2 Yes! s! eYE YE DO DO. 3 Yes! s! 4 Or Oral C l Canc ncer All - - PowerPoint PPT Presentation

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Yes! s! eYE YE DO DO.

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Yes! s!

Or Oral C l Canc ncer

www.dentaltribune.com/articles/news/americas/17019_byproducts_of_periodontal_bacteria_may_induce_oral_cancer_growth.html

http://www.moderncancerhospital.com/cancer/oral-cancer/

• All malignant

cancerous tissue growth located in the

• ral cavity
• 90% are squamous

cell carcinomas

https://en.wikipedia.org/wiki/Oral_cancer

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acruisingc uisingcouple

• uple.c

.com

• m/2013/07/hik

hiking-te ing-teapot-m pot-mounta

• untain-a

in-and- nd-mt mt-ba banping nping/

World Cancer Rank

• f diagnosed in 2012
• No. 1

. 15 Lip Lip, or , oral c l cavity vity

Mortality rate changes of major cancers in Taiwan, 2002 vs. 2012

 Most patients present advanced stages of OSCC at

the time of diagnosis

 Early detection is important for OSCC patients

Taiwan cancer registry database 2004-2010

5 10 15 20 25 30 35 40 45

¡Percentage (%) Stage Stages at the time of diagnosis

1 28 21 12 12 39 39

10 20 30 40 50 60 70 80 90

¡Five-year survival rate

80 76 68 52 52 33 33

0 I II III IV 0 I II III IV

Stage

Epide Epidemiology iology of

• f or
• ral c

l cavity vity squa squamous c

• us cell c

ll carcinom inoma (OSC (OSCC) in ) in Taiw iwan

• Improve survival
• Lower cost of medical treatment

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A rapid test device

Im Impor porta tanc nce of

• f Ea

Early ly Dete tection tion

Or Oral C l Canc ncer

http://collect.life.com.tw/note.php?action=index&sid=15119 https://openclipart.org/detail/195993/red-open-mouth

Early Detection Health Education

Visiting isiting Dir irector of tor of R Radia diation tion Onc Oncology

• logy

Cancer specialist

Nowadays, oral cancers are detected mostly by direct observation

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Inte Intervie view with a w with an Or n Oral C l Canc ncer P r Patie tient nt

Sunshine Social Welfare Foundation

Why potential oral cancer patients refuse further evaluation

• 50% fear false detection
• 70% refuse in

invasiv sive biopsy biopsy

too subje too subjectiv tive

A ¡rapid ¡test ¡device

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Non-­‑invasive Objec4ve

How ¡do ¡we ¡achieve ¡early ¡ detec4on ¡

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Str Strate tegy: T gy: Toe

• ehold Switc

hold Switch a h as R s RNA Se Sensor nsor

• Cell. 2014 6;159(4):925-39

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Ribosome

Toe

• ehold Switc

hold Switch D h Design sign

4 mRNA unique sequences (30 nts)

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RNA structure prediction

IL-8

Trigger

Result sult

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50 100 150 200 250 300 350 SAT DUSP1 IL8

O.D .D.5 .595nm nm (X1 (X106) Toe

• ehold s

hold switc witch ta h target

Switch only Switch+ trigger

Toehold Switch in vivo Characterization

Triggers (IL-8, IL-1β, SAT and DUSP1) 4 Toehold Switches Luciferase

RNA D Dete tection D tion Devic vice Sum Summary ry

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Engine Engineered Y d Yeast D st Dete tecting IL-8 ting IL-8

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Biobric iobrick of

• f Engine

Engineered d Yeast st

Rhodopsin pe hodopsin peptide ptide se seque quenc nce

Rhodopsin carries CXCR1 to the plasma membrane of the yeast

CXCR1 se seque quenc nce

CXCR1: engineered receptor of IL-8 presented on the yeast plasma membrane

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GFP ¡mRNA

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Result sult

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Devic vice

Devic vice

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Biose iosensor nsors Sa Saliv liva Inle Inlet

Stir biose Stir biosensor nsors with sa s with saliv liva sa sample ples

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Future Work

• Our toehold switch design has the

potential to be used to detect any mRNA biomarkers

• The design of our yeast-based

biosensor could be applied to detect

• ther ligands
• Our construct may be developed into a

commercial kit

Human Practices

• Global Survey

about Oral Cancer

• Promote Synthetic

Biology & Our Project

• Consult Doctor & Patient
• Raise Oral Cancer

Awareness

Human Practices

• Global Survey

about Oral Cancer

• Promote Synthetic

Biology & Our Project

• Consult Doctor & Patient
• Raise Oral Cancer

Awareness

Etiology of Oral Cancer

 Chewing betel nuts  Smoking  Alcoholism  Eating hot and spicy food often  French kiss with HPV patient  Oral sex with HPV patient  Decayed tooth  Genes 26

10 20 30 40 50 60 70 80 90 100 Chewing betal nuts Smoking Alcoholism Eating hot and spicy food often French kiss with HPV patient Oral sex with HPV patient Decayed tooth Genes

% of respondent who chose the answer

in CGU (n=100) in Public (n=112)

Wha What do y t do you think

• u think ar

are the the

CAUSES SES for or

• r oral c

l canc ncer? r?

http://www.cancer.org/cancer/oralcavityandoropharyngealcancer/detailedguide/oral-cavity-and-oropharyngeal-cancer-risk-factors

Achie hievements nts

 15 biobricks including new basic parts and composite parts complete.  Prove the function of Rho-CXCR1 biobrick (BBa_K1728024) works in yeast.  Achieve policy and practice through giving out questionnaires include international questionnaires, health education.

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Acknowle nowledg dgements nts

Sz Sze-C

• Che

heng J ng John Lo

• hn Lo,

, Ph.D Ph.D Shu-Y Shu-Yua uan Y n Yang ng, , Ph.D Ph.D Hsin-P sin-Pai Li, PhD Li, PhD Sc Scott C

• tt C. Sc

. Schuyle huyler, , Ph.D Ph.D Yu-Sun C u-Sun Cha hang ng, , Ph.D Ph.D Gua Guang ng-W

• Wu C

u Che hen, n, Ph.D Ph.D Kin F in Fong Le

• ng Lei, PhD

i, PhD Cha hao-Sung La

• -Sung Lai,

i, Ph.D Ph.D Integrated DNA Technologies

Thank you for your listening.

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