Ge Genomic d c divi vision o of l labo bor du during co g - PowerPoint PPT Presentation

Ge Genomic d c divi vision o of l labo bor du during co g colony de y defense i in a a gent gentle le Afric fricanized anized honey honey bee. bee. Presented by: Arián Avalos

• Colony defense is a complex behavior derived from individual actions. • In honey bees, different groups of individuals participate, arriving at a concerted aggressive response. • Differences in genetics within colonies has been linked to colony defense (Page and Robinson, 1988) .

• Africanized bees in Puerto Rico recently, rapidly evolved, becoming gentler (gAHB) within 20 generations (Rivera-Marchand et al., 2012) . • The change was mediated by a soft selective sweep (Avalos & Pan et al., 2017)

Naïve Foragers n = 10 Stinging Collected at leather patches in • Soldiers front of the colony over a period of n = 10 2 min post-disturbance Pursuers Collected around researchers • n = 13 n = 10 after stepping ~3 m from the colony Number of Stings Post-Test Foragers Collected returning to the • n = 10 colony ~30 min post- Behavioral disturbance. Ranking Assay

Naïve Foragers n = 10 Stinging Soldiers n = 10 • A total of 200 samples were processed and sequenced, 100 from each of the target groups. Pursuers n = 10 Post-Test Foragers n = 10 Photos by: Alex Wild

Number of Stings Behavioral Ranking Assay Photos by: Alex Wild

Photos by: Alex Wild

-Log10 (p-value) Photos by: Alex Wild

Photos by: Alex Wild

Photos by: Alex Wild

• We identified 257 genes in our analysis, 56 of which also had strong signal of positive selection. • Genes on this list were significantly enriched for terms associated with immunoglobulin domain. • Our top candidate was the orthologue of Drosophila defective proboscis response 4 ( dpr4 ) gene.

Geno Genotype pe (AA, AB, BB) Be Behavior Photos by: Alex Wild

Ackn knowledgments • Co Collaborators and Co Contributors • Fund Funding ng So Sour urces es: • Miaoquan Fang • Hailin Pan • Amy Ahmed-Cash • Aixa Ramirez Lluch • Fanfan Noel • Gloria Rendon • Chris Fields • Tugrul Giray • Gene E. Robinson • Matthew E. Hudson • Guojie Zhang

Questions? Photo: http://ahealthylifeforme.com/how-to-set-up-a-beehive/