Calcium Activation and Profiles in Dendritic Spines BENG260 6 Dec - PowerPoint PPT Presentation

Calcium Activation and Profiles in Dendritic Spines BENG260 6 Dec 2017 Miriam Bell and Michael Getz

Dendritic spines • associated with memory and learning • physical location of neural connections • changes in spine size and shape https://en.wikipedia.org/wiki/Dendritic_spine#/ media/File:Dendritic_spines.jpg Phillips, Mary, and Lucas Pozzo-Miller. "Dendritic spine dysgenesis in autism related disorders." Neuroscience letters 601 (2015): 30-40.

The complex organization of the dendritic spine ER Postsynaptic area Endosomes/transport vesicles de Camilli et al, PNAS 2017

Ca 2+ -permeable AMPAR Ca 2+ Voltage AMPAR gated channels Ca 2+ NMDAR ER Pumps MSIC Nucleus Mitochondria

Calcium dynamics !"# $% = NMDAR + Ca Channels + ER stores – PM pump – ER pump - Buffers !& Plasma Membrane NMDAR PM Channels - VSCC PM Pumps - PMCA, NCX ER Membrane ER Channels - ER membrane PM Pumps - SERCA RyR - CICR

Ordinary Differential Equations • Cytosolic Calcium • ER Calcium • w dynamics Plasma membrane ER membrane ER membrane

Membrane Voltage • Prescribed input • set of exponentials • Vrest • Back propagating action potentials • Excitatory post-synaptic potentials • Spike time dependent plasticity ! " = ! %&'( + *+,+ + -+.+ Source: Shouval 2002, Jahr 1993, Mellor 2010

NMDAR channel activation Source: Jahr 1993 01 ! " = $[&' ()*+,-(../.,+ ]

Results

ms ms ms ms ms ms

Extra Slides

VSCC - Ca 2+ Channels PM ) Source: Bartol et al 2015

PM Pumps Source: Calizo in review

ER Pumps - SERCA Source: Eungdamrong 2007

Ca 2+ Channels ER ["# $% &' ] = 200,- Source: Calizo In review

ER Stores Ryanodine Receptors (RyR) - Ca 2+ gated Ca 2+ channels ', − )* .#/0 ', ! "#" = % &' ( )* +" ) 3 4.567 Source: Friel 1995