Carroll County Career Carroll County Career Carroll County Career - - PowerPoint PPT Presentation

Carroll County Career Carroll County Career Carroll County Career Carroll County Career and Technology Center and Technology Center and Technology Center and Technology Center

• Dynamic and rigorous high school

Dynamic and rigorous high school Dynamic and rigorous high school Dynamic and rigorous high school curriculum that emphasizes: curriculum that emphasizes: curriculum that emphasizes: curriculum that emphasizes:

• Real world experience

Real world experience Real world experience Real world experience

• Hands on learning

Hands on learning Hands on learning Hands on learning

• Provides a doorway for students to

Provides a doorway for students to Provides a doorway for students to Provides a doorway for students to explore and become a part of a medical explore and become a part of a medical explore and become a part of a medical explore and become a part of a medical based field based field based field based field

• Coursework that expands upon college

Coursework that expands upon college Coursework that expands upon college Coursework that expands upon college-

• prep science and math by using activity,

prep science and math by using activity, prep science and math by using activity, prep science and math by using activity, project, and problem project, and problem project, and problem project, and problem-

• based learning to

based learning to based learning to based learning to create an exciting learning environment create an exciting learning environment create an exciting learning environment create an exciting learning environment

• Students will learn valuable, real

Students will learn valuable, real Students will learn valuable, real Students will learn valuable, real-

• world skills such as:

world skills such as: world skills such as: world skills such as:

• Problem solving

Problem solving Problem solving Problem solving

• Working as a contributing team member

Working as a contributing team member Working as a contributing team member Working as a contributing team member and leading a team and leading a team and leading a team and leading a team

• Conducting research

Conducting research Conducting research Conducting research

• Data analysis

Data analysis Data analysis Data analysis

• Managing time, resources, and projects

Managing time, resources, and projects Managing time, resources, and projects Managing time, resources, and projects

• Written and oral communication

Written and oral communication Written and oral communication Written and oral communication

• Going beyond the classroom to learn and

Going beyond the classroom to learn and Going beyond the classroom to learn and Going beyond the classroom to learn and find answers find answers find answers find answers

• All students interested in pursuing a

All students interested in pursuing a All students interested in pursuing a All students interested in pursuing a career in biological sciences, career in biological sciences, career in biological sciences, career in biological sciences, biotechnology, or medical research biotechnology, or medical research biotechnology, or medical research biotechnology, or medical research

• All students prepared for a rigorous

All students prepared for a rigorous All students prepared for a rigorous All students prepared for a rigorous and relevant curriculum that and relevant curriculum that and relevant curriculum that and relevant curriculum that provides hands provides hands provides hands provides hands-

• on learning with
• n learning with
• n learning with
• n learning with

real real real real-

• world scientific research

world scientific research world scientific research world scientific research techniques techniques techniques techniques

• Principles of

Principles of Principles of Principles of Biomedical Sciences Biomedical Sciences Biomedical Sciences Biomedical Sciences

• Human Body

Human Body Human Body Human Body Systems Systems Systems Systems

• Medical

Medical Medical Medical Interventions Interventions Interventions Interventions

• Biomedical

Biomedical Biomedical Biomedical Innovations Innovations Innovations Innovations

• Medical Professionals

Medical Professionals Medical Professionals Medical Professionals

• Pathol

Pathologists

• gists

Pathol Pathologists

• gists
• Specialist

Specialists Specialist Specialists

• Medical Researchers

Medical Researchers Medical Researchers Medical Researchers

• Researchers

Researchers Researchers Researchers

• Private /Government

Private /Government Private /Government Private /Government /University /University /University /University

• Laboratory

Laboratory Laboratory Laboratory technicians technicians technicians technicians

• Forensics

Forensics Forensics Forensics

• CS

CSI / DNA expert / I / DNA expert / CS CSI / DNA expert / I / DNA expert / Anthropologist Anthropologist Anthropologist Anthropologist

• Epidemiologist

Epidemiologist Epidemiologist Epidemiologist

• Geneticist

Geneticist Geneticist Geneticist

• Biochemist

Biochemist Biochemist Biochemist

• Microbiologist

Microbiologist Microbiologist Microbiologist

• Neurologist

Neurologist Neurologist Neurologist

• Sports Medicine

Sports Medicine Sports Medicine Sports Medicine

• Dietician

Dietician Dietician Dietician

• Immunologist

Immunologist Immunologist Immunologist

Cara Jones Cara Jones Cara Jones Cara Jones Hannah Langsdale Hannah Langsdale Hannah Langsdale Hannah Langsdale

• Use recombinant DNA technology to

Use recombinant DNA technology to Use recombinant DNA technology to Use recombinant DNA technology to insert pGLO gene insert pGLO gene insert pGLO gene insert pGLO gene

• pGLO

pGLO pGLO pGLO – – codes for protein that causes green fluorescence codes for protein that causes green fluorescence codes for protein that causes green fluorescence codes for protein that causes green fluorescence

• Insert the pGLO gene into a plasmid

Insert the pGLO gene into a plasmid Insert the pGLO gene into a plasmid Insert the pGLO gene into a plasmid

• “

“Cut and Cut and paste paste Cut and Cut and paste paste” ” using restriction enzymes using restriction enzymes using restriction enzymes using restriction enzymes

• Insert plasmid into

Insert plasmid into Insert plasmid into Insert plasmid into E. coli

• E. coli
• E. coli
• E. coli bacteria cells

bacteria cells bacteria cells bacteria cells by by by by transformation

transformation transformation transformation

• If gene is expressed

If gene is expressed If gene is expressed If gene is expressed → → bacteria glow green bacteria glow green bacteria glow green bacteria glow green

+pGLO on LB / amp +pGLO on LB / amp +pGLO on LB / amp / ara +pGLO on LB / amp / ara

• Polyacrylamide Gel Electrophoresis

Polyacrylamide Gel Electrophoresis Polyacrylamide Gel Electrophoresis Polyacrylamide Gel Electrophoresis

• Proteins travel through gel according to

Proteins travel through gel according to Proteins travel through gel according to Proteins travel through gel according to size size size size

• Examine banding patterns to determine

Examine banding patterns to determine Examine banding patterns to determine Examine banding patterns to determine success of protein purification success of protein purification success of protein purification success of protein purification

Purified Purified GFP Protein GFP Protein

• Goal: clone and sequence glyceraldehyde

Goal: clone and sequence glyceraldehyde Goal: clone and sequence glyceraldehyde Goal: clone and sequence glyceraldehyde 3 3-

• phosphate dehydrogenase gene

phosphate dehydrogenase gene phosphate dehydrogenase gene phosphate dehydrogenase gene (GAPDH) in a plant species (GAPDH) in a plant species (GAPDH) in a plant species (GAPDH) in a plant species

• Published if we obtain a unique sequence

Published if we obtain a unique sequence Published if we obtain a unique sequence Published if we obtain a unique sequence

• Extracted and purified plant DNA

Extracted and purified plant DNA Extracted and purified plant DNA Extracted and purified plant DNA

• PCR

PCR PCR PCR

• Technique to

Technique to amplify amplify Technique to Technique to amplify amplify GAPC GAPC GAPC GAPC gene from gDNA gene from gDNA gene from gDNA gene from gDNA

• Produce more copies of gene

Produce more copies of gene Produce more copies of gene Produce more copies of gene

• Purify PCR products by small exclusion

Purify PCR products by small exclusion Purify PCR products by small exclusion Purify PCR products by small exclusion chromatography chromatography chromatography chromatography

• Inserted PCR product (

Inserted PCR product ( Inserted PCR product ( Inserted PCR product (GAPC GAPC GAPC GAPC gene) into gene) into gene) into gene) into pJet1.2 blunted plasmid vector pJet1.2 blunted plasmid vector pJet1.2 blunted plasmid vector pJet1.2 blunted plasmid vector

• DNA ligase

DNA ligase DNA ligase DNA ligase -

• joined two pieces of DNA into

joined two pieces of DNA into joined two pieces of DNA into joined two pieces of DNA into a single piece a single piece a single piece a single piece

• Vector inserted into

Vector inserted into Vector inserted into Vector inserted into

• E. coli
• E. coli
• E. coli
• E. coli via

via via via transformation transformation transformation transformation

• Bacterial growth

Bacterial growth Bacterial growth Bacterial growth produces multiple, produces multiple, produces multiple, produces multiple, exact copies of G exact copies of G exact copies of G exact copies of GAPC APC APC APC gene (cloning) gene (cloning) gene (cloning) gene (cloning)

• Plasmid purification

Plasmid purification Plasmid purification Plasmid purification

• Lyse bacterial cells and isolate plasmid from

Lyse bacterial cells and isolate plasmid from Lyse bacterial cells and isolate plasmid from Lyse bacterial cells and isolate plasmid from culture culture culture culture

• Sent to laboratory facility where DNA is

Sent to laboratory facility where DNA is Sent to laboratory facility where DNA is Sent to laboratory facility where DNA is sequenced using an automatic sequenced using an automatic sequenced using an automatic sequenced using an automatic sequencer sequencer sequencer sequencer

• Determine the exact order of nucleotide bases

Determine the exact order of nucleotide bases Determine the exact order of nucleotide bases Determine the exact order of nucleotide bases

• Rigorous/relevant career program

Rigorous/relevant career program Rigorous/relevant career program Rigorous/relevant career program

• Program inception 2009

Program inception 2009 Program inception 2009 Program inception 2009

• First completers 2010 graduates

First completers 2010 graduates First completers 2010 graduates First completers 2010 graduates— —11dual 11dual 11dual 11dual completers completers completers completers

• Anticipated 2011graduates

Anticipated 2011graduates Anticipated 2011graduates Anticipated 2011graduates— —16 dual completers 16 dual completers 16 dual completers 16 dual completers

• Enrollment for anticipated 2012 graduates

Enrollment for anticipated 2012 graduates Enrollment for anticipated 2012 graduates Enrollment for anticipated 2012 graduates— —37 37 37 37

• 90%

90% of students are enrolled in AP courses

• f students are enrolled in AP courses

90% 90% of students are enrolled in AP courses

• f students are enrolled in AP courses
• Certification

Certification Certification Certification

• PLTW site certification visit

PLTW site certification visit PLTW site certification visit PLTW site certification visit— —October 27, 2010 October 27, 2010 October 27, 2010 October 27, 2010

• First fully certified PLTW Biomedical site in

First fully certified PLTW Biomedical site in First fully certified PLTW Biomedical site in First fully certified PLTW Biomedical site in Maryland Maryland Maryland Maryland

• Students eligible for 4 transcripted credits for

Students eligible for 4 transcripted credits for Students eligible for 4 transcripted credits for Students eligible for 4 transcripted credits for Biology 113 from Stevenson University Biology 113 from Stevenson University Biology 113 from Stevenson University Biology 113 from Stevenson University

• Continue to expand and diversify the

Continue to expand and diversify the Continue to expand and diversify the Continue to expand and diversify the Program Advisory Committee (PAC) Program Advisory Committee (PAC) Program Advisory Committee (PAC) Program Advisory Committee (PAC)

• Questions ?

Questions ? Questions ? Questions ?