april 21 2016 4mgen14 contamination sleuthing the problem
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April 21, 2016 4MGEN14 contamination Sleuthing the problem 4MGEN14 - PowerPoint PPT Presentation

ABC IAC April 21, 2016 4MGEN14 contamination Sleuthing the problem 4MGEN14 seed overwinter Lewisetta Rap Lynnhaven 5K 5K 5K split on site split on site split at VIMS 2n 14 10 2015 distribution 4MGEN14 seed overwinter Lewisetta


  1. ABC IAC April 21, 2016

  2. 4MGEN14 contamination Sleuthing the problem

  3. 4MGEN14 seed overwinter Lewisetta Rap Lynnhaven 5K 5K 5K split on site split on site split at VIMS 2n 14 10 2015 distribution

  4. 4MGEN14 seed overwinter Lewisetta Rap Lynnhaven 5K 5K 5K split on site split on site split at VIMS 2n 14 10 58%

  5. 2016 CERTIFICATIONS to date A B C D E F 4MGEN13 — 40/41 23/25 — — 20/20 98% 92% 100% 4MGEN14 30/32 — 14/15 43/43 21/23 5/7 80% 93% 100% 91% 71% All certs so far 2016 176/186 (95%) 2015 345/375 (92%) 2014 327/332 (99%) 2013 371/385 (96%) 2012 428/480 (89%)

  6. Brood stock quality Industry based research

  7. Glycogen and Fecundity • NIRS technology • Oyster Seed Holdings • LOLA, DEBY.13, DEBY.14 • Glycogen at time zero and just prior to conditioning – Different treatments prior to conditioning • Fecundity/egg counts on spawning day

  8. Growth

  9. Egg count vs. Glycogen

  10. Egg Count vs. Length

  11. False winter and Dermo • Belief that false winter aids in conditioning and “resets” Dermo if present • H° : oysters put through false winter will have lower prevalence and intensity of Dermo compared to those not put through false winter

  12. DEBY.LYN.13

  13. Mackin Scale 0 – rare 1 – very light 2 – light 3 – moderate 4 – moderate/heavy 5 – heavy

  14. RFTM results false wintered no false winter 72% prevalence 68% prevalence weighted prevalence: weighted prevalance: 1.28 (n=25) 1.32 (n=25 No difference between treatments

  15. Update on status of VIMS research hatchery No planning until: July 1, 2017 / Start building: July 1, 2018

  16. building concept concept views nort h

  17. building concept concept views

  18. design . entrance perspective

  19. int erior design. interior perspectives- lobby

  20. int erior design. interior perspectives- transition

  21. int erior design. interior perspectives- lower level Interior Perspectives

  22. int erior design. interior perspectives- lower level

  23. revised f irst second plan . programming floor plans- level 2

  24. revised f irst f loor plan . programming floor plans- level 1

  25. North side

  26. South side

  27. Hatchery floor

  28. Questions about the planning process What role industry? • Determining research capabilities • e.g., carbonate chemistry? • e.g., conditioning studies? • e.g., closed systems? Research hatchery manager? • Oversee facility, part of AQ Program • PI for hatchery related research • “Journeyman” with experience • e.g., Alan Barton Expertise in design? • VA industry? • Other sources of expertise • Donaldson • Meritt • Davis

  29. Family deployment 2016

  30. 2N Deployment 2016 • 49 low salinity families – 25 also deployed in York River* • 50 high salinity families – 15 also deployed in Lewisetta* * some families perform well in both environments • long line – York River & Cambridge • rack & bag – Lynnhaven & Lewisetta

  31. 2N Deployment 2016 • Hungars Creek – Cherrystone – 30 families (15 high, 15 low, 2 reps per) – rack and bag – Do ABC families perform differently in the creek environments of the Eastern Shore? • loggers in place to measure temp. & salinity

  32. 3n mortality research update Grad student: Joseph Matt Post doc: Eric Guévélou

  33. “The relationship between reproduction and mortality in triploid Crassostrea virginica : a matter of economic importance” Spawned in March 2015 – all groups now “ideal” size tetraploid diploid male 4n VA 4n LA 2n VA 2n ME 2n VA VVV LLV VV MV female 2n ME VVM LLM VM MM 4 triploid groups 4 diploid “controls”

  34. Deployment sites Control

  35. Data from triploid mortality 2016 • Triploid reproductive effort • Environment • Physiology • Growth • Mortality • Condition • NIRS • Glycogen • (Lipid)

  36. Microbiome – BK Song, Allen, Carnegie Profiling the microbiome composition of hatchery reared oyster larvae Sea Grant PD funds Summer 2015 The totality of microorganisms and their collective genetic material present in a particular environment.

  37. Sampling ontogeny across hatcheries

  38. Sampling seasonal variation across hatcheries march april may june august july GP KAC OSH WOC

  39. 0.3� D-3 D-2 V-3 D-1 0.2� PC1 - Percent Variation Explained – 7.9% D-3 D-1 GP V-2 KAC V-2 0.1� D-2 D-3 PV-3 PV-2 WOC V-3 V-1 D-2 0� D-3 -0.4� PV-3 -0.3� -0.2� -0.1� 0� 0.1� 0.2� 0.3� D-1 V-1 D-1 V-1 -0.1� PV-1 PV-2 V-1 PV-3 PV-1 -0.2� D-2 V-3 PV-2 OSH -0.3� V-2 -0.4� V-3 -0.5� PC1 - Percent Variation Explained – 9.7%

  40. D-stage V-stage Predominant genus found in adult PV-stage

  41. New crop of OATs Other training

  42. 2016 OATs (32 applicants) 3 ‘regular’ OATs • Imani Black • Track record with Bay interests • Lacrosse star, ODU • Graduates May ‘16 • Lizzy Profita • Experience in shellfish, algae • Americorps – Cape Cod shellfish • Prefers field to lab • Haley Ladeau • Experience at Milford • Experience at AIC (Rutgers, Cape May) • Extrovert! 1 ‘research assistant’ OAT • Chris Rubino • Internships at several hatcheries • Familiar with the field • Not afraid of “unflattering jobs”

  43. 2016 OAT applicants (placements) • Nick Booth – Cherrystone intern • Carlos Santos – Mobjack Bay Seafood • James McCarthy – Lynnhaven • Ellis Chapman – Cherrystone • Diamond in the rough • Gretchen Davis – Northern Neck

  44. 2016 OAT Jr (test program) MathScience Innovation Center, Richmond • 2 week summer aquaculture course – “Tank to Table” • Tilapia • Trout • Oysters • Select four top students (work ethic) – oyster internship • 2 to VIMS for one week • Another 2 to VIMS for one week

  45. VIMS Aquaculture Program Industry Advisory Committee redefined?

  46. Succession at ABC (first shown June 2016) pre-2015 2015 “five” years FTE Aquaculture @ VIMS Stan Stan Jess (asst. director) Jess Jess GP KAC 2n KAC ABC field field 2n 4n GP 4n

  47. INFRASTRUCTURE – VIMS Aquaculture Program AP Director • Brings research interest • Coordinates aquaculture research at VIMS • Administers rapid response funds Ryan Carnegie Kim Reece ABC Iris Anderson BK Song Juliette Smith Elizabeth Shadwick Jan McDowell Roger Mann

  48. ENDOWMENT – VIMS Aquaculture Program $$ $ AP Director • Brings research interest • Coordinates aquaculture research at VIMS $ • Administers rapid response funds Discretionary funding Ryan Carnegie Kim Reece ABC Iris Anderson BK Song Juliette Smith Elizabeth Shadwick Jan McDowell Roger Mann

  49. Shellfish Industry Advisory Committee – VIMS Aquaculture Program $$ $ AP Director • Brings research interest • Coordinates aquaculture research at VIMS $ • Administers rapid response funds Discretionary funding Ryan Carnegie Kim Reece ABC Iris Anderson BK Song Juliette Smith Elizabeth Shadwick Jan McDowell Roger Mann

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