Feasibility of Fungi to Remove Heavy Metals from Mine Wastewater - - PowerPoint PPT Presentation

Feasibility of Fungi to Remove Heavy Metals from Mine Wastewater

Prepared by: Masad Alyahya, Nolan Maxwell, Yue Shen Date: February 25, 2020

CENE 476-Proposal

Introduction

• Purpose: Test the ability of fungi to remove heavy metals from

mine wastewater.

• Client: Dr. Bridget Bero
• Location: NAU Environmental (ENE) Lab & Science Lab Facility

(SLF) Mycology Lab

• Background:Previous research showed that Aspergillus sydowii

is capable of removing Chromium and Fusarium verticillioides is capable of removing Calcium and Magnesium from water [1][2].

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Project Scope

Task 1: Select Fungi and Metals Contaminants

Task 1.1 Literature Review:

• Determine 2 types of fungi and metals

Task 1.2 Interview A Mycologist

• Get some informations and suggestions

Task 1.3 Final Selection of Fungi and Metals

• Decision Matrix

3 Figure 1: Cultivated Sample of Aspergillus sydowii [3]

Project Scope

Task 2: Cultivate Fungi

Task 2.1 Authorize Environmental Engineering Laboratory Use

• Create a Protocol Binder

Task 2.2 Obtain and Growth Fungi Specimens

• Purchase Spawn
• Growth Medium (2 - 4 weeks)

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Task 2.2.1 Sterilization

• Autoclave

Task 2.2.2 Inoculation and Growth

• Laminar Flow Hood
• Storage within Lab

Project Scope

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Task 3: Experimental Design and Preparation

Task 3.1 Design Experimental Matrix

• Replicants (the number of trials)
• Control variables (Acidity, uptake capacity, temperature)
• Control samples

Task 3.2 Preparation of Experiment

• Supplies

Project Scope

Task 4: Perform Experiment

• Introducing fungi and control

samples to contaminated water.

• Using a shaker to keep the

samples well-mixed.

• Obtaining data for a single fungi

species and metal contaminant.

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Task 5: Data Analysis

Task 5.1: WW Analysis in WW Samples

• XRF, FAA, or ICP

Task 5.2: Fungi Analysis

• Sorbed metal conc. analysis.

Task 5.3: Create Adsorption Isotherms

• Relationship of sorbate to sorbent

Project Scope

Task 6: Project Deliverables

Task 6.1-6.4: 30%, 60%, 90%, and Final Report and Presentation

• Create project proposal and

presentation

• Improve from feedback on previous

submittals

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Task 6.5: Website

• Create final website to allow

information to be easily accessible to the public

• Proposal, presentation, schedule

Project Scope

Task 7: Evaluate Impacts

Task 7.1: Regulations

• Wastewater MCLs, MCLGs

Task 7.2: Environment, Social, and Economic Impacts

• Reduce toxicity/damage
• Protect waterways
• Possibly more cost effective

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Task 8: Project Management

Task 8.1: Resource Management

• Time, funds, materials, toxics

Task 8.2: Meetings

• Client, TA, GI, team members

Task 8.3: Schedule

• Timeline for task completion

Project Scope

Exclusions

• Field testing

9 Figure 2: Cultivated Samples of Fusarium verticillioides [4] Figure 3: Gold king mine spill at Silverton Colorado [5]

References

[1] "Fungi and Lichens of Great Britain and Ireland: Aspergillus sydowii," [Online]. Available: http://fungi.myspecies.info/taxonomy/term/4992/media. [Accessed 17 February 2020]. [2] X. Gai, H. Dong, S. Wang, B. Liu, Z. Zhang, X. Li and Z. Gau, "Infection cycle of maize stalk rot and ear rot caused by Fusarium verticillioides," PLOS ONE, vol. 13, no. 7, 2018. [3]"Fungi and Lichens of Great Britain and Ireland: Aspergillus sydowii," [Online]. Available: http://fungi.myspecies.info/taxonomy/term/4992/media. [Accessed 17 February 2020].

[4] X. Gai, H. Dong, S. Wang, B. Liu, Z. Zhang, X. Li and Z. Gau, "Infection cycle of maize stalk rot and ear rot caused by Fusarium verticillioides," PLOS ONE, vol. 13, no. 7, 2018. [5] "Aug 18, 2016 - Navajo Nation Sues EPA over Gold King Mine Spill," Last Real Indians, 18 August 2016. [Online]. Available: https://lastrealindians.com/news/2016/8/18/aug-18-2016-navajo-nation-sues-epa-over-gold-king-mine-spill. [Accessed 24 February 2020].

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Questions?

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