Micropropagation Technology Park Sanjay Saxena Micropropagation - - PowerPoint PPT Presentation

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Micropropagation Technology Park Sanjay Saxena Micropropagation - - PowerPoint PPT Presentation

Micropropagation Technology Park Sanjay Saxena Micropropagation Technology Park at Gurgaon, Haryana MTP MTP is one of the largest tissue culture production units in north India with an annual production capacity of 1.5 million plants


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Micropropagation Technology Park

Sanjay Saxena

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Micropropagation Technology Park at Gurgaon, Haryana

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MTP

  • MTP is one of the largest tissue culture production

units in north India with an annual production capacity of 1.5 million plants

  • The facility is ISO 9001 certified and DBT, Govt. of

India accredited reflecting the quality control and assurance of the lab

  • Micropropagation protocols have been developed

for over 90 species including, forestry species, fruit crops, cash crops, medicinal and aromatic plants, and ornamentals

  • Over 19 million plants have so far been dispatched
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MTP

  • Large-scale multiplication of superior clones of

various species using tissue culture

  • Mass propagation of species that are difficult to

regenerate by conventional methods

  • Clonal fidelity of tissue cultured plants is

ensured through periodic testing by molecular markers

  • TC plants are further fortified with suitable

mycorrhiza for better establishment in the field and improved growth

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Media lab

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Autoclave room

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Autoclaving

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Inoculation Room

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Growth Room

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Greenhouse

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Greenhouse

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Polyhouse: Low-cost hardening solution

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Shadehouse

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Plants growing in the open nursery

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Populus deltoides Paulownia fortunei Leucaena hybrids Eucalyptus tereticornis Eucalyptus citriodora Eucalyptus camaldulensis Dendrocalamus strictus Dendrocalamus asper Bambusa nutans Bambusa balcoa Bambusa bambos Anogeissus pendula Anogeissus latifolia Forestry species Pineapple Strawberry Citrus (Rootstock) Banana Apple (Rootstock) Fruit Species Large cardamom Black pepper Turmeric Sugarcane Hops Cash Crops Potato Leek Ginger Asparagus Vegetable Crops Vanilla Swertia chirata Stevia Rauwolfia serpentina Patchouli Geranium Crataeva nurvala Chlorophytum borivilianum Centella asiatica Bacopa monnieri Asparagus racemosus Aloe Vera Medicinal & Aromatic Plants Ornamentals (foliage and flowering) Aglaonema Ficus Hibiscus Alpinia Miscanthus Pinguicula Calathea Nandiana Plumbago Dieffenbachia Philodendron Rose Dracaena Syngonium Thalictrum Drosera Yucca Verbascum

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Major clients

DOMSETIC MARKET

  • Forest & Horticulture departments
  • R&D projects supported by DBT, Govt. of

India

  • Agro-based companies
  • Private entrepreneurs
  • Nurseries/Individual farmers/growers

INTERNATIONAL MARKET Countries where plants are exported :

  • The Netherlands, Germany, South Africa &

Australia

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Fruit crops

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Strawberry

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Strawberry cultivation near Shillong

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Pineapple in Assam

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Citrus

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Apple (rootstock)

Shoot multiplication Rooting

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Shoot multiplication (Scion wood)

Scarlet Gala Vance Delocious

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Cash crops

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Potato

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Potato minitubers

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Potato cultivation at Supi

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Farmers’ meet in Uttarakhand

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Sugarcane

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Ginger

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Turmeric

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Black pepper

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Large cardamom

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Asparagus

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Medicinal plants

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Swertia chirata (Chirayta)

  • Endangered, annual herb

found in temperate climate at an altitude of 1,200 - 1,500 m

  • Prized as a tonic; Used in

chronic fevers, anemia, bronchial asthma, liver disorders, anti-malarial, anti- helminthic & other diseases

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a b c d

Swertia chirata

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Rauwolfia serpentina Picrorhiza

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Valeriana Chlorophytum

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Stevia rebaudiana

Multiplication Rooting

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Bacopa monnieri (Brahmi)

Shoot multiplication Rooting

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Forest species

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Bambusa balcooa

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Dendrocalamus asper

Shoot multiplication Rooting

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Foliage

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Representative ISSR fingerprint profiles of in vitro propagated plantlets of S. chirayita

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Services offered by MTP

  • Supply of tissue cultured plants of various species

in the domestic and the international markets

  • Contractual research/production for :
  • Development of micropropagation protocols

for new crops

  • Refining of existing protocols for making them

suitable for appropriate business needs

  • Mass production and supply of plants on an

exclusive basis

  • Technology transfer
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  • NO reinventing of wheel
  • NO ornamentals and species like banana, etc
  • Focus on :
  • Certified seed program (potato, ginger,

turmeric, etc)

  • Grafted crops (apple, citrus, etc)
  • Ecologically important crops
  • Endangered/threatened crops
  • Introduction and multiplication of exotic

germplasm of high potential

Role of government

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  • Multiplication of parental lines for hybrid seed

production

  • Early commercialization of newly bred lines
  • Multiplication of transgenics
  • Supply of virus-indexed mother cultures of

superior individuals

  • Transfer of proven technologies to the industry/

entrepreneurs

  • Setting-up of incubation centres

Role of government

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  • Impart training for large-scale production of

plants by tissue culture

  • Demonstration trials for testing technology and

creating awareness

  • Subsidy
  • Setting standards for TC companies

Role of government

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Other aspects

  • Selection
  • Chemoprofiling
  • Virus indexing
  • Clonal fidelity
  • Mycorrhiza
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