5 MELANOSTATINE FOCUSING ON WHITENING Mechanism of action - PowerPoint PPT Presentation

SKIN RADIANCE / SKIN LIGHTENING – WHITENING / ANTI-DARK SPOT ™ 5 MELANOSTATINE FOCUSING ON WHITENING

• Mechanism of action • Antagonist effect of Melanostatine ™ 5 on camp • Inhibition of Tyrosinase • Inhibition hyper-stimulation of melanin • Reversing hyper-pigmentation • Lightening improvement in Asian skin • Cosmetics properties • Features and benefits • Applications • Formulation

Mechanism of action  a-MSH is the natural substance produced by our body which is responsible for several physiological activities like pigmentation.  Tyrosinase is stimulated by the bonding of a-MSH on its specific receptor MC1-R.  The stimulation of the tyrosinase activity, induces the hydroxylation of the amino acid tyrosine to DOPA and catalyses its oxidation to DOPA-quinone.  The final step is the synthesis of melanin in organelles called melanosomes, in melanocytes.

Mechanism of action Decreases the melanin synthesis and blocks the melanin hyper-production through the inhibition of the MC1-R The ideal skin lightening agent for cosmetic products is one that inhibits melanogenesis without cytotoxicity .

Antagonist effect of Melanostatine ™ 5 on camp IC50 of Melanostatine ™ 5 is 3.7nM Melanostatine ™ 5 antagonizes the signal transduction induced by 10-7M a-MSH

Inhibition of Tyrosinase The tyrosinase activity is detected with a Western blot. Tyrosine is labelled by specific anti-tyrosinase monoclonal antibody. Melanostatine ™ 5 blocks the melanogenesis pathway and inhibits tyrosinase activity and melanin synthesis

Inhibiting hyper-stimulation of melanin The total proteins content + melanin synthesis were determined by the colorimetric method Pierce BCA (Bicinchoninic Acid) Protein Assay Melanostatine ™ 5 inhibits the synthesis of melanin induced by α -MSH by 33%

Reversing hyper-pigmentation α -MSH : numerous highly DOPA- positive melanocytes α -MSH + MELANOSTATINE ™ 5: less DOPA-positive melanocytes These microscope images demonstrate that Melanostatine ™ 5 does not interfere with the melanocyte’s functions. However when melanocytes are stimulated, Melanostatine ™ 5 opposes the hyperproduction of melanin.

Lightening improvement in Asian skin Test protocol 50 Asian (female) volunteers applied a whitening cream containing 2% Tyrostat TM - 11 and 4% Melanostatine ™ 5, 2x/day for 4 weeks. With complementary and synergic pathways, TyrostatTM-11 and Melanostatine ™ 5 reduce sun spots, freckles and uneven pigmentation allowing a lighter and luminous skin tone.

Cosmetic Properties  Decreases a-MSH induced melanin synthesis  Reduces cutaneous hyper pigmentation New concept in the inhibition of Melanogenesis Hyper-Stimulation NEW GENERATION OF CUTANEOUS WHITENER-LIGHTENER

Features and benefits

Product information Recommended dosage: 4 – 8% Potential Applications: • Lightening care • Photoaging • Whitening • Ethnic skin care • Skin tone equalizer • Hand cream • Aging spot corrective care • Reducing brown spots cream • Skin illumination-brilliance (foundation-powder) Incorporation: at the end of the formulation (35/40 ˚C), pH:5-7 INCI name: Water (and) Dextran (and) Nonapeptide-1

Formulation