collaborations In vivo clinical studies are conducted on the final products in close partnership with research institutes and universities.

Analytical tests Physicochemical tests (pH, Specific gravity etc) UV-Vis spectrophotometry Microbiological tests Chromatographic analysis (HPTLC, HPLC) Qualitative analysis Quantitative analysis Total phenolics (Folin- Ciocalteau method) Total Flavonoids Radical Scavenging (DPPH) Total Phenolics

PROOF OF EFFICACY

Phytochemistry Lab

Biochemistry assays Gel Electrophoresis DNA barcoding Gene expression Allergen identification Genetic fingerprinting Microbiological testing Real Time Polymerase Chain Reaction (qPCR) Elisa Immunosorbent Assay Protein detection

PROOF OF EFFICACY

Biochemistry Lab

Extracellular Molecules Transcription Regulators Cell growth and Proliferation Apoptosis Response to Stress

Ageing Oxidative Stress Hydration Inflammation

200 API - Genes PROOF OF EFFICACY

Gene Database

Results

• Skin moisturization is improved by 18% after the

first application and by 21%, after 28 days

• 24 h , Skin moisturization
• TEWL is decreased by 18% after 28 days
• Skin elasticity is improved by 16% after 28 days

Results

• Skin’s density was improved at 87% of the

volunteers after 46 days

• Skin’s tone was improved at 97% of the volunteers

after 46 days

• Skin’s texture was improved at 90% of the

volunteers after 46 days

PROOF OF EFFICACY

In-vivo tests

HOLISTIC

HOLISTIC INSPIRED BY HIPPOCRATES

Multifunctional products which offer more than one action & cover all skin's needs. Maintain skin’s health and beauty. Formulas enriched with 100% pure organic essential

• ils in order to enhance a feeling of well being

Holistic approach on health and beauty based on Hippocrates Philosophy The teaching of Hippocrates on Experience, Methodology,Discipline and Evidence is a key inspiration on new product development

INGREDIENTS

NATURAL – EFFECTIVE - HOLISTIC

PLANT INFUSIONS

Replacing water with herbs infusions to enhance effectiveness

Greek Wild rose Infusion Antioxidant anti-aging action Rosemary water Toning action Calendula (marigold) water Calming and soothing action action Chamomile water Anti-irritant and soothing action

Mountain Tea infusion

INNOVATION

• Total phenolic content is assessed by Folin-Ciocalteau assay
• The ability of the infusion to scavenge free radicals is measured by DPPH method

EFFECTIVE PLANT INFUSIONS

Effectiveness of herb infusions is measured by scientific methods

0,05 0,1 0,15 0,2 Sideritis scardica Sideritis raeseri Sideritis perfoliata Sideritis blend Green tea

Trolox equivalent

Antioxidant activity

• Sideritis scardica/raeseri/perfoliata
• (PATENT USE)

SIDERITIS INFUSION PATENT

Propolis (PATENT METHOD) SOPHISTICATED SKIN DELIVERY SYSTEMS : Propolis Encapsulation In Cyclodextrins

EFFECTIVE BEE PRODUCTS

A patent of innovative extraction method and simultaneous encapsulation in cyclodextrins 8 In Vitro Studies 1. Cell Viability Protection- UV treatment 2. Cell Viability Protection – Heavy metal treatment 3. Cell Metabolism Protection – UV Treatment 4. Cell Metabolism Protection – Heavy metal treatment 5. Cell antioxidant capability- UV treatment 6. Cell antioxidant capability – Heavy metal treatment 7. Collagen synthesis 8. Elastin Synthesis SOPHISTICATED SKIN DELIVERY SYSTEMS : Propolis Encapsulation In Cyclodextrins

EFFECTIVE BEE PRODUCTS

Benefits of a liposomal formulation Royal Jelly forms in presence of soy lecithin (phospholipids) vesicles, the liposomes The active ingredients

• f Royal Jelly are incorporated

either in the core of the vesicle or integrated in the phospholipid bilayer

SOPHISTICATED ‘ SKIN DELIVERY SYSTEMS : Fresh Greek Royal Jelly in Liposomes

EFFECTIVE BEE PRODUCTS

in-vivo Clinical studies  Increase of skin firmness skin in 90% of volunteers  Increase of skin elasticity in 90% of volunteers  Reducing the depth of wrinkles in 85% of volunteers

Growth stimulation of fibroblasts

SOPHISTICATED SKIN DELIVERY SYSTEMS : Fresh Greek Royal Jelly in Liposomes

EFFECTIVE BEE PRODUCTS

RESEARCH PROGRAMS

NATURAL – EFFECTIVE - HOLISTIC

HORIZON 2020 PROGRAMS

28

1st round: 16.000 proposals 1st stage success: 11% 2nd stage success: 16%

HORIZON 2020: MARISURF

Title: Novel, sustainable marine bio-surfactant / bio-emulsifiers for commercial exploitation

• Call: H2020- Blue Growth--‐3--‐2014
• Topic: : Novel marine derived biomolecules and industrial biomaterials
• Type of action: Research and Innovation actions
• Target: Isolation and production of new bio-surfactants and bio-

emulsifiers by environmental pollution degrading bacteria (green biotechnology and sustainability)

• APIVITA interest: Cosmeceutical formulation / green marketing
• Total Budget: 4750000 euro
• APIVITA budget: 198000 euro
• Reimbursement rate: 100%
• Duration: 60 months
• Start date: 9/2015

29

PARTICIPANTS

30

PARTICIPANT NO PARTICIPANT ORGANIZATION NAME COUNTRY 1 (Coordinator) HERIOT WATT UNIVERSITY UK 2 ULSTER UNIVERSITY UK 3 UNIVERSITY OF PATRAS GREECE 4 DEMOCRITUS UNIVERSITY OF THRACE GREECE 5 APIVITA, S.A. GREECE 6 BIO BASE EUROPE PILOT PLANT BELGIUM 7 ECOVER BELGIUM 8 ACONDICIONAMIENTO TARRASSENSE SPAIN 9 NOVA INSTITU Gmbh GERMANY 10 NANOIMMUNOTECH, S.L. SPAIN 11 ETS ITALY 12 MARLOW FOODS UK 13 MACHPIE UK

HORIZON 2020: TASCMAR

Title: Tools And Strategies to access to original bioactive compounds from Cultivation of MARine invertebrates and associated symbionts

• Call: H2020- Blue Growth--‐3--‐2014
• Topic: Novel marine derived biomolecules and industrial biomaterials
• Type of action: Research and Innovation actions
• Target: Isolation and production of new antioxidant molecules or

enhanced extracts from invertebrates

• APIVITA interest: New sustainable and effective raw materials

derived with innovative green methods of extraction

• Total Budget: 6760000 euro
• APIVITA budget: 400000 euro
• Reimbursement rate: 100%
• Duration: 48 months
• Start date: 4/2015

31

PARTICIPANTS

32

HORIZON 2020: ALGAE4A-B

Title: Development of Microalgae-based novel high added-value

products for the Cosmetic and Aquaculture industry

• Call: H2020-MSCA-RISE-2015
• Topic: MSCA-RISE
• Type of action: Marie-Curie actions
• Target: New -omic technologies and new strains for the production
• f high added value cosmeceuticals
• APIVITA interest: New multifunctional enhanced extracts and

isolated enzymes

• Total Budget: 972000 euro
• APIVITA budget: 216000 euro
• Reimbursement rate: 100%
• Duration: 48 months
• Start date: 11/2015

33

PARTICIPANTS

34

PARTICIPANT NO PARTICIPANT ORGANIZATION NAME COUNTRY 1 (Coordinator) FITOPLANCTON MARINO SPAIN 2 APIVITA GREECE 3 CNRS FRANCE 4 AGRICULTURAL UNIVERSITY OF ATHENS GREECE 5 IFAPA SPAIN 6 CCMAR PORTUGAL 7 LIFESEQUENCING SPAIN

FRAMEWORK 7/GREEK FRAMEWORK PROGRAMS

Exploitation of microalgae diversity for the development of novel high added-value cosmeceuticals

AlgaeCom

Agricultural University of Athens (AUA) Participant

Participants

Centre National de Recherches Scientifique (CNRS) APIVITA S.A. FITOPLANCTON MARINO Greece Country France Greece Spain Dept.

• Agric. Biotechn.

Lab.

• Molec. Biology

Dept. Marine plant and biomolecules Scientific Affairs-R&D Dr. Emmanouil Flemetakis Scientist in charge Dr. William Helbert Dr. Konstantinos Gardikis Dr. Carlos Unamunzaga 3 4 2 1 Molecular Biology & Manufacturing

ALGAECOM-TARGET

Extract(s) with important firming/antiageing properties

1

• Setup of biochemistry lab
• APIGENES library development
• Control/proof of effectiveness
• DNA barcoding

2

Contact with a new world of ingredients

3 Incorporation in products 2017

Βudget: 1,570,928.00 euro

• Nannochloropsis gaditana extract (1st time in cosmetics) +
• Propolis patent

PRO-ALGAE PATENT

Bee-based natural products as potential cosmeceutical agents with health promoting properties against UV-induced skin photo-ageing

BeeCosmAge

APIVITA S.A

Participants

Participants

University of Ioannina (UoI Democritus University of Thrace (DuTh) Greece

Country

Greece Greece Depart. Research & Development

Dept.

Pathologic Anatomy Molecular Pharmacology Dr. Konstantinos Gardikis

Scientist in charge

• Dr. Anna Gousia

Dr. Aglaia Pappa 2 1 University of Athens (UoA) Greece Pharmacognosy Dr. Nektarios Aligiannis Nanchang University (NCU) China Food Science & Technology Dr. Liping Luo 3 4 5 Pharmacognosy

• Proof of the uniqueness of Greek bee products

BeeCosmAge-Target

• New series of innovative extracts
• Contact with Chinese academia & market

Βudget: 500,000.00 euro (Greek side)

BeeCosmAge-Dissemination

• European Biotechnology Congress (May 2015, Bucharest)

BeeCosmAge-Dissemination

TOXICITY PROFILING OF PROPOLIS EXTRACTS FROM DIFFERENT GEOGRAPHIC REGIONS OF GREECE AND EVALUATION OF THEIR ANTI-OXIDANT AND ANTI-MUTAGENIC AND ANTI-AGEING PROPERTIES ON HUMAN KERATINOCYTES UNDER UVR EXPOSURE CONDITIONS Voulgaridou Georgia-Persephoni1,2, Alexis Galanis1, Sofia Eftaxia2, Anna Gousia2, Nektarios Aligiannis3, Kostantinos Gardikis4, Panayiotidis Michalis4 and Pappa Aglaia 1*

1Department of Molecular Biology & Genetics, Democritus University of Thrace, Alexandroupolis,Greece 2Department of Pathology Medical School, University of Ioannina,

45110 Ioannina

3Department of Pharmacy, Division of Pharmacognosy & Natural Products Chemistry, University of Athens Athens, 15771 4APIVITA SA, Industrial Park, Markopoulo Mesogaias 19003, Markopoulo Attiki, Athens 5School of Life Sciences, Heriot Watt University, Edinburgh, Scotland

*Correspondance: apappa@mbg.duth.gr

• Polyphenols (June 2015, Malta)

BeeCosmAge-Dissemination

APPLICATION OF GREEN EXTRACTION METHODOLOGIES TO DEVELOP PROPOLIS-BASED NATURAL EXTRACTS AS POTENTIAL COSMECEUTCAL AGENTS STAVROPOULOU M.-I. (1), STATHOPOULOU K. (1), KANAKIS H. (1), GARDIKIS K. (2), ALIGIANNIS N. (1) (1) Department of Parmacognosy and Natural Products Chemistry, Faculty of Pharmacy, University of Athens, Athens 15771,Greece (2) Apivita SA Industrial Park of Markopoulo Mesogaias,19003 Markopoulo Attiki ,Athens ,Greece

Propolis is a resinous material collected by honeybees and has various biological activities (antibacterial, antiviral etc.)[1]. It contains phenolics [2], widely recognized as strong antioxidants and suggest that propolis could be applied topically to prevent and treat skin damages [3]. Moreover, safety hazards, high cost of organic solvents and the increased concern for environmental pollution, led to green technologies and innovative extraction procedures [4-6]. This project aims to develop high-quality cosmeceutical agents, based on Greek propolis involving environmentally friendly techniques.

• Controlled Release Propolis
• (PATENT METHOD)

PROPOLIS PATENT CONTROLLED RELEASE

Cyclodextrin propolis Aqueous core Propolis Polar head

Physicochemical and biological characterization and improvement of natural muds to produce high added-value products

PharmaMuds

APIVITA S.A

Participants

Participants

University of Patras (UPatras) University of Athens (UoA) Greece

Country

Greece Greece Scientific Affairs-R&D

Dept.

Chemical Engineering Medical School/Biological Chemistry Dr. Konstantinos Gardikis

Scientist in charge

• Dr. Ioannis

Tsamopoulos Dr. Paraskevi Moutsatsou 2 1 Technion University (Tech) Israel Chemical Engineering Dr. Avi Nir Anna Lotan (AL) Israel R&D Dr. Liping Luo 3 4 5

• Stable raw materials

PhatmaMuds-Target

• New face masks
• Proof of the efficacy of traditionally used materials

Incorporation in products 2018

Βudget: 300,000.00 euro (Greek side)

• Stabilized Messologi Mud
• (PATENT FORMULATION/USE)

STABILIZED MUD PATENT

APIVITA SCIENTIFIC SYNERGIES ECOSYSTEM

74 Universities and Research Institutes  23 countries 16 million euro total budget

Our global presence

Since 1979

Our global presence

USA Cyprus Japan Spain Greece Romania Benelux UK Croatia Ukraine Bulgaria

14 COUNTRIES

Hong Kong

APIVITA Experience Store

APIVITA Experience Store

Athens International Airport Store

Athens Shop in Shop

Athens Pharmacy corner

INNOVATION

extracton of Greek Propolis for the producton

• f high valued raw material with ant-oxidant and

photo-protectve and ant-aging actvityactvity. a method with minimum energy consumpton and zero waste in order to increase stability, permeability and bio- availability of actve components Greek Royal Jelly encapsulaton in liposomes offers new and enhanced propertes for an-tageing

SPAIN: APIVITA SPAIN S.A.

 APIVITA entered the Spanish market in 2003, initially operating through a distribution agreement  In November 2009, APIVITA established a wholly owned subsidiary  Products are currently placed in:

• 1 flagship store in Madrid open since March 2010
• 87 EI Corte Ingles department stores
• 870 pharmacies
• 5 Hotel & SPA

Key highlights

SPAIN 1 Store 87 El Corte Ingles 870 Pharmacies

Madrid

Flagship Store

El Corte Ingles, Barcelona

INNOVATION

extracton of Greek Propolis for the producton

• f high valued raw material with ant-oxidant and

photo-protectve and ant-aging actvityactvity. a method with minimum energy consumpton and zero waste in order to increase stability, permeability and bio- availability of actve components Greek Royal Jelly encapsulaton in liposomes offers new and enhanced propertes for an-tageing

JAPAN: APIVITA JAPAN

 APIVITA entered the market in 2010, initially operated through a distribution agreement  In November 2013, APIVITA established a wholly owned subsidiary. Currently, since 2015, in joint venture Products are currently placed in:  10 stores in key departments stores Key highlights

Tokyo Flagship Store Jiyugaoka

INNOVATION

extracton of Greek Propolis for the producton

• f high valued raw material with ant-oxidant and

photo-protectve and ant-aging actvityactvity. a method with minimum energy consumpton and zero waste in order to increase stability, permeability and bio- availability of actve components Greek Royal Jelly encapsulaton in liposomes offers new and enhanced propertes for an-tageing

HONG KONG

 Apivita is the market for 10 years  Partnership with IMAGINEX, a leading brand management and distribution company on the luxury market segment of the Great China region, based in Hong Kong  11 shop in shop in key departments stores  Raised market awareness through key advertising campaigns Key highlights

NEW TOWN PLAZA Shatin

INNOVATION

extracton of Greek Propolis for the producton

• f high valued raw material with ant-oxidant and

photo-protectve and ant-aging actvityactvity. a method with minimum energy consumpton and zero waste in order to increase stability, permeability and bio- availability of actve components Greek Royal Jelly encapsulaton in liposomes offers new and enhanced propertes for an-tageing

Romania

 APIVITA is in the market for 6 years  Partnership with SensiBlu SensiBlu owns 80% of the pharmacy channel in Romania  Products are currently placed in:  405 pharmacies - 320 full brand  1 flagship store  2 shop in shop Key highlights

ROMANIA 405 Pharmacies (53 corners)

Romania Pharmacy Corner

ROMANIA New Store Bucharest

INNOVATION

extracton of Greek Propolis for the producton

• f high valued raw material with ant-oxidant and

photo-protectve and ant-aging actvityactvity. a method with minimum energy consumpton and zero waste in order to increase stability, permeability and bio- availability of actve components Greek Royal Jelly encapsulaton in liposomes offers new and enhanced propertes for an-tageing

Cyprus

 APIVITA is in the market for 20 years  Partnership with Drogopharma  Products are currently placed in:

• 250 pharmacies
• 1 experience store open since October 2015

Key highlights

CYPRUS New Experience Store

CYPRUS International Airport

CYPRUS Pharmacy Corner

INNOVATION

extracton of Greek Propolis for the producton

• f high valued raw material with ant-oxidant and

photo-protectve and ant-aging actvityactvity. a method with minimum energy consumpton and zero waste in order to increase stability, permeability and bio- availability of actve components Greek Royal Jelly encapsulaton in liposomes offers new and enhanced propertes for an-tageing

Benelux

 Present since July 2010  Products are currently placed in:

• Belgium (100+ pharmacies)
• Luxembourg
• The Netherlands ( 1 Apivita store open since November 2015)

Key highlights

The NETHERLANDS New Amsterdam Store

BELGIUM 100 Pharmacies

INNOVATION

extracton of Greek Propolis for the producton

• f high valued raw material with ant-oxidant and

photo-protectve and ant-aging actvityactvity. a method with minimum energy consumpton and zero waste in order to increase stability, permeability and bio- availability of actve components Greek Royal Jelly encapsulaton in liposomes offers new and enhanced propertes for an-tageing

Ukraine

 APIVITA is in the market for 4 years  Partnership with Olfa  Products are currently placed in:

• 250 pharmacies
• 2 Apivita stores

Key highlights

UKRAINE 250 Pharmacies

INNOVATION

extracton of Greek Propolis for the producton

• f high valued raw material with ant-oxidant and

photo-protectve and ant-aging actvityactvity. a method with minimum energy consumpton and zero waste in order to increase stability, permeability and bio- availability of actve components Greek Royal Jelly encapsulaton in liposomes offers new and enhanced propertes for an-tageing

Bulgaria

 APIVITA is in the market for 2 years  Partnership with A&D Pharma  Products are currently placed in:

• 60 pharmacies
• Notos Department Stores

Key highlights

Six Senses Spa - Elounda, Greece

HOTEL & SPA

Six Senses Spa - Turkey Me by MELIÁ, Madrid

Thank you