Microbiome research in freshwaters Jason Woodhouse and Hans-Peter - PowerPoint PPT Presentation

Microbiome research in freshwaters Jason Woodhouse and Hans-Peter Grossart Department of Experimental Limnology, Leibniz Institute of Freshwater Ecology and Inland Fisheries, Stechlin, Germany

Freshwaters Source: Igor Shiklomanov's chapter "World fresh water resources" in Peter H. Gleick (editor), 1993, Water in Crisis: A Guide to the World's Fresh Water Resources (Oxford University Press, New York).

Freshwaters

Freshwaters • Drinking water quality • Eutrophication impacts • Climate and weather impacts • Carbon cycling

Defining the freshwater microbiome • Who is there? • What are they doing? • Who is doing what? • How does this change over time/space

Who is there? • Viruses • Bacteria • Protozoa • Fungi • Phytoplankton • Zooplankton • Other eukaryotes (e.g. Oomycetes)

Who is there? - Heterotrophs Newton et al. Microbiol. Mol. Biol. Rev. 2011; doi:10.1128/ MMBR.00028-10

Who is there? – Heterotrophs Woodhouse et al. ISMEJ 2016

Who is there? – Aquatic Fungi Grossart et al. Nat. Rev. 2019; doi:10.1038/s41579-019-0175-8

Who is there? – Aquatic Fungi Perkins et al. Fungal Ecology 2019; doi:10.1016/j.funeco.2019.04.004 Wurzbacher et al. MycoKeys 2016; doi:10.3897/mycokeys.16.9646

Is this accurate? Ionescu et al. Nat. Comm. 2017; doi:10.1038/s41467-017-00342-9

Is this accurate? Ionescu et al. Nat. Comm. 2017; doi:10.1038/s41467-017-00342-9

What are they doing – Oxic Methane Bizic-Ionescu et al. bioRxiv 2019; doi:10.1101/398958 Grossart et al. bioRxiv 2011; doi:10.1073/pnas.1110716108

What are they doing – Oxic Methane

What are they doing? – Organic Matter

Phytoplankton-Bacteria Interactions

Microbe-Activity Linkage (Long-Term)

Microbe-Activity Linkage (Long-Term) • What does the future hold? • Will freshwater systems continue as sources of GHG emissions? • Will eutrophication/warming • Can we begin to predict how lakes will evolve or whether they will stay the same? • Can/Should we intervene to “save” lakes?

Take home messages • Freshwaters contain discrete and ever changing microbial populations • Applications of modern sequences approaches should be considered carefully with respect to new and traditional microbiological and biogeochemical methods • Microbiome research in freshwater systems allows us to generate hypotheses applicable also to marine and terrestrial systems • Freshwater microbiomes are critical components of the whole earth system

Thank You!!