IRENESERRANO Team: Dominique Roby Supervisor: Susana Rivas - - PowerPoint PPT Presentation

AgreenSkillsProject Deciphering the protective roles

• f

transcriptional regulators

• f

innate immunity during seed development in Arabidopsis thaliana

IRENESERRANO

Team: Dominique Roby Supervisor: Susana Rivas

Personal Background

2006

Master in Agricultural Sciences (University of Granada, Spain)

2010

• PhD. with European Honors (Estacion Experimental del Zaidin/University of

Granada, Spain) Mechanisms of incompatible pollen rejection in olive tree (Olea europaea L.) Visiting scholar:

• IRD, Montpellier (France).
• MTA-AKT, Martonvasar (Hungary).
• IPGB, Nitra (Slovakia).
• EMBRAPA, Brasilia (Brasil).
• IVIA, Valencia (Spain).

2010-2015 Postdoctoral Fellow/Research Assistant (Indiana University, Indiana, USA) Control of PCD and disease resistance by EDR1 2015- AgreenSkills Incoming Fellow (Laboratoire des Interactions Plantes- Microorganismes, Toulouse , France) Protective roles of immune regulators during seed development

Nucleus Cytoplasm

Introduction The Hypersensitive Response

Pathogen perception Signal transduction

?

Resistance

HR

target genes

Transcriptional control

Introduction MYB30 is a positive regulator of HR

Nucleus Cytoplasm

Vailleau et al., 2002

+ Pst DC3000 AvrRpm1

MYB30 is specifically and transiently expressed after inoculation

WT MYB30OE + Pst DC3000 AvrRpm1

Enhanced HR

+ Pst DC3000 AvrRpm1

3 4 5 6 WT MYB30OE Log CFU/cm²

0 dpi 3 dpi

*

MYB30OE

Enhanced resistance

Resistanc Resistanc e

HR HR

target ge genes

MYB30

0,5 1 1,5 1 2 3 4 5

Relative expression

hpi

MYB30

Introd Introduc uctio ion n MYB30 MYB30 regula regulate tes the s the VLCFA VLCFA biosyn biosynth thesi sis

4

VL VLCFA gen genes

Nucleus Cytoplasm

MYB30OE MYB30AS WT

Raffaele et al., 2008

Affymetrix ATH1 « Whole genome » array

MYB30 regulates VLCFA biosynthesis

Resistanc Resistanc e

HR HR

MYB30

Malonyl-CoA (C2)

C16:0-CoA C18:0-CoA

VLCF VLCFAs

Wax esters Ketones Sphingolipids

Cuticle PM Cell Death

Acyl-CoA Elongase complex FDH CER10 HCD1 KCS1 PAS2 KCS2 GL8 Adapted from Raffaele et al., 2009

Cuticle PM

Introd Introduc uctio ion n MYB30 is MYB30 is tightly tightly regulat regulated

VL VLCFA gen genes

Nucleus Cytoplasm

Resistanc Resistanc e

HR HR

MYB30 sPLA2-α

MIEL1 is an active E3 ligase and ubiquitinates MYB30 in vitro MIEL1 leads to MYB30 proteasomal degradation in planta

Ub Ub Ub MIEL1 MYB30 MYB30 Ub

Introd Introduc uctio ion n A A role role for for MYB30 and MYB30 and MIEL1 in MIEL1 in the the seed ? seed ?

Cuticle

Cuticle formation starts at the heart stage during embryo developement

bar.utoronto.ca/efp

• C. Balagué; unpublished

VLCF CFAs As

MYB30

Cuticle

The VLCFAs can also become cuticle components MYB30 MYB30 and MIEL1 are expressed in the embryo MIEL1

5 10 15 20 25 30

Globular Heart

rpkm

MYB30

RNA Seq

Object Objectiv ives s of my

• f my AgreenS

AgreenSkil ills ls project project

Do MYB30 and MIEL1 play a role in cuticle formation?

Toluidine Blue staining Cuticle default ? Cuticle composition ? GC-MS

MYB30 MIELs

Do MYB30 and MIEL1 play a role in the defense of the embryo?

Vertical transmission

MYB30 MIEL1

Do MYB30 and MIEL1 play a role in the defense of the embryo?

Meta-analysis of defence- and early embryogenesis- related transcriptomic datasets

First results First results MYB30 could have MYB30 could have a a role role in in embryo embryo cuticl cuticle format e formatio ion

Toluidin blue staining Seedlings are grown in the dark for three days

First results First results MYB30 could MYB30 could be be crucia crucial l for for preven preventi ting pathog g pathogen en trans transmi miss ssion

• n through

through the the seed seed

1,00E+00 1,00E+01 1,00E+02 1,00E+03 1,00E+04 1,00E+05 1,00E+06 Buds Col-0 D0 Col-0 D13 myb30 D0 myb30 D13 MYB30OX D0 MYB30OX D13 miel1 D0 1,00E+00 1,00E+01 1,00E+02 1,00E+03 1,00E+04 1,00E+05 1,00E+06 Flowers Col-0 D0 Col-0 D13 myb30 D0 myb30 D13 MIEL1OX D0 MIEL1OX D13 miel1D0

Flowering plants were spray inoculated with Xcc 568 and samples were harvested 13dpi

F F F B

GROUP MEMBERS

• Dominique Roby
• Rémi Peyraud
• Susana Rivas
• Remy de Marchi
• Corinne Audran
• Vincen Duplan
• Sylvain Raffaelle
• Pierre Buscaill
• Adelin Barbacci
• Lucas Marmiese
• Thomas Badet
• Carine Huard-Chauveau
• Marielle Barascud
• Mehdi Khafif
• Ullrich Dubiella
• Malick Mbenge

DÉVELOPPEMENT DE LA GRAINE GROUP

• Gwyneth Ingram
• Audrey Creff