Lam ia Krichen I ncom ing Agreenskills fellow ( Septem ber 2 0 1 4 - - PowerPoint PPT Presentation
Lam ia Krichen I ncom ing Agreenskills fellow ( Septem ber 2 0 1 4 - August 2 0 1 5 ) Laboratory of m olecular genetics, im m unology and biotechnology, Faculty of Sciences of Tunis, University of Tunis-El Manar ( UTM) , Tunisia Hosting team
Hosting team : AFEF ( Architecture et Fonctionnem ent des Espèces Fruitières) -UMR AGAP ( Am élioration Génétique et Adaptation des Plantes) - CI RAD-SupAgro Montpellier, France
I ncom ing Agreenskills fellow ( Septem ber 2 0 1 4 - August 2 0 1 5 ) Laboratory of m olecular genetics, im m unology and biotechnology, Faculty of Sciences of Tunis, University of Tunis-El Manar ( UTM) , Tunisia
(Prunus
unus arm eniaca L.) in Tunisia and their relationship with the
Mediterranean variability”. a jointly supervised work between the Laboratory of Molecular Genetics I mmunology and Biotechnology of the Faculty of Sciences of Tunis and SI BAGHE doctoral school of SupAgro Montpellier agriculture school in collaboration with I NRA Avignon.
“ Morphological characterisation of Tunisian apricots ” 2 0 0 7 -2 0 1 4 : University habilitation “ Prunus
unus ar arm eniac aca L.:
Conservation strategy and dissem ination history”
High I nstitut of Biological Sciences of Tunis ( I SSBAT) - University of Tunis-el Manar
Faculty of Sicences of Gafsa – University of Gafsa
courses, practical w ork and directed w ork
reproductive biology in plants, and on im provem ent and conservation of plant resources
Tunisian apricot diversity w ith the Mediterranean variability
Mediterranean Basin
Objective: Analyze the differencially expressed genes under water stress and well watered conditions in order to identify candidate genes for tree responses and adaptation to soil water restriction.
Motivation for this postdoctoral study is that the water stress aspect in fruit species is a very relevant topic for Tunisia. This topic is a continuity of acquired genetic diversity and its use in plant breeding
Part 1: Analyze dataset obtained from the AryANE micro-array apple chip to identify ontologies and metabolic pathways differentially expressed between stressed and well watered trees
Part 2: Selection of candidate genes implied in tree adaptive responses
■ Apex, leaf and root sam ples w ere collected from
plants growing in soil water restriction (two levels of water restriction: 50% and 5% of the fraction
with well watered plants ■ RNA extraction from Apex, leaf and root ■ AryANE m icro-array apple chip analyzed in UMR I RHS- I NRA ANGERS
▪ Differentially expressed genes (up and down regulated) between water stressed and well watered samples of apex, leaf and root from two apple cultivars
Data filtering and Venn Diagram of differentially expressed genes
e.g. Gene
▪ Genes are annotated to main biological functions with associated Gene
terms (AgriGO)
▪ Genes are assigned to m olecular pathw ays based on the Arabidopsis thaliana and the Rosaceae Databases ( Mapm an softw are)
( Yue et al. 2 0 1 2 )
genes im plied in abscisic acid. synthesis- degradation.synthesis.9 -cis-epoxycarotenoid dioxygenase ( Hao et al. 2 0 0 9 )
( Fujita et al. 2 0 0 5 )
responsive-activated ( Tabata et al. 2 0 0 0 )
Analyse the specific expression of the identified candidate genes resulting from the confrontation between QTL zones and transcriptomic data
Explore the different forms for each of them in the apple genome; to define specific primers Perform quantitative RT-PCR for confirming the differential expression of a limited number of these genes in an independent experiment
several fields of research such as RNA extraction, PCR and RT-PCR, bioinformatics to define primers and analyses micro-array data set
my contacts through participation to international conferences and meetings
level