International Genetically Engineered Machine 2013 Competition - - PowerPoint PPT Presentation

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Jan 20, 2023 5 likes •210 views

ITB_Indonesia International Genetically Engineered Machine 2013 Competition Indonesia and risk of aflatoxin Bad post harvesting and storage system can increase the growth of fungi such as Aspergillus sp % Commodity Contaminated by

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ITB_Indonesia International Genetically Engineered Machine 2013 Competition

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Indonesia and risk of aflatoxin

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Bad post harvesting and storage system can increase the growth of fungi such as Aspergillus sp

Farombi, et. al. (2006) Aflatoxin contamination of foods in developing ountries: Implications for hepatocellular carcinoma and chemopreventive strategies

8% 48% 8% 6% 5% 11% 3% 11%

% Commodity Contaminated by aflatoxin

Egypt Others India Malaysia Nepal Nigeria Turkey Indonesia

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Increasing susceptibility

f liver cancer*

*International Agency for Research of Cancer (IARC).

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Build a whole cell biosensor for aflatoxin B1

detection in foods

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In monitoring environment status (Polutans, toxic compounds, etc) we need : Accuracy Rapidity Conventional Sensor VS Biosensor

Biosensor
Biomolecule
Whole cell

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aflatoxin GFP

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CYP 3A4

Illegal Restriction Site

Remove the illegal restriction site

Signal peptide

Remove the signal peptide

Bba_K1064004

B0030

Add a strong RBS

Double terminator Bba_J23119

Bba_K1064000

Add a constitutive promotor

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SDS PAGE analysis of CYP expression

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Reporter Gene

Double terminator Promotor sensitive To DNA damage pUV

pUV + GFP (Bba_K1064003)

pSOS

pSOS + GFP (Bba_K1064002)

Part submitted Part submitted

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pUV pSOS

Control (No UV) UV irradiation (10s) Bright Field Microscope Fluorescence Microscope Bright Field Microscope Fluorescence Microscope

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44 46 48 50 52 54 56 58 60 62 Bba_K1064002 (pSOS+GFP) Bba_K1064003 (pUV+GFP) Fold GFP

pUV pSOS

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Goal To find time for analyzing aflatoxin presence in food with sensitivity threshold about 20 ppb*

*) Based on Indonesia Food and Drug Regulation

Aflatoxin diffusion CYP synthesis Enzymatic reaction DNA damage and SOS response Reporter

Simulate all phenomena!

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using Simbiology (computational biology toolbox from Mathworks Matlab) :

20 ppb 15 ppb 10 ppb 0 ppb 5 ppb 25 ppb 30 ppb 35 ppb 40 ppb 45 ppb

GFP (mole) (s)

minimum observable [GFP] minimum time needed for analysis

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Submitting part Bba_K1064002 and Bba_K1064003 Our construct works successfully as expected Characterize part Bba_K1064002 and Bba_K1064003 Improvement on part BBa_J22106 and Bba_I765001

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 Making prototype of our device  Adding another more visible chromopohore as reporter  Constructing the reactive sequence  amplifying the DNA damage signal  Adding buoyancy system  Make the device safer for user

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 Indonesian Potential to Synthetic Biology

synbio.itb.ac.id facebook.com/synbio.itb @synbio_itb

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 Project

Survey : Indonesian people awareness about aflatoxin and

the urgency of detector of it

32%

correspondent need a biosensor

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ARI Biotechnology INDRA Microbiology NUKE Microbiology RIANDY Informatic Engineering LILI Bioengineering DIMAS Chemical Engineering

Dr. Sony
Dr. Maelita
Dr. Adi

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Special Thanks To :

School of Life Sciences

and Technology

School of Electrical and

Informatics engineering

Faculty of Industrial

Technology