Bio Detectors Accurate and precise Stable system Fast and visible - - PDF document

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Bio Detectors Accurate and precise Stable system Fast and visible - - PDF document

Nov 06, 2023 283 likes •692 views

Introduction Bio Detectors Accurate and precise Stable system Fast and visible response Versatile Our Idea Our Idea Fast Visible Output Accurate Detection

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SLIDE 1
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SLIDE 2

Bio Detectors

Introduction

  • Accurate and precise

Fast and visible response Stable system Versatile

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SLIDE 3

Our Idea

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SLIDE 4

Fast Visible Output Accurate Detection Bio-Detector

Our Idea

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SLIDE 5

Genetic Design

Genetic Design

Fast Visible Output Accurate Detection Bio-Detector

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SLIDE 6

Input Output

  • Genetic Design
  • Fast Visible Output

Accurate Detection Bio-Detector

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SLIDE 7

Dose Response

Genetic Design

  • RBS

LuxR Plac

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SLIDE 8

A Bi-Stable System

Genetic Design

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SLIDE 9

A Bi-Stable System

Signal Analyte Concentration

On Off

Bistable

Genetic Design

  • Dose response
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SLIDE 10

Design Modeling Assembly Experiments

Improving work methods

Genetic Design

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SLIDE 11

RBS LuxR Plac RBS POmpC LuxI

EcoRI NheI

RBS RBS

Reporter Protein

LuxI Plux

OmpR OmpR LuxI

AHL

LuxR

AHL

P

AHL AHL AHL AHL AHL AHL AHL AHL

IPTG

Design

Modeling

Assembly

Experiments

Genetic Design

  • 1
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SLIDE 12

Basic Design Modeling

Design

Modeling

Assembly

Experiments

Genetic Design

  • 1

Starts ON Starts OFF

Fokker-Planck solutions- Stochastic approach:

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SLIDE 13

Design Modeling

Assembly

Experiments

High-throughput Cloning

  • 3

4

RBS

2

Gene 1

Suffix

5

Prefix

6 1

Prefix

6 1 4 3

RBS Suffix

5 2 3 4

RBS

2

Gate Class 1 Gene 2

Genetic Design

  • 1
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SLIDE 14

Design Modeling

Assembly

Experiments Genetic Design

  • 1
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SLIDE 15

Design

Modeling

Assembly

Experiments

Genetic Design

  • 2

RNA Splint – Noise Controller

Plux Plux

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SLIDE 16

RNA Splint Modeling

Design

Modeling

Assembly

Experiments

Genetic Design

  • 2

Alternative-RNA Splint Basic Switch

Fokker-Planck solutions Starts at ON state: Fokker-Planck solutions Starts at OFF state:

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SLIDE 17

Design Modeling

Assembly

Experiments

High-throughput Cloning

  • 3

4

RBS

2

Gene 1

Suffix

5

Prefix

6 1

Prefix

6 1 4 3

RBS Suffix

5 2 3 4

RBS

2

Gate Class 1 Gene 2

Genetic Design

  • 2
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SLIDE 18

Fast Visible Output Accurate Detection Bio-Detector

Photo Switch

Photo Switch

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SLIDE 19

Introduction Synthetic Biofilm High- throughput Summary

Photo Switch

  • AHL

AHL AHL

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SLIDE 20

Photo Switch

  • AHL

Introduction Synthetic Biofilm High- throughput Summary

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SLIDE 21

Communicative & reversible Synthetic Biofilm

Photo Switch

  • The Photo Switch

Introduction Synthetic Biofilm High- throughput Summary

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SLIDE 22

With Azobenzene

Photo Switch

  • Without Azobenzene

Under the Microscope

Introduction Synthetic Biofilm High- throughput Summary

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SLIDE 23

The transition to the “sticky” form

  • ccurs with UV light

Azobenzene – The magical molecule

Photo Switch

  • Introduction

Synthetic Biofilm High- throughput Summary

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SLIDE 24

High-throughput Experiments

  • Photo Switch
  • Introduction

Synthetic Biofilm High- throughput Summary

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SLIDE 25

Bacterial Sediments

Sand falling creates a round shape out of symmetry Without Azobenzene

Photo Switch

  • Introduction

Synthetic Biofilm High- throughput Summary

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SLIDE 26

With Azobenzene With Azobenzene Without Azobenzene

Photo Switch

  • Bacterial Sediments

Introduction Synthetic Biofilm High- throughput Summary

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SLIDE 27

Photo Switch

  • Introduction

Synthetic Biofilm High- throughput Summary

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SLIDE 28

Set out to build a universal bio-detector for low- concentrations Designed a new protocol for building bi-stable biological systems (4 bi-stable systems) New high-throughput cloning (23 BioBricks) Created a synthetic bio-film

A smart self assembling material

So what did we do?

  • Achievements
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SLIDE 29

Photo Switch

  • Merge of Fields, Merge of Cultures
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SLIDE 30

Photo Switch

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SLIDE 31

Photo Switch

  • 1st iGEM High School Israel Team
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SLIDE 32

Special thanks to our mentors

  • Dr. Roee Amit
  • Dr. Orna Atar

Lior Levy Inbal Vaknin Michal Brunwasser Noa Katz

And to Our Sponsors

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SLIDE 33
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SLIDE 34

Genetic Design

  • RBS

LuxR Plac RBS POmpC LuxI

EcoRI NheI

RBS RBS Reporter Protein LuxI Plux

Design Modeling

Assembly

Experiments

Antenna Activator Amplifier

1

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SLIDE 35

Design

Modeling

Assembly

Experiments

RBS Plac CI RBS PCI LacI T7 polymerase

Genetic Design

  • 2

Toggle Switch

LacI CI

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SLIDE 36

Stable Design Modeling

Design

Modeling

Assembly

Experiments

Genetic Design

  • 2

With Analyte Without Analyte

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SLIDE 37

Achievements

  • Designed a new protocol for building

bistable biological systems Submitted 23 BioBricks New high-throughput cloning method Created synthetic biofilm

  • bling material

ughput cloning method

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SLIDE 38

Photo Switch

  • Introduction

Synthetic Biofilm High- throughput Summary

A smart self assembling material

The Photo Switch

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SLIDE 39

3 4 2

Gene 1

5

Prefix

6 1

Suffix