lium Cyst stic icer ercosis in sis in West ester ern Ken enya - - PowerPoint PPT Presentation

Subtitile

Taen enia ia so soli lium Cyst stic icer ercosis in sis in West ester ern Ken enya

Lian Do Doble le

Overvie iew

• People, Animals and their Zoonoses
• Preliminary Results
• Regional Picture
• Food Chain Risk Analysis
• Pen-side diagnostic development
• Free-range pig ecology
• Future development of this work

People le, Anim imals ls & their ir Zoonose ses

• Cross-sectional survey in

Western Kenya

• Multi-species
• Multi-pathogen
• Inter-disciplinary
• Collaborative
• 413 comprehensive

homestead surveys

• 370 homes visited thus

far

• 1782 people
• 904 cattle
• 247 goats
• 81 pigs

Slaug laughter Sla lab Sur urvey

• 343 pigs sampled
• March – September

2010

• Licensed slabs
• Lingual Palpation
• Serology using

HP10 Ag-ELISA

Prelim limina inary Result lts

• Raw data presented at meeting removed

– publications to follow later this year!

Region ional Coll

• llabor

borations ions (da data to be be pu publis blished d later)

• Homa Bay (Kenya)
• University of Nairobi
• Farm based, multi-stage, random selection of

villages

• All pig-keeping homes within selected villages

sampled

• 232 samples tested with HP10 Ag-ELISA
• Lake Kyoga (Uganda)
• Makerere University
• Farm based, multi-stage random selection of sub-

counties

• House-to-house vists until minimum sample size

per sub-county achieved

• 378 samples tested with HP10 Ag-ELISA

Food Chai ain Risk isk Asse ssessm sment

• Determine the risk of any single

pork meal eaten in Western Kenya being infectious with T.solium

Model l Struct uctur ure

Risk sk pig ig is is Infect cted wit ith T.so solium lium

• Data from PAZ slaughter slab survey
• 48% Pigs positive on HP10 Ag-ELISA
• Indicates pigs with viable cysts
• Literature suggests no significant difference in prevalence

between formal and informal slaughtered pigs

If pig pig is is inf infected; d; what is is the pr prob

• babilit

bility of

• f any

ny one

• ne meal

l cont

• ntainin

ining cystic icerci?

• Based on experimental

infection data (Sciutto et al., 1998 & Deckers et al., 2008)

• Level of infection of pig
• Dissection and

numeration of cysts

• Beta distribution for

proportion

• Proportion of meals

infected in different infection levels

• no. cysts/ 2kg pork
• Average meal size of

0.25kg (assumption)

• Uniformly distributed as

little prior knowledge

Is s the pork k well ll co cooke ked?

• PAZ homestead survey data
• Any response bar ‘Brown throughout’ or ‘Fully

Roasted’ = potential for undercooked (17%)

• Beta distribution

Model l Out utput ut: what at is is the cur current st stat ate of af affair irs? s?

• Data removed pending publication

What at ca can be done?

Pen-si side diag iagnost stic ic developments

Benefits:  Relatively cheap & quick  Little training needed  Currently in development (ILRI/UoE) Barriers:  Requires incentive for use

How might this is tool l be use used in co control?

Fr Free-Ranging ing Pig ig Ecolog

• logy St

Study dy

• 10 randomly

selected pigs

• Data removed

pending publication

Summar ary

• T.solium endemic in Western Kenya and

areas of Uganda

• Very high number of infective pigs

slaughtered

• Huge potential for infective meat to

reach consumer

• Several interventions have potential to

reduce this risk

• Exclusion of infective pigs using new

diagnostic test is realistic and achievable

Which ich way ay now?

• Finish laboratory diagnostics
• Analysis of co-infection data and risk factors
• No-gold-standard analysis of pen-side test

THANK YOU THANK YOU

• Er

Eric ic Fevre (PI) Er Eric ic Fevre (PI)

• The

The ‘ ‘PAZ PAZ’ ’ team am team am

• Le

Lesl slie ie Har arriso ison Le Lesl slie ie Har arriso ison

• Pie

ierre Pie ierre Dorny Dorny & Sar arah ah & Sar arah ah Ga Gabrie iel Ga Gabrie iel

• Phil

il Toye ye Phil il Toye ye

• BBSRC

BBSRC

• Wellc

llcome Trust ust Wellc llcome Trust ust – – grant no. grant no. 085308

085308

• ASARECA

ASARECA

And thank k yo you u for list listening! And thank k yo you u for list listening!