H 2 O Systems Update Presentation Paulo Jacob Jennifer Liang - - PowerPoint PPT Presentation

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H 2 O Systems Update Presentation Paulo Jacob Jennifer Liang - - PowerPoint PPT Presentation

Aug 13, 2022 334 likes •576 views

H 2 O Systems Update Presentation Paulo Jacob Jennifer Liang Jonathan Tejada Ami Yamamoto Joy Yuan March 16, 2006 Update Presentation Work Space, Storage, Advisors Changes in our design System construction Flow

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SLIDE 1

H2O Systems

Update Presentation

Paulo Jacob Jennifer Liang Jonathan Tejada Ami Yamamoto Joy Yuan March 16, 2006

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SLIDE 2

Update Presentation

  • Work Space, Storage, Advisors
  • Changes in our design
  • System construction
  • Flow visualization
  • Water dielectric breakdown problem
  • Cell lysis efficiency testing protocol
  • Works in progress
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SLIDE 3

Work Space, Storage, Advisors

  • Lab Space:

– Construction of system: 13-5032 – Anodizing: Professor Sadoway’s lab – Bacteria culture: 68-074 – Running water sample: 68-074

  • Refrigerator for bacterial culture:

– Undergraduate lab: Building 8

  • Outside Contacts:

– Michelle Mischke: Bacteria testing and culture – Professor Sadoway: Anodizing

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SLIDE 4

V = E · d d = V / E = (25V) / (1–5 × 105 V/m) = 50 – 250 µm Size of eukaryotic cell ~ 100 µm

Use 127µm electrode gap

Revising the Dimensions

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SLIDE 5

Steel Plate Dimensions

0.4 cm 5 cm 10 cm 1 cm 1.2 cm 1.9 cm 1.3 cm 1.9 cm

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SLIDE 6

Attachment Issues

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SLIDE 7

500 µm

Flow Visualization

Purpose: To confirm that water will flow through our system at a reasonable rate.

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SLIDE 8

Stainless Steel: Schematic

Tubing Syringe Shimstock Clamps Plastic plates Water in Water out

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SLIDE 9
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SLIDE 10

Result

Main Issue: Leakage from inlet/outlet Solution: Use sealant to minimize leakage

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SLIDE 11

500 µm

Modeling Water Flow

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SLIDE 12

127 µm

Titanium: Schematic

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SLIDE 13

Titanium

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SLIDE 14

Result

Conclusion:

  • Leakage from inlet/outlet
  • Pressure required for flow is very low
  • Water flows through the entire volume within

system

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SLIDE 15

Water Dielectric Breakdown

  • Concern was raised regarding water

dielectric breakdown and possible dangers associated with it.

  • Solution: IT WON’T BE A PROBLEM

– Water Dielectric Breakdown = 200~300kV/cm – Breakdown Potential for Cell Lysis: 1~5kV/cm – We are aiming at 5kV/cm<<200kV/cm

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SLIDE 16

Protocol for Bacteria Testing

  • Bacteria:

– E.Coli stock culture – Bacteria concentration: 5cfu/mL

  • Highest count in Charles River: ~5cfu/mL
  • Water Sample Preparation:

– 5mL culture + 35mL distilled water – Stock bacteria concentration: 40cfu/mL

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SLIDE 17

Experiment

  • Bacteria concentration quantification before

and after each run

  • Spectrophotometer measurement

– Blank: 5mL LB broth + 35mL distilled water – Using Beer’s Law, we will find bacteria concentration [ A = εcl ] – Decrease in concentration = Indication of cell lysis.

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SLIDE 18

Experiment Set Up

Water In Water Out

Power Supply

Weight

To control flow rate, will apply a constant load → constant pressure

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SLIDE 19

Work in Progress

  • Anodizing Titanium

– Consulting Professor Sadoway

  • Polarizing Stainless Steel and Ti

– Observe electrode reaction with H2O (corrosion).

  • Growing bacteria culture
  • Testing
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SLIDE 20

Gantt Chart

Modification Testing Final Presentation Preparation Construction Material Acquisition Design Research 5/11-5/18 4/27-5/10 4/6-4/26 3/16-4/5 2/23-3/15 2/9-2/22

Before spring break: Anodize Ti and polarize electrodes.

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SLIDE 21

Website

  • http://web.mit.edu/3.042/team3_06/update

s.html

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SLIDE 22

Question

  • Questions?