Impact ¡of ¡ionising ¡radiation ¡on ¡ aquatic ¡organisms ¡ Adelaide ¡Lerebours ¡ Jim ¡Smith ¡ Neil ¡Fuller ¡ Alex ¡Ford ¡
EU INTAS Cooling Pond Project 2002-04
Fish ¡diversity ¡around ¡Chernobyl ¡
Increasing contamination → Invertebrate diversity
NERC Radioactivity and the Environment Programme Transfer – Exposure – Effects: Integrating the science to underpin radioactivity assessments for humans and wildlife
Experimental units Laboratory experiments In vitro fertilized embryos • 4 exposure conditions 32 P: ü Control ü 0.1, 1 and 10 mGy/day • 27 to 29 embryos/treatment • Biometric analyses: embryos/larvae ü Development ü Hatching success ü Physiology • Presence of abnomalies • Genotoxicity tests • Novel mRNA biomarkers
0 400 2.E+06 Control D10 Embryo surface (um2) +/- SEM Surface (um2) 0 200 600 1.E+06 800 1000 Control D10 Embryo eye distance (um) +/- SEM Eye distance (um) 2.E+06 1.E+06 200 Control 400 600 800 1000 1200 1400 1600 D10 1.E+06 Embryo diameter (um) +/- SEM Diameter (um) 0.E+00 2.E+05 4.E+05 6.E+05 8.E+05 Laboratory experiments
0 20 40 60 80 100 120 140 160 180 Control Exposed Heart beats (beats/min) +/- SEM Embryos (6 dpf) heart beats Laboratory experiments
Field experiments
Comet assay Blood Expression of genes Liver Gonads Micronucleu s Blood smear Histology Liver Gonads Field experiments Age
Dry shipper LN2 Deware LN2 supply Dry shipper BELARU S UKRAINE Field experiments
Assessing the effects of radiations: levels and reproduction. Summary • From the individual to the molecular mechanistic • Taking into account confounding factors • Focusing on reproduction and development • Also studying aquatic invertebrate development
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