Down-Regulation of miRNAs by Mutated FOXP2 Ellen Korcovelos May - - PowerPoint PPT Presentation
Down-Regulation of miRNAs by Mutated FOXP2 Ellen Korcovelos May 11, 2016 BNFO 300 Issue at hand World Dementia Prevalence Dementia Number of People (millions) 81 62 Neurodegeneration Memory Loss Speech Deficiencies 42 33
Ellen Korcovelos May 11, 2016 BNFO 300
24 33 42 62 81 2000 2010 2020 2030 2040 Number of People (millions) Year
World Dementia Prevalence
Dementia
Neurodegeneration Memory Loss Speech Deficiencies
Alzheimer’s Disease Mild Cognitive Impairment Frontotemporal Dementia Primary Progressive Aphasia Vascular Dementia
RISC mRNA miRNA Mature miRNA
RISC mRNA miRNA Mature miRNA mRNA decay gene expression
RISC mRNA miRNA Mature miRNA BACE1 APP FOXP2
RISC mRNA miRNA Mature miRNA APP Aβ42 Aβ42 Aβ42
RISC mRNA miRNA Mature miRNA APP Aβ42 FOXP2 FOXP2
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Cell Preparation
Murine frontotemporal cells ½ unaltered FOXP2 ½ point mutation affected FOXP2
Extracting the miRNA
Buffer lyses tissue DNA removed by centrifugation RNA binding membrane
Analyzing Expression
TaqMan Low-Density Microarray Rt-PCR
Cell Preparation
Murine frontotemporal cells ½ unaltered FOXP2 ½ point mutation affected FOXP2
Extracting the miRNA
Buffer lyses tissue DNA removed by centrifugation RNA binding membrane
Analyzing Expression
TaqMan Low-Density Microarray Rt-PCR
Cell Preparation
Murine frontotemporal cells ½ unaltered FOXP2 ½ point mutation affected FOXP2
Extracting the miRNA
Buffer lyses tissue DNA removed by centrifugation RNA binding membrane
Analyzing Expression
TaqMan Low-Density Microarray Rt-PCR
Cell Preparation
Murine frontotemporal cells ½ unaltered FOXP2 ½ point mutation affected FOXP2
Extracting the miRNA
Buffer lyses tissue DNA removed by centrifugation RNA binding membrane
Analyzing Expression
TaqMan Low-Density Microarray Rt-PCR