IRENESERRANO Team: Dominique Roby Supervisor: Susana Rivas AgreenSkillsProject Deciphering the protective roles of transcriptional regulators of innate immunity during seed development in Arabidopsis thaliana
Personal Background 2006 Master in Agricultural Sciences (University of Granada, Spain) 2010 PhD. with European Honors (Estacion Experimental del Zaidin/University of Granada, Spain) Mechanisms of incompatible pollen rejection in olive tree (Olea europaea L.) Visiting scholar: - IRD, Montpellier (France). - MTA-AKT, Martonvasar (Hungary). - IPGB, Nitra (Slovakia). - EMBRAPA, Brasilia (Brasil). - IVIA, Valencia (Spain). 2010-2015 Postdoctoral Fellow/Research Assistant (Indiana University, Indiana, USA) Control of PCD and disease resistance by EDR1 2015- AgreenSkills Incoming Fellow (Laboratoire des Interactions Plantes- Microorganismes, Toulouse , France) Protective roles of immune regulators during seed development
Introduction The Hypersensitive Response Pathogen perception Signal transduction ? Transcriptional control Nucleus Cytoplasm target genes HR Resistance
Introduction MYB30 is a positive regulator of HR 1,5 Relative expression MYB30 + Pst 1 DC3000 0,5 AvrRpm1 0 hpi 0 1 2 3 4 5 MYB30 is specifically and transiently expressed after inoculation WT MYB30 OE MYB30 + Pst DC3000 AvrRpm1 Enhanced HR Nucleus 6 Cytoplasm * Log CFU/cm² target 5 + Pst ge genes 0 dpi DC3000 3 dpi 4 AvrRpm1 3 MYB30 OE WT MYB30OE HR HR Resistanc Resistanc Enhanced resistance e Vailleau et al., 2002
4 Introd Introduc uctio ion n MYB30 MYB30 regula regulate tes the s the VLCFA VLCFA biosyn biosynth thesi sis Affymetrix ATH1 « Whole genome » array WT MYB30 OE MYB30 AS MYB30 regulates VLCFA biosynthesis Raffaele et al. , 2008 Malonyl-CoA (C2) MYB30 C16:0-CoA C18:0-CoA HCD1 Acyl-CoA CER10 Elongase FDH complex KCS1 PAS2 KCS2 GL8 Nucleus Cytoplasm VLCF VLCFAs Cuticle VL VLCFA gen genes Wax esters Ketones Sphingolipids PM HR HR Resistanc Resistanc Cuticle PM Cell Death e Adapted from Raffaele et al. , 2009
Introd Introduc uctio ion n MYB30 is MYB30 is tightly tightly regulat regulated MYB30 MYB30 MIEL1 MYB30 sPLA2- α Ub Ub Ub Ub MIEL1 is an active E3 ligase and ubiquitinates MYB30 in vitro Nucleus MIEL1 leads to MYB30 proteasomal degradation in planta Cytoplasm VLCFA gen VL genes HR HR Resistanc Resistanc e
Introd Introduc uctio ion n A A role role for for MYB30 and MYB30 and MIEL1 in MIEL1 in the the seed ? seed ? MYB30 Cuticle Cuticle VLCF CFAs As The VLCFAs can also become cuticle components RNA Seq MYB30 MIEL1 30 MYB30 25 Cuticle formation starts at 20 rpkm the heart stage during 15 embryo developement 10 5 0 Globular Heart MYB30 and MIEL1 are expressed in the embryo bar.utoronto.ca/efp C. Balagué; unpublished
Object Objectiv ives s of my of my AgreenS AgreenSkil ills ls project project Do MYB30 and MIEL1 play a role in cuticle formation? MYB30 MIELs Cuticle default ? Toluidine Blue staining Cuticle composition ? GC-MS Do MYB30 and MIEL1 play a role in the defense of the embryo? MYB30 MIEL1 Vertical transmission Do MYB30 and MIEL1 play a role in the defense of the embryo? Meta-analysis of defence- and early embryogenesis- related transcriptomic datasets
First results First results MYB30 could have MYB30 could have a a role role in in embryo embryo cuticl cuticle format e formatio ion Seedlings are grown in the dark for three days Toluidin blue staining
First results First results MYB30 could MYB30 could be be crucia crucial l for for preven preventi ting pathog g pathogen en trans transmi miss ssion on through through the the seed seed F Flowering plants were spray inoculated with Xcc 568 and samples were F harvested 13dpi B F 1,00E+06 1,00E+06 Col-0 D0 Col-0 D0 1,00E+05 1,00E+05 Col-0 D13 Col-0 D13 1,00E+04 1,00E+04 myb30 D0 myb30 D0 1,00E+03 myb30 D13 1,00E+03 myb30 D13 MYB30OX D0 MIEL1OX D0 1,00E+02 1,00E+02 MYB30OX MIEL1OX D13 D13 1,00E+01 1,00E+01 miel1 D0 miel1D0 1,00E+00 1,00E+00 Buds Flowers
GROUP MEMBERS -Dominique Roby - Rémi Peyraud - Susana Rivas - Remy de Marchi - Corinne Audran - Vincen Duplan - Sylvain Raffaelle - Pierre Buscaill - Adelin Barbacci - Lucas Marmiese - Thomas Badet - Carine Huard-Chauveau - Marielle Barascud - Mehdi Khafif - Ullrich Dubiella - Malick Mbenge DÉVELOPPEMENT DE LA GRAINE GROUP - Gwyneth Ingram - Audrey Creff
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