Material Purpose Characters Critical Indigenous, Exotic, Acquisition Morphological Weather, Heading dates COLLECT NERICA,DRR, Climate Model Phenological based on sowing, HEAT CRRI,IGKVV, Development Agronomic Stomatal, LT, Resp, Stigma receptivity, pollen PAU,IRRI,NBPGR.IRH Responsive fertility, grain filling, TNVar.CMS,Res. Yield relative yield Microbes Based on FS Identification Leaf Temp & Stomatal, Pn, SELECT Florescence, LT NUE DRR / Multi Traits Climate Rolling Photoresp Adaptation MSI,ROS Sec.branches.pan F1,Hybrids Evaluation for SSR/QTL/ Transcription, Translation BREED Backcross, BC1F1, Climate Candidate Expression at phenotype GXE BC2F2, Mapping pop Resilience Gene map Integrating crop & climate Development models LINKAGES Rqd: CRIDA, CCMB, UoH for Phenomics,FATE anatomical works etc.,
Progress made Upto June,2011-Plant Breeding & Hybrid rice Procurement : NERICA 53 HTL 28 Wild: PAU, IGKV, CRRI 163 lines of IRHTN,CPAU &HTL,400 lines of Germplasm,200 lines of Basmati &ASG,50 lines each DRR & Upland and hill Two and three dates of sowing Screening : 168 lines (55 lines IRHTN, 2010,33 IRHTN 2009,75lines HTL,PAU,CPAU 62 Restorer and maintainer lines Relatively tolerant lines IR 20,N22, Balila,CR-547-1-2-3,IR2307-247-2- 2-3 Sensitive: Sadri,GZ 948-2-2-1,Zandrome, IR50,Mulai 7 crosses, sensitive to sensitive and 3 (2) sensitive and tolerant (1) 10 promising IRHTN 15 already reported HT lines used in crossing program with CMS . Phenotype for: Plant Height, EBT,Panicle Length,Grain No/pan, Grain yield,Initial and 50% Flowering,% Chaff, quality were done.
Dr N.Shobharani, LVSubbarao, VP Bhadana, Lal, Bush and Sudheer Senguttivelu, Sravan raju
Table 1: Means table (nine genotypes) of rice genotypes under normal and late sown conditions during rabi 2010. Character Normal Late Sowing Genotyp S x G (s) es (G) Shoot wt.m-2 (g) 581 562 - 119** - TDM m-2 (g) 1277 1278 - 163** - Panicle no 454 402 48.7** 104** 146** Panicle wt (g) 696 717 98.7** - - Spikelet x 103 55 53 - 13** 18** Spikelets.pan 86 144 54.2** 27.8** 39.4** Grain no x 103 37 36 - 7.6** 8.1** Chaff no x 103 18 16 - 9.4** 9.9** Grain no.pan 129 98 68.5* 39.8** 56.3** Grain wt (g) 606 650 - 113** - Chaff wt (g) 51.7 47.4 _ 28** - 1000 grain wt (g) 17.06 18.37 1.19* 1.52** 2.14** HI (%) 47.56 50.66 1.41* 3.83** -
Control Heat tunnel Parameter High Low High Low Water -2.80 -5.80 -2.70 -6.80 Potential (mpa) OP (Mpa) -1.47 -1.96 -1.38 -2.1 % 56 17.8 70 18 Translocatio n Spikelet 93 64 70 15 fertility Pollen 100 89 65 56 viability Grain yield 937 503 423 117 Table: Terminal heat stress on some physiological attributes: 25 rice cultures
Photosynthesis Leaf temperature Phenotyped: Leaf rolling, thickness, temperature, LAI, Tiller no Leaf no, FV/FM, Membrane stability, Spad Values, FV/FM, under three dates of sowing 62 lines, (hybrids,CMS,Parental lines), 22 lines of Physiology experimental fields (heat tolerance) and also for 15 genotypes (No andN100 levels) at field level Screening 1 st cycle carried. Methods for leaf surface prints standardized.
Adaxial leaf surface showing trichomes Conserve heat flux during dry weather PA-6129
Cellulose acetate butyrate peelings of Trichomes
Azucena BPT 5204 06 13 Relative heat tolerance in Azucena (land race) may be related to stomatal no. per unit area . IR-64 Jaya 08 14 No.of Stomata crucial for determining heat tolerance
Stomatal index 26 RPHR-1005 22 24 23 22 27 RPHR-1096 15 22 22 18 S.No Taxa Stomatal No. Stomatal size (µm) 28 SC5-2-2-1 18 23.5 25 20 Upper Lower Length Width 29 20 26 22 17 1 Ajaya 18 28.5 23 20 30 SG-27-77 20 27 21 15 2 CORH-3 27.5 29.5 20 17 31 SG-27-175 17.5 18 21 18 3 DRRH 2 17.5 17 20 19 32 111-3 21 18.5 23 15 4 DRRH 3 29.5 22.5 24 19 33 517 25 26.5 22 18 5 JKRH 401 29.5 32.5 18 17 34 611-1 18 24 20 17 6 JRH 8 26.5 27.5 18 14 35 619-2 20 27 20 16 7 KRH 2 18.5 26 14 16 36 APMS 6B 16.5 25.5 20 18 8 NK 5251 34.5 37 22 17 37 IR 58025B 5 4.5 34 24 9 PA 6129 26 28 20 14 38 IR 68897B 23 30 20 16 10 PHB 71 24 29 20 16 39 IR 79156B 11 PRH 10 25 26.5 18 18 40 PUSA 5B 26 29 19 20 12 Rajalaxmi 24 27 23 20 41 IR 68888B 15 22 24 18 13 Sahyadri -4 18 24 20 18 42 DRR 6B 19 22 22 16 14 Suruchi 18 26 22 18 43 DRR 9B 14 18 23 16 15 US-312 23 25 23 23 44 DRR 10B 19 23 22 18 16 BCW-56 19 26 23 17 45 DRR 3B 22 25 20 17 17 46 APMS 6A 16 17.5 20 19 18 DR714-1-2-R 27 30 21 15 47 IR 58025A 18 36 22 15 19 EPLT-104 21 27 17 16 48 IR 68897A 17 17 23 15 20 EPLT-109 20 29 19 19 49 IR 79156A 17 20 18 12 21 GQ-25 25 28 19 16 50 PUSA 5A 22 27 22 15 22 IBL-57 23 26 22 18 51 IR 68888A 18 23 21 18 23 IR 40750R 17 23 23 21 52 DRR 6A 18 22 20 18 24 KMR3 22 30 19 16 53 DRR 9A 17 23 23 14 25 RPHR-1004 24 26 20 17 54 DRR10A 12 12 28 17
Leaf rolling Varieties Leaf Rolling N-100 N-0 Rasi 2.6 2.59 Anjali 2.97 2.9 Annada 2.623 2.62 Prassanna 2.196 2.1 SR Voleti, D.Subrahmanyam and PR RAO MTU1010 2.173 2.17 Varadhan 2.796 2.76 Jaya 2.603 2.59 Sampada 2.473 2.45 KN.Swamy, K.Rajesh, B.sailaja, PHB 71 2.696 2.67 Vijayalakshmi,Y.V Rao and T.V.Kiran Pusabasmati 1 2.15 2.11 Vasumathi 3.24 3.21 Swarna 2.34 2.33 BPT 5204 1.08 1.07 DRRH2 2.7 2.71 Mahasuri 2.68 2.6
Leaf thickness Leaf area Varieties Leaf thickness Varieties Total Leaf Area N-100 N-0 N-100 N-0 Rasi Rasi 0.17 0.24 20.65 25.76 Anjali Anjali 0.173333333 0.27 21.15 14.63 Annada 0.233333333 0.226666667 Annada 14.1 16.86 Prassanna 0.24 0.246666667 Prassanna 12.18 12.48 MTU1010 0.223333333 0.22 MTU1010 15.75 12.12 Varadhan Varadhan 0.22 0.196666667 17.6 13.92 Jaya Jaya 0.233333333 0.203333333 25.84 12.88 Sampada Sampada 0.236666667 0.213333333 17.29 9.1 PHB 71 PHB 71 0.23 0.233333333 17.92 20.4 Pusabasmati 1 Pusabasmati 1 0.283333333 0.246666667 15 16.03 Vasumathi 0.276666667 0.22 Vasumathi 16.98 12 Swarna 0.24 0.183333333 Swarna 12.66 9.5 BPT 5204 0.223333333 0.203333333 BPT 5204 10.98 12.9 DRRH2 DRRH2 0.253333333 0.22 13.56 10.08 Mahasuri Mahasuri 0.246666667 0.16 19.6 8.88
Respiration Methodology: Leaf sample was collected freshly and kept in cold conditions S.No Variety mg of CO 2 released/gm dry matter 100 mg of leaf was weighed into a 20 ml test tube and to that 1 ml of 1% of sodium hypochlorite was taken 1 HS1 921.97 All the experiment was done in dark to prevent 2 HS2 1150.58 further photosynthesis in leaf. 3 HS3 1047.96 30 ml of NaoH (0.35M) was taken into 75 ml test tube. 4 HS4 1063.55 The 20 ml test tube containing leaf sample was kept into 5 HS5 1091.18 75 ml test tube and it was sealed air tight with parafilm and aluminium foil. 6 HS6 1351.68 The test tubes were incubated for 3 days in dark conditions 7 HS15 305.32 After incubation 2 ml of 0.5M Bacl2 was added to NaoH 8 HS18 237.96 to precipitate absorbed CO2 as BaCo3 9 HS19 136.00 Then 3 to 4 drops of phenolpthaleine was added and 10 HS25 178.42 titrated against 0.3M Hcl to estimate remaining NaoH
The decrease in chl a/b ratio is less in NH219 than N-22, which indicates tolerance to stress induced senescence Biotech Group: Methods for stigma Receptivity, pollen Fertility, in vitro germination N. Sarla, D.V.N Chakravarthi (RA), V. Vishnu Prasanth (SRF) Field experiment, RB 2011
NH219 mutant is heat tolerant based on KI staining of pollen Control Heat Stigma receptivity IR64 N-22 NH219
Genotype response to in vitro pollen germination in modified liquid medium at room temperature Genotype % Germination Swarna 16.03 ± 1.6 4.33 ± 0.05 KMR3 7.42 ± 0.44 IR66R Microscopic view of pollen 55.83 ± 0.41 IR64 germinated in vitro a. in medium Utrirajapan 85.80 ± 4.35 of Shaojun et al. (2006). BPT5204 34.83 ± 0.76 b-d. in modified medium b.Utrirajapan
Field view at harvest
Nitrogen Use efficient rice cultivars Highly eff. Medium eff. Low eff. Varadhan MTU 1010 Prasanna PA 6444 Swarna Vasumati Rasi Sampada Mahsuri Jaya DRRH 1 BPT 5204 Annada Pusa Basmati 1 Anjali Field screening of 15 rice genotypes for Nitrogen use efficient undertaken and grouped. Identification of genes and QTL related to NUE is initiated
Nitogen use efficiency indices Var. AE PE RE IUE PFP NHI (%) Kg Kg Nup/N Yld/To Yld/ N Gr. Nup gr/kg N gr/kg N added t N up added /tot Nup up 5.4-26.2 15-37 36-70 43-53 37-59 0.64-0.76 Early (15.8) (26.4) (55.4) (48.2) (49.4) (0.67) (5) 10-24.9 22-46 32-83 38-60 43-72 0.56-0.7 Med. (17.1) (31.6) (54.4) (52.0) (57.7) (0.66) (7) 7.5-18.1 18-29 42-71 38-44 48-52 0.57-0.62 Late (12.9) (21.7) (58.0) (41.0) (50.3) (0.59) (3) Figures in brackets are mean values � Most of the NUE indices were higher in case of medium group var. followed by early group
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