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Impact of ionising radiation on aquatic organisms Adelaide - - PowerPoint PPT Presentation
Impact of ionising radiation on aquatic organisms Adelaide - - PowerPoint PPT Presentation
Impact of ionising radiation on aquatic organisms Adelaide Lerebours Jim Smith Neil Fuller Alex Ford EU INTAS Cooling Pond Project 2002-04 Fish diversity around
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Fish ¡diversity ¡around ¡Chernobyl ¡
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Increasing contamination →
Invertebrate diversity
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NERC Radioactivity and the Environment Programme
Transfer – Exposure – Effects: Integrating the science to underpin radioactivity assessments for humans and wildlife
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Laboratory experiments
- 4 exposure conditions 32P:
ü Control ü 0.1, 1 and 10 mGy/day
- 27 to 29 embryos/treatment
- Biometric analyses: embryos/larvae
ü Development ü Hatching success ü Physiology
- Presence of abnomalies
- Genotoxicity tests
- Novel mRNA biomarkers
Experimental units
In vitro fertilized embryos
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200 400 600 800 1000 1200 1400 1600 Control D10 Embryo diameter (um) +/- SEM
Diameter (um)
0.E+00 2.E+05 4.E+05 6.E+05 8.E+05 1.E+06 1.E+06 1.E+06 2.E+06 2.E+06 Control D10 Embryo surface (um2) +/- SEM
Surface (um2)
200 400 600 800 1000 Control D10 Embryo eye distance (um) +/- SEM
Eye distance (um)
Laboratory experiments
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20 40 60 80 100 120 140 160 180 Control Exposed Heart beats (beats/min) +/- SEM
Embryos (6 dpf) heart beats
Laboratory experiments
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Field experiments
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Comet assay Blood Expression of genes Liver Gonads Micronucleu s Blood smear Histology Liver Gonads
Field experiments
Age
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Dry shipper LN2 Deware LN2 supply Dry shipper
BELARU S UKRAINE
Field experiments
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Assessing the effects of radiations:
- From the individual to the molecular mechanistic
levels
- Taking into account confounding factors
- Focusing on reproduction and development
- Also studying aquatic invertebrate development