Micropropagation Technology Park Sanjay Saxena Micropropagation - PowerPoint PPT Presentation

Micropropagation Technology Park Sanjay Saxena

Micropropagation Technology Park at Gurgaon, Haryana

MTP • MTP is one of the largest tissue culture production units in north India with an annual production capacity of 1.5 million plants • The facility is ISO 9001 certified and DBT, Govt. of India accredited reflecting the quality control and assurance of the lab • Micropropagation protocols have been developed for over 90 species including, forestry species, fruit crops, cash crops, medicinal and aromatic plants, and ornamentals • Over 19 million plants have so far been dispatched

MTP • Large-scale multiplication of superior clones of various species using tissue culture • Mass propagation of species that are difficult to regenerate by conventional methods • Clonal fidelity of tissue cultured plants is ensured through periodic testing by molecular markers • TC plants are further fortified with suitable mycorrhiza for better establishment in the field and improved growth

Media lab

Autoclave room

Autoclaving

Inoculation Room

Growth Room

Greenhouse

Greenhouse

Polyhouse: Low-cost hardening solution

Shadehouse

Plants growing in the open nursery

Forestry species Fruit Species Medicinal & Aromatic Plants Anogeissus latifolia Aloe Vera Apple (Rootstock) Anogeissus pendula Asparagus racemosus Banana Bambusa bambos Bacopa monnieri Citrus (Rootstock) Bambusa balcoa Centella asiatica Strawberry Bambusa nutans Chlorophytum borivilianum Pineapple Dendrocalamus asper Crataeva nurvala Cash Crops Dendrocalamus strictus Geranium Hops Eucalyptus camaldulensis Patchouli Sugarcane Eucalyptus citriodora Rauwolfia serpentina Turmeric Eucalyptus tereticornis Stevia Black pepper Leucaena hybrids Swertia chirata Large cardamom Paulownia fortunei Vanilla Populus deltoides Ornamentals (foliage and flowering) Vegetable Crops Aglaonema Ficus Hibiscus Alpinia Miscanthus Pinguicula Asparagus Calathea Nandiana Plumbago Ginger Dieffenbachia Philodendron Rose Leek Dracaena Syngonium Thalictrum Potato Drosera Yucca Verbascum

Major clients DOMSETIC MARKET • Forest & Horticulture departments • R&D projects supported by DBT, Govt. of India • Agro-based companies • Private entrepreneurs • Nurseries/Individual farmers/growers INTERNATIONAL MARKET Countries where plants are exported : • The Netherlands, Germany, South Africa & Australia

Fruit crops

Strawberry

Strawberry cultivation near Shillong

Pineapple in Assam

Citrus

Apple (rootstock) Shoot multiplication Rooting

Shoot multiplication (Scion wood) Scarlet Gala Vance Delocious

Cash crops

Potato

Potato minitubers

Potato cultivation at Supi

Farmers’ meet in Uttarakhand

Sugarcane

Ginger

Turmeric

Black pepper

Large cardamom

Asparagus

Medicinal plants

Swertia chirata (Chirayta) • Endangered, annual herb found in temperate climate at an altitude of 1,200 - 1,500 m • Prized as a tonic; Used in chronic fevers, anemia, bronchial asthma, liver disorders, anti-malarial, anti- helminthic & other diseases

a b c d Swertia chirata

Picrorhiza Rauwolfia serpentina

Valeriana Chlorophytum

Stevia rebaudiana Multiplication Rooting

Bacopa monnieri (Brahmi) Shoot multiplication Rooting

Forest species

Bambusa balcooa

Dendrocalamus asper Shoot multiplication Rooting

Foliage

Representative ISSR fingerprint profiles of in vitro propagated plantlets of S. chirayita

Services offered by MTP • Supply of tissue cultured plants of various species in the domestic and the international markets • Contractual research/production for : - Development of micropropagation protocols for new crops - Refining of existing protocols for making them suitable for appropriate business needs - Mass production and supply of plants on an exclusive basis • Technology transfer

Role of government • NO reinventing of wheel • NO ornamentals and species like banana, etc • Focus on : -Certified seed program (potato, ginger, turmeric, etc) -Grafted crops (apple, citrus, etc) -Ecologically important crops -Endangered/threatened crops - Introduction and multiplication of exotic germplasm of high potential

Role of government • Multiplication of parental lines for hybrid seed production • Early commercialization of newly bred lines • Multiplication of transgenics • Supply of virus-indexed mother cultures of superior individuals • Transfer of proven technologies to the industry/ entrepreneurs • Setting-up of incubation centres

Role of government • Impart training for large-scale production of plants by tissue culture • Demonstration trials for testing technology and creating awareness • Subsidy • Setting standards for TC companies

Other aspects • Selection • Chemoprofiling • Virus indexing • Clonal fidelity • Mycorrhiza