Agenda Starmerella bombicola and sophorolipids 02 Integrated - - PowerPoint PPT Presentation

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Agenda Starmerella bombicola and sophorolipids 02 Integrated - - PowerPoint PPT Presentation

Apr 10, 2023 201 likes •637 views

01 Introduction Agenda Starmerella bombicola and sophorolipids 02 Integrated bioprocess design: a case study for Starmerella bombicola Strain engineering, process development & -scale up and application research 01. Introduction

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01 Introduction

Agenda

02 Integrated bioprocess design: a case study for Starmerella bombicola

Strain engineering, process development & -scale up and application research Starmerella bombicola and sophorolipids

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01.

Introduction

Starmerella bombicola and sophorolipids

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Introduction: Starmerella bombicola

01.

Starmerella bombicola

Source: www.first-nature.com Source: www.wildlifetrusts.org

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Introduction: Starmerella bombicola

01.

Starmerella bombicola

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Introduction: Starmerella bombicola

01.

Starmerella bombicola

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  • Produced by yeasts
  • Low foaming surfactant
  • Mild antimicrobial properties (anti acne, anti odor,…),
  • Antiviral, - cancer, …
  • Applications: detergents and cosmetics

>> Sophorolipids Glycolipids

F e r m e n t a t i o n ( M i c r o b i a l ) Source: www.rewoferm.evonik.com Source: www.athinarom.gr Source: www.ecover.com Source: www.wheatoleo.com

Introduction: SLs: commercial reality

01.

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  • Acidic or lactonic form

Introduction: Sophorolipids a complex mix

01.

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  • Acidic or lactonic form
  • Acetylation: 0, 1 or 2 groups

Introduction: Sophorolipids a complex mix

01.

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  • Acidic or lactonic form
  • Acetylation: 0, 1 or 2 groups
  • Fatty acid chain length: C16-C18
  • Unsaturated bounds: 0, 1 or 2

Introduction: Sophorolipids a complex mix

01.

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  • Acidic or lactonic form
  • Acetylation: 0, 1 or 2 groups
  • Fatty acid chain length: C16-C18
  • Unsaturated bounds: 0, 1 or 2
  • Position of hydroxylation: ω of ω-1

Introduction: Sophorolipids a complex mix

01.

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“Variation is desired across (1) products, preferably not IN (2) products”

  • Acidic or lactonic form
  • Acetylation: 0, 1 or 2 groups
  • Fatty acid chain length: C16-C18
  • Unsaturated bounds: 0, 1 or 2
  • Position of hydroxylation: ω of ω-1

Introduction: Sophorolipids a complex mix

01.

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Variation Cheap Uniform Pure

Solution: Integrated Bio process Design to bring IB to market Market demands Sophorolipids

...

Introduction: disadvantages of sophorolipids

01.

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Integrated bioprocess design: a case study for S. bombicola

Genetic engineering, process development & scale up and application research

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02.

A case study for BS production with S. bombicola Genetic Engineering Process development & scale up Integrated bioprocess design approach (IBD) (New-to-nature) sophorolipids

Uniform Pure Cheap Uniform Variation Cheap

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02.

A case study for BS production with S. bombicola Genetic Engineering Integrated bioprocess design approach (IBD) (New-to-nature) sophorolipids

Uniform Variation Cheap

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  • S. bombicola wild type

sophorolipids

  • 02. IBD approach: strain engineering
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Acidic SLs Lactonic SLs Battery of new S. bombicola strains 100 % 100 %

Increasing uniformity:

  • 02. IBD approach: strain engineering
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02.

Increasing variety

  • 02. IBD approach: strain engineering
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02.

A case study for BS production with S. bombicola Genetic Engineering Integrated bioprocess design approach (IBD) (New-to-nature) sophorolipids

Uniform Variation Cheap

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02.

A case study for BS production with S. bombicola Genetic Engineering Process development & scale up Integrated bioprocess design approach (IBD) (New-to-nature) sophorolipids

Uniform Pure Cheap Uniform Variation Cheap

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02.

  • 02. IBD approach: Process Development
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Bolaform Sophorolipids

Overall productivity lower as wild type

  • 02. IBD approach: Process Development
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Bolaform Sophorolipids

Overall productivity lower as wild type

  • Strain improvement
  • 02. IBD approach: Process Development
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Bolaform Sophorolipids

Overall productivity lower as wild type

  • Strain improvement
  • Process development

IBD approach: Process Development

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Bolaform Sophorolipids

Overall productivity lower as wild type

  • Strain improvement
  • Process development

FERMENTATION: ° Standard conditions (0.05 g/L.h) ° Medium optimization (0.22 g/L.h) ° Substrate optimization (0.44 g/L.h) ° Fermentation optimization (0.72 g/L.h)

02.

IBD approach: Process Development

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Bolaform Sophorolipids

Overall productivity lower as wild type

  • Strain improvement
  • Process development

DOWNSTREAM PROCESSING: ° Purity (85 % to 95 %) ° Yield/Recovery (70 % to 98 %)

02.

IBD approach: Process Development

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Bolaform Sophorolipids

Overall productivity lower as wild type

  • Strain improvement
  • Process development
  • Scale up

7 L => 100 L => 15 000 L:

02.

IBD approach: Scale up

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02.

IBD approach: Scale up

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Bolaform Sophorolipids

  • Strain improvement
  • Process development
  • Scale up

7 L => 100 L => 15 000 L: ° Generate data (TEA and LCA)

02.

IBD approach: Scale up

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Bolaform Sophorolipids

  • Strain improvement
  • Process development
  • Scale up

7 L => 100 L => 15 000 L: ° Generate data (TEA and LCA) ° Generate samples (kg to ton scale)

02.

IBD approach: Scale up

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Bolaform Sophorolipids

  • Strain improvement
  • Process development
  • Scale up
  • Application research

02.

IBD approach: Application research

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v 19%

12%

7%

52%

6%

4%

H o u s e h o l d d e t e r g e n t s O t h e r s ( p a i n t s & i n k s , o i l f i e l d c h e m i c a l s , t e x t i l e s , . . . ) P e r s o n a l C a r e I n d u s t r i a l c l e a n e r s F o o d p r o c e s s i n g A g r i c u l t u r a l c h e m i c a l s

02.

IBD approach: Application research

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v 19%

12%

7%

52%

6%

4%

H o u s e h o l d d e t e r g e n t s O t h e r s ( p a i n t s & i n k s , o i l f i e l d c h e m i c a l s , t e x t i l e s , . . . ) P e r s o n a l C a r e I n d u s t r i a l c l e a n e r s F o o d p r o c e s s i n g A g r i c u l t u r a l c h e m i c a l s

02.

IBD approach: Application research

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Bolaform Sophorolipids

  • Strain improvement
  • Process development
  • Scale up
  • Application testing

° Emulsification ° Wetting ability ° Film formation / contact angle ° Anti scaling properties ° Foaming ° CMC ° Stability

02.

IBD approach: Application research

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Bolaform Sophorolipids

  • Strain improvement
  • Process development
  • Scale up
  • Application testing

° Emulsification ° Wetting ability ° Film formation / contact angle ° Anti scaling properties ° Foaming ° CMC ° Stability

02.

IBD approach: Application research

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Bolaform Sophorolipids

  • Strain improvement
  • Process development
  • Scale up
  • Application testing

° Emulsification ° Wetting ability ° Film formation / contact angle ° Anti scaling properties ° Foaming ° CMC ° Stability

02.

IBD approach: Application research

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Stability

02.

IBD approach: Application research

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Bolaform Sophorolipids

  • Strain improvement
  • Process development
  • Scale up
  • Application testing

° Emulsification ° Wetting ability ° Film formation / contact angle ° Anti scaling properties ° Foaming ° CMC ° Stability

02.

IBD approach: Application research

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Bolaform Sophorolipids

Stability issues with bola sophorolipids

  • Strain improvement

° create a new strain producing stable bola’s

02.

IBD approach: Application research

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Bolaform Sophorolipids

02.

IBD approach: Application research Stability issues with bola sophorolipids

  • Strain improvement

° create a new strain producing stable bola’s

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  • 02. IBD approach: an iterative process

IPD-to bring microbial biosurfactants to market

Strain engineering

SCALE UP

Application 3 Application 1 Application 2 Application 4

IBD approach to bring bola SLs to market

COMMERCIALISATION Of BOLA SOPHOROLIPIDS Fermentation Development Purification Development

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02.

IBD approach: projects ° FP7 project Biosurfing ° FP7 project IB2Market ° IWT-IM project Stabosurf ° BBI-H2020 project Carbosurf ° Vlaio Intercluster project AppliSurf ° H2020 project Marisurf

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Thank you!

w w w. u ge nt. b e http :/ / in b io. b e w w w. b b e u . org