Calcium Activation and Profiles in Dendritic Spines BENG260 6 Dec - - PowerPoint PPT Presentation

Calcium Activation and Profiles in Dendritic Spines

BENG260 6 Dec 2017 Miriam Bell and Michael Getz

Dendritic spines

• associated with memory and learning
• physical location of neural connections
• changes in spine size and shape

Phillips, Mary, and Lucas Pozzo-Miller. "Dendritic spine dysgenesis in autism related disorders." Neuroscience letters 601 (2015): 30-40. https://en.wikipedia.org/wiki/Dendritic_spine#/ media/File:Dendritic_spines.jpg

ER Postsynaptic area Endosomes/transport vesicles

The complex organization of the dendritic spine

de Camilli et al, PNAS 2017

AMPAR Ca2+-permeable AMPAR Ca2+ Voltage gated channels NMDAR MSIC

Ca2+

Nucleus ER Mitochondria Pumps

Plasma Membrane ER Membrane

!"#$% !&

= NMDAR + Ca Channels + ER stores – PM pump – ER pump - Buffers

NMDAR PM Channels - VSCC PM Pumps - PMCA, NCX ER Channels - ER membrane PM Pumps - SERCA RyR - CICR Calcium dynamics

Ordinary Differential Equations

• Cytosolic Calcium
• ER Calcium
• w dynamics

Plasma membrane ER membrane ER membrane

Membrane Voltage

• Prescribed input
• set of exponentials
• Vrest
• Back propagating action potentials
• Excitatory post-synaptic potentials
• Spike time dependent plasticity

!

" = ! %&'( + *+,+ + -+.+

Source: Shouval 2002, Jahr 1993, Mellor 2010

NMDAR channel activation

!" = $[&'()*+,-(../.,+

01

]

Source: Jahr 1993

Results

ms ms ms ms ms ms

Extra Slides

Source: Bartol et al 2015

VSCC - Ca2+ Channels PM

)

Source: Calizo in review

PM Pumps

ER Pumps - SERCA

Source: Eungdamrong 2007

Ca2+ Channels ER

Source: Calizo In review ["#$%

&'] = 200,-

ER Stores

Ryanodine Receptors (RyR) - Ca2+ gated Ca2+ channels Source: Friel 1995 !"#" = %&'( )*+"

', − )*.#/0 ',

) 34.567