2 Yes! s! eYE YE DO DO. 3 Yes! s! 4 Or Oral C l Canc ncer All - PowerPoint PPT Presentation

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Yes! s! eYE YE DO DO. 3

Yes! s! 4

Or Oral C l Canc ncer • All malignant cancerous tissue growth located in the oral cavity • 90% are squamous cell carcinomas https://en.wikipedia.org/wiki/Oral_cancer http://www.moderncancerhospital.com/cancer/oral-cancer/ www.dentaltribune.com/articles/news/americas/17019_byproducts_of_periodontal_bacteria_may_induce_oral_cancer_growth.html

Mortality rate changes of major cancers in Taiwan, 2002 vs. 2012 World Cancer Rank of diagnosed in 2012 No. 1 . 15 Lip, or Lip , oral c l cavity vity 6 acruisingc uisingcouple ouple.c .com om/2013/07/hik hiking-te ing-teapot-m pot-mounta ountain-a in-and- nd-mt mt-ba banping nping /

Epide Epidemiology iology of of or oral c l cavity vity squamous c squa ous cell c ll carcinom inoma (OSC (OSCC) in ) in Taiw iwan  Most patients present advanced stages of OSCC at the time of diagnosis  Early detection is important for OSCC patients ¡ Five-year survival rate Stages at the time of diagnosis 45 90 80 39 39 76 ¡ Percentage (%) 40 80 68 35 70 28 30 60 52 52 25 50 21 20 33 33 40 12 12 15 30 10 20 5 10 1 0 0 0 I II III IV 0 I II III IV Stage Stage Taiwan cancer registry database 2004-2010

Im Impor porta tanc nce of of Ea Early ly Dete tection tion • Improve survival • Lower cost of medical treatment A rapid test device 8 ¡

Or Oral C l Canc ncer Health Education Early Detection https://openclipart.org/detail/195993/red-open-mouth http://collect.life.com.tw/note.php?action=index&sid=15119

nt Inte Intervie view with a w with an Or n Oral C l Canc ncer P r Patie tient Visiting isiting Dir irector of tor of R Radia diation tion Sunshine Social Welfare Foundation Oncology Onc ology Nowadays, oral cancers Cancer specialist Why potential oral cancer are detected mostly by patients refuse further direct observation evaluation • 50% fear false detection too subje too subjectiv tive • 70% refuse in invasiv sive biopsy biopsy 10

How ¡do ¡we ¡achieve ¡early ¡ detec4on ¡ Non-­‑invasive Objec4ve A ¡rapid ¡test ¡device 11

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Str Strate tegy: T gy: Toe oehold Switc hold Switch a h as R s RNA Se Sensor nsor Ribosome 13 ¡ Cell. 2014 6;159(4):925-39

Toe oehold Switc hold Switch D h Design sign 4 mRNA unique RNA structure sequences (30 nts) prediction Trigger IL-8 14 ¡

Result sult Toehold Switch in vivo Characterization 350 Switch only 300 Switch+ trigger (X10 6 ) 250 nm (X1 200 .595nm 150 .D.5 O.D 100 50 0 SAT DUSP1 IL8 Toe oehold s hold switc witch ta h target 15 ¡

RNA D Dete tection D tion Devic vice Sum Summary ry Triggers (IL-8, IL-1 β , SAT 4 Toehold Switches Luciferase and DUSP1) 16 ¡

Engine Engineered Y d Yeast D st Dete tecting IL-8 ting IL-8 17

Biobric iobrick of of Engine Engineered d Yeast st CXCR1 se seque quenc nce Rhodopsin pe hodopsin peptide ptide se seque quenc nce CXCR1: engineered Rhodopsin carries CXCR1 to the receptor of IL-8 plasma membrane of the yeast presented on the yeast plasma membrane 18

GFP ¡mRNA 19

Result sult 20

Devic vice 21

Devic vice Biose iosensor nsors Stir biosensor Stir biose nsors with sa s with saliv liva sa sample ples Sa Saliv liva Inle Inlet 22

Future Work • Our toehold switch design has the potential to be used to detect any mRNA biomarkers • The design of our yeast-based biosensor could be applied to detect other ligands • Our construct may be developed into a commercial kit 23 ¡

Human Practices • Global Survey about Oral Cancer • Promote Synthetic Biology & Our Project • Consult Doctor & Patient • Raise Oral Cancer Awareness

Human Practices • Global Survey about Oral Cancer • Promote Synthetic Biology & Our Project • Consult Doctor & Patient • Raise Oral Cancer Awareness

Etiology of Oral Cancer  Chewing betel nuts  Smoking  Alcoholism  Eating hot and spicy food often  French kiss with HPV patient  Oral sex with HPV patient  Decayed tooth  Genes 26

What do y Wha t do you think ou think ar are the the CAUSES SES for or or oral c l canc ncer? r? % of respondent who chose the answer 100 in CGU (n=100) in Public (n=112) 90 80 70 60 50 40 30 20 10 0 Chewing Smoking Alcoholism Eating hot French kiss Oral sex with Decayed Genes betal nuts and spicy with HPV HPV patient tooth food often patient http://www.cancer.org/cancer/oralcavityandoropharyngealcancer/detailedguide/oral-cavity-and-oropharyngeal-cancer-risk-factors

Achie hievements nts  15 biobricks including new basic parts and composite parts complete.  Prove the function of Rho-CXCR1 biobrick (BBa_K1728024) works in yeast.  Achieve policy and practice through giving out questionnaires include international questionnaires, health education.

Acknowle nowledg dgements nts Ph.D Gua Ph.D Li, PhD Yu-Sun C u-Sun Cha hang ng, , Ph.D Guang ng-W -Wu C u Che hen, n, Ph.D Hsin-P sin-Pai Li, PhD Sz Sze-C -Che heng J ng John Lo ohn Lo, , Ph.D Ph.D � Integrated DNA Technologies Ph.D i, PhD Ph.D Scott C Sc ott C. Sc . Schuyle huyler, , Ph.D Shu-Yua Shu-Y uan Y n Yang ng, , Ph.D Ph.D Kin F in Fong Le ong Lei, PhD Cha hao-Sung La o-Sung Lai, i, Ph.D 29 ¡

Thank you for your listening. 30 ¡