5 MELANOSTATINE FOCUSING ON WHITENING Mechanism of action - - PowerPoint PPT Presentation

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5 MELANOSTATINE FOCUSING ON WHITENING Mechanism of action - - PowerPoint PPT Presentation

Sep 17, 2022 309 likes •447 views

SKIN RADIANCE / SKIN LIGHTENING WHITENING / ANTI-DARK SPOT 5 MELANOSTATINE FOCUSING ON WHITENING Mechanism of action Antagonist effect of Melanostatine 5 on camp Inhibition of Tyrosinase Inhibition

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SLIDE 1

MELANOSTATINE

™5

FOCUSING ON WHITENING

SKIN RADIANCE / SKIN LIGHTENING – WHITENING / ANTI-DARK SPOT

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SLIDE 2
  • Mechanism of action
  • Antagonist effect of Melanostatine™5 on camp
  • Inhibition of Tyrosinase
  • Inhibition hyper-stimulation of melanin
  • Reversing hyper-pigmentation
  • Lightening improvement in Asian skin
  • Cosmetics properties
  • Features and benefits
  • Applications
  • Formulation
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SLIDE 3

Mechanism of action

  • a-MSH is the natural substance produced by our body which is responsible for

several physiological activities like pigmentation.

  • Tyrosinase is stimulated by the bonding of a-MSH on its specific receptor MC1-R.
  • The stimulation of the tyrosinase activity, induces the hydroxylation of the amino

acid tyrosine to DOPA and catalyses its oxidation to DOPA-quinone.

  • The final step is the synthesis of melanin in organelles called melanosomes, in

melanocytes.

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SLIDE 4

Mechanism of action

Decreases the melanin synthesis and blocks the melanin hyper-production through the inhibition of the MC1-R

The ideal skin lightening agent for cosmetic products is one that inhibits melanogenesis without cytotoxicity .

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SLIDE 5

Antagonist effect of Melanostatine™5 on camp

IC50 of Melanostatine™5 is 3.7nM

Melanostatine™5 antagonizes the signal transduction induced by 10-7M a-MSH

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SLIDE 6

Inhibition of Tyrosinase

The tyrosinase activity is detected with a Western blot. Tyrosine is labelled by specific anti-tyrosinase monoclonal antibody. Melanostatine™5 blocks the melanogenesis pathway and inhibits tyrosinase activity and melanin synthesis

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SLIDE 7

Melanostatine™5 inhibits the synthesis of melanin induced by α-MSH by 33%

Inhibiting hyper-stimulation of melanin

The total proteins content + melanin synthesis were determined by the colorimetric method Pierce BCA (Bicinchoninic Acid) Protein Assay

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SLIDE 8

α-MSH : numerous highly DOPA- positive melanocytes α-MSH + MELANOSTATINE™5: less DOPA-positive melanocytes

Reversing hyper-pigmentation

These microscope images demonstrate that Melanostatine™5 does not interfere with the melanocyte’s functions. However when melanocytes are stimulated, Melanostatine™5 opposes the hyperproduction of melanin.

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SLIDE 9

Test protocol 50 Asian (female) volunteers applied a whitening cream containing 2% TyrostatTM- 11 and 4% Melanostatine™5, 2x/day for 4 weeks.

Lightening improvement in Asian skin

With complementary and synergic pathways, TyrostatTM-11 and Melanostatine™5 reduce sun spots, freckles and uneven pigmentation allowing a lighter and luminous skin tone.

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SLIDE 10

New concept in the inhibition of Melanogenesis Hyper-Stimulation

  • Decreases a-MSH induced melanin synthesis
  • Reduces cutaneous hyper pigmentation

Cosmetic Properties

NEW GENERATION OF CUTANEOUS WHITENER-LIGHTENER

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SLIDE 11

Features and benefits

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SLIDE 12

Recommended dosage: 4 – 8% Potential Applications: Incorporation: at the end of the formulation (35/40˚C), pH:5-7 INCI name: Water (and) Dextran (and) Nonapeptide-1

  • Lightening care
  • Whitening
  • Skin tone equalizer
  • Aging spot corrective care
  • Photoaging
  • Ethnic skin care
  • Hand cream
  • Reducing brown spots cream
  • Skin illumination-brilliance

(foundation-powder)

Product information

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SLIDE 13

Formulation