GSLE Introduction A New System for DNA Sequencing Orders March 24 th - - PowerPoint PPT Presentation
Northwestern University Feinberg School of Medicine GSLE Introduction A New System for DNA Sequencing Orders March 24 th , 2014 Welcome to GLSE Gene Sifter Lab Edition GSLE will be Replacing Corefac as our Ordering System for Traditional
Northwestern University Feinberg School of Medicine
A New System for DNA Sequencing Orders
March 24th, 2014
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Gene Sifter Lab Edition
as our Ordering System for Traditional Sequencing.
Welcome to GLSE
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Account Set-Up
To set up your GSLE and NuCore accounts email this information to: Sequencing@CGM .Northwestern.edu
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Log in to GSLE
NU Username is your Net ID. Non-NU Username is your email address. Password is not linked to your Net ID password. If you are logging in for the first time, enter your Username and click “Forgot Password?” to set your password.
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Select Service - 1
Click “Place Order”
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Select Service - 2
Click Button to
Begin Order
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Select Service - 3
Available Services More Information about each Service Click on the link for your service.
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Primer Information - 1
2) Click “Yes” if you are using primers that are not in our system. 1) Click “No” if you are using core supplied primers or primers you have already entered in GSLE.
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Primer Information - 2
1) Enter Primer Name 2) Leave “User Supplied” as source 3) Change Type to “User Supplied Primer” 5) Enter Concentration (1 uM), Volume and Description 6) Click “Save Primers” to Proceed. 4) Enter Primer Sequence
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Order Information
1) Customer NU Users = Your Net ID Non-NU Users = Your Email address 3) Order Name is for your reference only. 4) Select Template Type and Clean-Up Method from Menus. 5) Order comments are
2) Lab Group = Chart String or Account
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Sample Information
Excel Buttons to Download
Information 2) Enter Template name using no special characters 3) Primer names must match what is in the system exactly. Click on to select primers from a list. 4) Enter exact concentration for each sample in ng/uL
1) Enter your Range and click “Go”
5) Enter total volume for each sample. 6) Enter total vector size (backbone + insert) 8) Click “Next” to submit your samples. 7) Check box to have samples nanodropped.
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Sample Information - Individual and Plate Premix
Order information is the same for all services – see slide 10 for details Only Sample name, Primer and Total Volume are required for premix submissions. Use excel buttons to download template file and upload sample information.
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1) If there are any errors or missing information on the form, the section will be highlighted in red. 2) Arrow over for the cause of the error
Errors
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1) Make Changes to the Order 2) Delete the Order The order cannot be submitted until purchasing information is verified.
Purchasing Information - 1
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Purchasing Information - 2
1) Select your PI. 2) Select Your Chart String. 3) Click Next.
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Purchasing information - 3
Click Submit Order
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Order Complete
When you reach this page, your order has been submitted. You will receive a conformation email. If you do not receive a conformation, your order was not submitted.
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View and Download Results
We recommend downloading FinchTV to view your sequencing results. FinchTV is a free program for viewing sequencing files that integrates directly with GSLE. Click on the “Get FinchTV” Link in the upper right hand corner
The link will take you to the Perkins Elmer website where you can download FinchTV for free.
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View and Download Results
1) Click on the link in the email notification you received.
To View or Download a single sample, Click on the sample Label.
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View and Download Results
Text Sequence Quality Graph File Information
Open Chromatogram in Finch TV Open Chromatogram in Web Browser Download this page as a PDF Download Chromatogram File (instructions continue
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View and Download Results
Select the check box for .scf file; leave unchecked for .ab1. Click “Download Chromat” to download the file. Click “Nucleotides” for .fasta files Click “Quality Scores” for .qual files Click “Amino Acid Translations” for .txt translation Then Click “Download Sequence”. Click here to download .Phd File.
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View and Download Results
To download multiple files from a single order, click on the folder name.
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View and Download Results
Check the boxes for the files you want to download. Select “Download Chromat” from the drop down menu and click “Go”.
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View and Download Results
Select the check box for .scf file; leave unchecked for .ab1. Click “Download Chromat” Click “Nucleotides” for .fasta files Click “Quality Scores” for .qual files Click “Amino Acid Translations” for .txt translation Then Click “Download Sequence Click here to download .Phd File.
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Contact Us:
Phone: 312-503-2144 Email: Sequencing@CGM.Northwestern.edu Instructions:http://cgm.northwestern.edu/cores/genomics/sequencing/traditional- sequencing/index.html
Questions?
Web Site Address: nu-genomics.genesifter.net