aradulov@uoguelph.ca BOLD components and services ID Service DNA - - PowerPoint PPT Presentation

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Dec 31, 2022 219 likes •442 views

www.boldsystems.org Adriana Radulovici aradulov@uoguelph.ca BOLD components and services ID Service DNA Barcode Database Workbench BOLD component databases No account needed Data mining Data mining Application Programming Interface

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Adriana Radulovici aradulov@uoguelph.ca

www.boldsystems.org

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BOLD components and services

Workbench DNA Barcode Database ID Service

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BOLD component databases

No account needed

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Data mining

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Data mining

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Application Programming Interface

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BOLD database

Animals

192,000 species +365,000 BINs

Plants

67,200 species +8,500 Interim names

Fungi

17,700 species +11,000 Interim names

Protists

2,560 species +2,040 Interim names

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BOLD workbench

Username: Password:

PROJECT

Project manager Collaborators (view and/or edit contents)

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Specimen IDs Trace Data Geography DNA Sequence Audit Trail Taxonomy Images Primers Specimen details

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BOLD core data

Specimen Data Specimen Images Sequences Trace Files Custom Fields

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Data validation

Automated Validation Integrated Validation Independent Validation

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BOLD: storage, validation, analysis & publication

f DNA

barcode data

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Barcode Index Number (BIN)

BOLD:AAA3297

Sequence based OTU designation by clustering all

COI sequences in BOLD (once a month)

Automatic, standardized, persistent

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Species vs. BINs

A a A a B b A a1 a2 A a1 B a2 b

MATCH MERGE SPLIT MIXTURE

Species BINs

Lab issues: cross-contamination, plate flips
Taxonomic issues: misidentification, synonymy
Biology: cryptic species, young species, introgression,

hybridization

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A unique page for each BIN

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Data life cycle

Generate Upload Validate Analyze Publish

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Data life cycle

Re- visit

Generate Upload Validate Analyze Publish