Asya Rolls, Damien Colas, Antoine Adamantidis, Matt Carter, Tope - - PDF document

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Optogenetic Manipulation of Sleep Continuity Impairs Memory Consolidation

Asya Rolls, Damien Colas, Antoine Adamantidis, Matt Carter, Tope Lanre-Amos, H Craig Heller, and Luis de Lecea

Presented by Brian Jacobs and Sam Doernberg BIONB 4110 Undergraduate Journal Club in Neuroscience

The Journal

• PNAS
• IF=9.861
• Very Popular Journal With relatively high impact

factor

• Dr. Craig Heller

PhD Yale University (1970) Professor of Biology and Physiology-Stanford University

• Dr. Asya Rolls
• PhD The Technion-Israel (2002)
• Professor at Stanford University
• PhD in psychiatry
• Biochemistry

Luis De-Lecea

• PhD in Molecular Biology University of

Barcelona (1991)

• Professor at Stanford Medical School
• Psychiatry and Behavior-Sleep Center

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• Antoine Adamantidis PhD(post doc)
• Damien Colas PhD (post doc)
• Matt Carter-PhD (post doc)
• Tope Lanre-Amos (Med Student)

The Rest of the Staff

Memory Creation in the Brain

What are the inherent problems with sleep studies?

• Duration
• Drugs
• Continuity
• Intensity
• Stress
• Flexibility

What is the theorized role of HCRT neurons in sleep?

Optogenetics

• Recombinant DNA
• Sensitivity to light
• Allows for control

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Intro

• Difficult to control
• Difference between NREM and REM
• Inherent problems with external stimuli
• Hypocretin/orexin neurons
• Channel Rhodopsin

Methods

• Optogenetic Stimulation
• Channel Rhodopsin stimulation
• NOR Test

Results

Results (contd.) Results (contd.)

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Results (Contd.) Results (contd.) Results (contd.) Discussion

• From an Evolutionary standpoint why

are the findings of this study intriguing/confusing?

• Continuity vs. memory performance
• Duration vs. memory performance
• What could this mean for certain

memory impairments and diseases?