Phylogenetics 1: An overview Phylogenetics 1: An overview Phylogenetic tree used in The Origin of Species . Darwin wasn’t just thinking about “ The affinities of all beings of the same class have classification based on phylogenies. He used them to visualize the process of divergence within species and the splitting of populations into separate species. Darwin sometimes been represented by a great tree. I believe this used this figure to illustrate divergence of variants within species; over time successively more variation accumulates. Eventually some of this variation forms the basis for new species. simile largely speaks the truth. The green budding twigs may represent existing species; and those produced during former years may represent the long succession of extinct species...and this connection of the former and present buds by ramifying branches may well represent the classification of all extinct and living species in groups subordinate to groups. ” Charles Darwin, in Chapter IV of On the origin of species by means of natural selection, or the preservation of favoured races in the struggle for life. Unrooted tree diagram drawn in the margin of one of Charles Darwin’s notebooks 1
Phylogenetics: The biological discipline devoted to reconstructing, gene or genome phylogenies Growth of phylogenetics: 1. Phylogenetic methods (1960’s) 2. Recognition that phylogenies were relevant to nearly all disciplines of biology (1970’s?) 3. Molecular biotechnology revolution [PCR] (1980’s) 4. Economics of computational capacity (1990’s) Phylogenetics 1: An overview clade Scale bar 0.1 2
Phylogenetics 1: An overview • Analogy • Homology • Polarity • Ancestral character • Derived character Phylogenetics 1: An overview Branch lengths estimated under the assumption of the Branch lengths estimated without assumption of the molecular molecular clock clock Felis Felis Canis Canis Ursus Ursus Root Bos Bos Hippopotamus Hippopotamus Physeter Physeter Balaenoptera Balaenoptera Rhinoceros Rhinocero s 0.1 Equus Equus 0.1 Tips are contemporary; the distance Tips are NOT contemporary; the distance from root to each tip is the same from root to each tip is NOT the same 3
The phylogenetic comparative method Hypothetical dataset for phenotype (Y) Two point dataset from early in and ecological variable (X) evolutionary history Y Y X X Hypothetical example: Y: size of a primates big toe X: The stubbiness of the habitat The phylogenetic comparative method Hypothetical dataset with points Phylogeny of two groups of close relatives coloured according to clade of origin “Big-toe clade” “Little-toe clade” Y Recent diversifications X “Little-toed” clade “Big-toed” clade Old divergence of “big-toed” and “little-toed” primates Species are NOT drawn independently from the same distribution. “ phylogenies are fundamental to comparative biology; there is no doing it without taking them into account ” ⎯ Joseph Felsenstein 4
Applications of phylogenetics 1. Sytematics, classification, and taxonomy 2. Biogeography 3. Health Sciences 4. Agriculture 5. Conservation 6. Linguistics Applications of phylogenetics: systematics E RNST H AECKEL ’ S “T REE OF L IFE ”, DRAWN SOMETIME IN THE LATE 1800’ S Placed Menschen (“Men”) at the “top” of the tree among the Affen (“Apes”). Haeckle was first to suggest man’s ancestry was among the Great Apes. This tree was a tree of “men”, and Haeckels’s placement of Menschen at the top was intentional This tree and associated system of classification is different from modern ones Non-mammalian in that it is based on the vertebrates notion of linear progress (like a ladder) from the most primitive single-celled organisms “upwards” to man (at the very top). Haeckel considered the things near the top as “more evolved” and things near Invertebrates the bottom as “primitive”. Ernst Haeckel (1834-1919) was a German biologist and scientific illustrator. He was one of the first popularizers of Darwin’s Theory of Evolution. The tree to the Protozoa left is from his book “ General Morphology – founded on the descent theory ”. 5
Applications of phylogenetics: systematics Monophyly, paraphyly and polyphyly A B C D E D E A B C F F H H G G J J Monophyletic group Paraphyletic group (AHJGFDE) and a polyphyletic group (BC) [Clade] Applications of phylogenetics: systematics The old Reptilia as an example of classification based on a paraphyletic group. Aves (birds) Old Reptilia is a GRADE Lots of dinosaur diversity Ornithischia (some plant eating dinosaurs) Crocodylomorph (gators and crocs) Amniota is a clade Lepidosauromorph (lizards snakes, etc.) Anapsids (turtles and relatives) I am a synapsid too! Diversity of extinct mammal-like reptiles Mammals (Synapsids) 6
Applications of phylogenetics: systematics http://www.tolweb.org/tree/ Applications of phylogenetics: biogeography Phylogeorgaphy allows one to test hypotheses such as whether geographic/ environmental factors have been historically important barriers to gene flow. WEST: low elevation and dry EAST: high elevation and wet 7
Applications of phylogenetics: biogeography of mouse lemurs Applications of phylogenetics: biogeography of mouse lemurs Phylogeographic analysis of mouse lemurs contradicts the expected east-west disjunction for Madagascar, and suggests a completely novel north-south disjunction. The observed phylogenetic tree was inferred from mitochondrial DNA gene sequences. Figure adapted from separate figures in A. D. Yoder (2004) In press 8
Applications of phylogenetics: biogeography of mouse lemurs Applications of phylogenetics: biogeography ⇒ conservation 9
Applications of phylogenetics: Ann Yoder’s research group Applications of phylogenetics: Health Sciences and HIV 10
Applications of phylogenetics: Health Sciences and HIV HIV transmission in health care: 1. Patient ⇒ health care worker: well known 2. Health care worker ⇒ patient: unknown CDC: epidemiological investigation of dentist with infected patients in 1990’s • only risk factor was a common dentist • phylogenetics of HIV env gene sequences HIV-1 genome: HIV-1 genome: Applications of phylogenetics: Health Sciences and HIV 11
Applications of phylogenetics: Health Sciences and HIV Dentist Patient C Patient A Patient G Patient G No other risk factors. Patient B All had invasive dental Patient E procedures. Patient A Dentist Local No2 Local No3 Sex partner with HIV Patient F Local No9 Local No35 Local No3 Behavioral risk for HIV Patient D Applications of phylogenetics: agriculture 1. What was the origin of a pest or agricultural disease species? 2. How did some pest organisms evolve resistance to pesticides? 3. How did a pest species spread through agriculture? 4. Are there species that are closely related to known pests that might also cause problems? 12
Applications of phylogenetics: agriculture (and health science) Fusarium : an economically significant fungal crop pathogen Powerful toxin that inhibits eukaryotic protein synthesis Applications of phylogenetics: agriculture Fursarium garminariam is a fungal pathogen of commercially important species of grains. Phylogenetic analysis indicates substantial genetic divergence among strains in different agricultural settings. Phylogenetic tree inferred from the combined gene sequences of six single-copy nuclear gene sequences (7,120 bp) by using the methods of maximum parsimony. Numbers above the nodes are bootstrap proportions. Genetic divergence among strains of Fusarium indicates that movement of crops among different agricultural settings must be carefully monitored to prevent introduction of “foreign strains”. Local crops are likely to be much less resistant to the “foreign” strains of Fusarium , as compared with the local strain. Figure adapted from O’Donnell et al. (2000) PNAS, 97:7905-7910. 13
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