Introduction Introduction Soil is an important resource which - - PowerPoint PPT Presentation

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Introduction Introduction Soil is an important resource which - - PowerPoint PPT Presentation

May 21, 2023 19 likes •201 views

Introduction Introduction Soil is an important resource which supports a large number of ecosystem goods and services from which humankind benefits, being terrestrial environment with the largest biodiversity. The capacity of the soil to maintain

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Introduction Introduction

Soil is an important resource which supports a large number of ecosystem goods and services from which humankind benefits, being terrestrial environment with the largest biodiversity. The capacity of the soil to maintain key ecological functions is known as soil quality. Soil face a large range of challenges, like :

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Microbial Community in Soil Microbial Community in Soil

In soil the microbial community play an important role in:

  • decomposition and stabilization of organic matter
  • immobilization and mineralization of nutrients
  • plant growth and nutrient uptake.

One of the parameters used to assess the impact of land use in soil microbial One of the parameters used to assess the impact of land use in soil microbial community size, activity, composition and diversity is the soil microbial respiration, which can be used as indicators of soil fertility, which if drop indicates a decrease in soil quality and health. Only a small fraction of soil microorganisms can be identified by conventional methods, that is why functional diversity can be used to understand microbial community.

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Objective Objective

The objective of this study was to assess the results of reviewed papers and the importance and efficiency of the MicroResp method in determining the physiological profile of the microbial community.

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MicroResp Method MicroResp Method

A method which can be used to assess the soil microbial community is MicroResp. The deep-well plate is used to load soil used to load soil samples and carbon sources and the detection plate is a system which detect the evolved carbon dioxide using cresol as an.

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Metal Clamp Detection Plate Seal

MicroResp Method MicroResp Method

Metal Clamp Deepwell Plate

Fig 1. MicroResp Kit

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CO2 + H2O + HCO3

  • →

2CO3

  • + 3H+

CO2 + H2O + HCO3

  • →

2CO3

  • + 3H+

MicroResp Method MicroResp Method

Fig 2. MicroResp working principle

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Review Construction Method Review Construction Method

No. Keywords Total Results Results of interest 1 "Community level physiological profile" AND Microresp AND Soil 89 4 2 Microresp AND Soil AND Microbiota 72 4 3 Microresp AND Soil And "Functional diversity" 210 4 4 Microresp AND Soil AND Fertilizer 150 4 5 Microresp AND Soil AND Wheat 136 4

  • Tab. 1. Search algorithm and results
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Review Construction Method Review Construction Method

Categories

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Results Obtained Using MicroResp Results Obtained Using MicroResp

Analysis of interactions between CLPP and fertilizers

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Results Obtained Using MicroResp Results Obtained Using MicroResp

Analysis of interactions between CLPP and pollution

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Results Obtained Using MicroResp Results Obtained Using MicroResp

Analysis of interactions between CLPP and crops

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Results Obtained Using MicroResp Results Obtained Using MicroResp

Analysis of CLPP in forest soils

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Results Obtained Using MicroResp Results Obtained Using MicroResp

Other studies to understand CLPP in soil

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MicroResp in Romania MicroResp in Romania

In Romania MicroResp method is just at the beginning but there are PhD theses which used this method to determine community level physiological profile.

  • Valentina SANDOR – Assesing the impact of edaphic biological communities
  • n biochemical processes in the soil to energy plants.
  • Susana SFECHIS - Researches influence of zeolite on productivity elements
  • Susana SFECHIS - Researches influence of zeolite on productivity elements

and microbiological activity on spring barley, soybeans and maize at ARDS Turda.

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MicroResp is a method which can be used in range studies for understanding soil microbial community like the effect:

  • Pollution on the community;
  • Fertilizers;
  • Plant;
  • Another treatments and soil managements.

CONCLUSIONS CONCLUSIONS

  • Another treatments and soil managements.

After reviewing all the studies we can affirm that MicroResp is an appropriate method, rapid and sensitive for determination of microbial community functional diversity.

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