LGM International Inc., Ft. Lauderdale Second Generation of Liquid- - - PowerPoint PPT Presentation

Second Generation of Liquid Second Generation of Liquid-

• Based Cytology

Based Cytology

LGM International Inc., Ft. Lauderdale

▪ All Cells collected (100%) are preserved for analysis. ▪ Preservation & fixation of cells is immediate better preserving detailed nuclear morphology. ▪ Remove the blood and mucus, and clinically relevant cells are no longer occluded from field of vision (hidden cell). ▪ With clearer field of vision, slides can be scanned with greater accuracy (missed cell). “LBC Marketing Myth” ▪ HOWEVER, LBC is only as accurate as the cytologist who reads it!!!! ▪ LBC is a new tool for qualified cytologists to read with greater accuracy.

What makes LBC technology superior ?? What makes LBC technology superior ??

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Liqui-PREPTM System

Liqui Liqui-

• PREP

PREPTM

TM Second Generation LBC

Second Generation LBC

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Liqui-PREPTM System

LBC Products LBC Products – – Market Evolution Market Evolution

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First Generation First Generation Second Generation Second Generation High cost pert test. Special Equipment (capital funds). Change in cellular morphology. Specialized training to read. Utilize only portion of specimen. Bloody specimens/clogged filters or acid pre-treatment. HAZMAT disposal costs. What is missed in residual vial? Low cost per test. Standard centrifuge, vortex & pipette. Classic morphology. Utilizes whole specimen. No filters to clog. Separation chemistry to clean specimens. Non-HAZMAT formulations. Designed for non-gyn as well.

Liqui-PREPTM System

Collection

Collection Choices

Endocervix brush All in One Combi Brush Cervix Brush

Preservative Vial Features

• Alcohol based- preserving standard

cellular morphology

• Non-Hazmat – no exposure risk, lower

shipping and disposal costs

• Detachable brush head – assures all

cells collected are fixed for analysis

• Room temperature storage
• Preserves cells, bacteria, protozoan,

fungi, etc. for microscopic analysis

• Protects and preserves nucleic acids and

proteins allowing for subsequent follow-on testing, i.e. HPV, Chlamydia, etc.

Cleaning Solution

ALL cells collected are poured onto the Cleaning Solution Features:

► Whole specimen is processed ►Utilizes separation chemistry to remove mucus ►Lytic Preservative lyses red blood cells ►Clinically relevant cells travel to bottom ►Bacteria, yeast, fungi, protozoan are preserved and captured in cell pellet

1,000g force-10 minutes

Slide Preparation

Cell Base encapsulates cells, nucleic acids and proteins Cell Base provides: ►adhesion to slide

►forms thin layer ►porous for rapid staining ►adjustable for various cell pellets

►set your own cell concentration

Cell Base* Chemistry

Encapsulation of cells creates a density gradient for heavier cells to settle and cells seek space within the matrix

* Patents Pending

Proprietary compound forms an adhesive seal to clean standard microscope slides, avoiding expensive slide pretreatment

Classic morphology, i.e. honeycomb, picket-fence, lesion aggregation/clumps, are all maintained in the matrix, not broken up by filters

As matrix dries, pores form within the matrix to allow stains, IHC to react on cell surfaces Optimized Cell Density: No two specimens are alike, cell density/cell pellet ratio will impact final cell density on the slide. Cell Base can be

• ptimized in a 1:2 to 1:8 ratio based upon cells collected to achieve the

desired cell density, unlike a automated processor which dilutes all specimens the same.

Graduated Tube (ul)

Benefits of LBC Benefits of LBC LBC (by Liqui-PREPTM) Conventional Pap Smear

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Liqui-PREPTM System

Sample Preps

Endocervical Cells Negative within normal limits

Sample Preps

Benign cellular changes associated with Herpes infection Benign cellular changes associated with Candida albicans

Sample Preps

Squamous cell - ASCUS Squamous Cell - LSIL

Sample Preps

Squamous Cell- HSIL Squamous cell Carcinoma

Sample Preps

Endocervical adenocarcinoma Thyroid

Better Solutions through Better Chemistry!