In vivo stepwise immunomodulation using chitosan nanoparticles as a - - PowerPoint PPT Presentation

In vivo stepwise immunomodulation using chitosan nanoparticles as a platform nanotechnology for cancer immunotherapy

The 15th Korea-U.S. Forum on Nanotechnology

Hee Dong Han

School of Medicine, Konkuk University

DC-based cancer immunotherapy

Injection of DCs Tumor microenvironment CD 8+ T cell activation Inhibition of tumor cells growth Mature DC Immature DC Intracellular delivery of antigen and adjuvant co-encapsulated PLGA-NPs PLGA-NPs Tumor cell

Engineered Nanoparticle

Overall concept

Han et al., Scientic Reports, 2016, doi: 10.1038/srep38348

Lymph node

Physical properties of CH-NPs

Han et al., Scientic Reports, 2016, doi: 10.1038/srep38348

Intracellular delivery of CH-NPs to DCs

Han et al., Scientic Reports, 2016, doi: 10.1038/srep38348

Maturation and activation of DCs induced by CH-NPs

Han et al., Scientic Reports, 2016, doi: 10.1038/srep38348

In vivo stepwise CH-NP-based active immune response

Han et al., Scientic Reports, 2016, doi: 10.1038/srep38348

Therapeutic efficacy of CH-NP treatment in EG.7 tumor model

Han et al., Scientic Reports, 2016, doi: 10.1038/srep38348

Summary

• We demonstrated that a novel in vivo active cancer immunotherapy based on CH-NP

loaded with an adjuvant and antigen to increase in vivo maturation of DCs lead to potent antigen-specific CD8+ T cell immunity.

• Our CH-NP system could be expanded and developed to include additional

therapeutic and experimental approaches.