THE CRCHUM’S CORE FACILITIES Biobanks Our new core facility since December 15, 2017 A dedicated floor at Montreal’s Hôtel-Dieu Hospital Pathology blocks, mainly cancer, at the present time Spaces for biobanks in -80 C freezers Spaces for liquid nitrogen tanks Secure environment, equipment maintained Alarm system with e-mail or phone notifications Assistance for sample and transportation management CL3 (Containment level 3): Controlled environment and access 8 culture rooms Cytometry – cell sorter Sample storage room
THE CRCHUM’S CORE FACILITIES Radiochemistry and cyclotron • Research & Development – Organic & Analytic Chemistry • Precursor & Standard synthesis – Radiochemistry – Biotesting & analysis of radioactive metabolites Services offered – Production (Carbon-11, Fluorine-18 and Nitrogen-13) – Existing radiotracers ([ 18 F]DCFPyL, [ 18 F]Choline, [ 18 F]HFB, [ 13 N]NH 4 Cl, [ 18 F]FTHA) – New radiotracers ([ 18 F]DMDA, [ 18 F]Fluoropyridine-Losartan, NEP derivates) – Quality control of PET radiotracers and organic molecules – Submission of Clinical Trial Applications (CTAs) to Health Canada
THE CRCHUM’S CORE FACILITIES Animal Facility: Environment with considerable expertise for successfully carrying out your research project with due regard to the animals and their welfare Transgenesis and animal modelling: Generation of transgenic mice and rats CRISPR/Cas/9 technology Rodent genotyping Stem cell microinjections Stem cell electroporation Stem cell karyotyping Embryo cryopreservation, thawing and transfer Experimental imaging: Animal and human 3T MRI CT-Angiography Ultrasound system MicroPET/CT
THE CRCHUM’S CORE FACILITIES Rodent Phenotyping Metabolics: Pancreatic islet isolation Insulin secretion on cell line, isolated pancreatic islets and in situ pancreas In vivo carbohydrate homeostasis In vivo energy metabolism Blood biochemistry Experimental models Cardiovascular: ART Optix MX2 small animal imaging Micro-CT scanner EchoMRI magnetic resonance imaging Rodent surgery and telemetry Metabolic cages Behavioural: Radial Arm Maze Elevated Plus Maze Morris Water Maze Novel Object Recognition and Open field testing devices Physioscan and DietMax Cages Instruments for measuring motor coordination and strength (RotaRod) CatWalk gait analysis Treadmill exercisers
THE CRCHUM’S CORE FACILITIES Metabolomics and cellular physiology: Targeted and quantitative MS analysis of soluble small-molecule metabolites and known lipids Discovery of new metabolites SeaHorse analyzer – O2 consumption and CO2 production Operetta – proliferation, apoptosis, migration, ROS, membrane potential, mitochondrial mass Luminex – Analysis of blood parameters by analyzer Quantification of analytes and hormones by ELISA and AlphaLIS Pharmacokinetics: In vitro metabolism (S9 fraction, microsomes, hepatocytes) Assessment of membrane transport Transfer, development, optimization and validation of methods PD bioanalysis (biomarkers) PK bioanalysis Surface plasmon resonance: Mesurement of different protein interactions (BiaCore 3000)
THE CRCHUM’S CORE FACILITIES Biostatistics: Writing funding applications and protocols Reviewing and critiquing various scientific documents (grants, articles, protocols) Project planning Advice on the direction of the project Calculation of sample size Data analysis Collaboration on publications Interpretation of results Personalized workshops (short seminar, facilitation of journal clubs, statistics workshops).
THE CRCHUM’S CORE FACILITIES Bioinformatics: Automation of analysis Support for the use of bioinformatics software Analysis of large datasets High-performance computing Database construction and maintenance Project consultation Web tool creation Scientific writing: Reviewing documents to identify weaknesses Reviewing to improve presentation, grammar and syntax Reviewing more thoroughly and recommending improvements in content Assistance in writing new documents
THE CRCHUM’S CORE FACILITIES UPCOMING ACTIVITIES EXPERIMENTAL IMAGING – January 25 Presentation on the areas of study and treatment that use magnetic stimulation MOLECULAR PATHOLOGY – February 1 Optimize your manipulations: immunohistochemistry, immunofluorescence, image processing and more CONFINEMENT LEVEL 3 – February 8 Presentation of the HIV-Primo and HIV-Aging cohorts RADIOCHEMISTRY AND CYCLOTRON – March 15 Translational studies that complement your research through the use of radioligands and PET imaging
THE CRCHUM’S CORE FACILITIES Website and registration http://crchum.chumontreal.qc.ca/plateformes-et-services Presentation of each core facility Contact information of technical and scientific managers Policies – procedures - pricing Check it out regularly! French and English versions Registration forms To fill out an application to use a facility or a service, contact the technical manager Payment authorization to be signed by the researcher COORDINATOR’S CONTACT INFORMATION Céline Coderre – ext. 30673 celine.coderre.chum@ssss.gouv.qc.ca
ANIMAL FACILITY Hélène Héon Veterinarian and Head of the Facility
ANIMAL FACILITY 38 members Animal health technicians Animal care providers Sterilization attendant Manager’s assistants Weekend staff Veterinarians
ANIMAL FACILITY TECHNICAL SERVICES Ensures animal maintenance, care and welfare Provides assistance for research projects Treatments Animal orders and import/export Hands-on training
ANIMAL FACILITY A NIMAL MODELING CORE FACILITY Embryo transfers Embryo and sperm cryopreservation In vitro fertilization Generation of transgenic or knock-out mice and rats (pronuclear microinjections, ES cells) CRISPR/Cas9 nucleases, Zinc Finger Nucleases (ZFNs) and Transcription Activator-Like Effector Nucleases (TALENs) Preparation of DNA for microinjections Preparation of ES cells Karyotyping CONTACT INFORMATION Jean ‐ François Schmouth, ext. 35224
ANIMAL FACILITY V ETERINARY DEPARTMENT Oversees the quality of care given to animals housed at the CRCHUM Provides project planning support (surgery, anaesthesia, animal care and supervision, endpoints, animal models, etc.) Animal care service CONTACT INFORMATION Hélène Héon – ext. 28867 Maryse Boulay – ext. 23719
ANIMAL FACILITY H OUSING AND SHARED PREMISES 13 th floor Rodent imaging Large animal surgery and imaging room Accommodations for large animals and fish Quarantine Culture room Instrument washing room Large animal necropsy room
ANIMAL FACILITY H OUSING AND SHARED PREMISES 14 th floor Rodent housing and manipulation room Animal modelling core facility Rodent surgery-telemetry-physiology room Equipment washing room (cages, bottles, etc.) Rodent necropsy room
ANIMAL FACILITY Mice Rats Rabbits Dogs Pigs Fish The animals are healthy; there is no danger of transmission of diseases to humans Some people may develop an allergy to rodents and rabbits
ANIMAL FACILITY Available to researchers, animal users and collaborators Hours of operation: 6:30 a.m.- 8:00 p.m. Housing rooms accessible from 6:30 a.m. - 6:30 p.m. Training required Programming of swipe cards depending on sectors covered CONTACT INFORMATION Cordélia Cadieux – ext. 28938
ANIMAL FACILITY DOCUMENTATION INTRANET CIPA ANIMAL FACILITY – CIPA USER TRAINING ANIMAL FACILITY HEALTH AND SAFETY Policies and procedures (animal facility and Biohazard committee o o CIPA) Radiation Protection Service o Department contact information o Hazardous material o Per diem list o Animal modelling core facility list o Forms o Helpful links o
INSTITUTIONAL ANIMAL PROTECTION COMMITTEE Christian Demers Coordinator Animal Protection Committee
INSTITUTIONAL ANIMAL PROTECTION COMMITTEE W HO IS CONCERNED BY THE CIPA ? All people who will be working on protocols using research animals. The CIPA is a committee that reviews protocols by assessing the expected benefits for the improvement of human or animal health and well-being.
INSTITUTIONAL ANIMAL PROTECTION COMMITTEE B ASIS FOR THE CIPA ’ S POLICIES The CCAC certifies our animal care and use program every three years on the basis of its compliance with the policies and guidelines of the CCAC and other standards recognized by the CCAC . POLICIES AND GUIDELINES OF THE CANADIAN COUNCIL ON ANIMAL CARE (CCAC) http://www.ccac.ca CONTACT INFORMATION Coordinator: Christian Demers – ext. 28947 Assistant: Geneviève Lauzon – ext. 24535 cipa.chum@ssss.gouv.qc.ca
INSTITUTIONAL ANIMAL PROTECTION COMMITTEE M ANDATORY TRAINING FOR ANIMAL USERS COMMON CORE LOCAL PROCEDURES/ORIENTATION/WELCOME TO THE ANIMAL FACILITIES LABORATORY PRACTICES (MICE, RATS, ASEPTIC SURGERY, ETC.)
INSTITUTIONAL ANIMAL PROTECTION COMMITTEE V OLUNTEERING After an information session, you can become a volunteer and come play with the dogs and cuddle them. If this interests you, please contact the CIPA or the Animal Facility.
LABORATORY SAFETY AND BIOHAZARDS Laurent Knafo Health and Safety Advisor CONTACT INFORMATION Laurent Knafo – ext. 26537 Laurent.knafo.chum@ssss.gouv.qc.ca
LABORATORY SAFETY AND BIOHAZARDS TRAINING EMERGENCY MEASURE PLAN GENERAL BIOSAFETY CONFINEMENT LABORATORIES
LABORATORY SAFETY AND BIOHAZARDS OTHER IMPORTANT TRAINING HAZARDOUS MATERIAL TRANSPORTATION WHMIS RADIATION PROTECTION
LABORATORY SAFETY AND BIOHAZARDS BIOHAZARDS MICROORGANISMS (VIRUSES, BACTERIA, ETC.) HUMAN ANATOMICAL SPECIMENS CELL CULTURES VIRAL VECTORS AND RECOMBINANT DNA TOXINS CYTOTOXIC DRUGS CHEMICALS
LABORATORY SAFETY AND BIOHAZARDS BIOSAFETY MANAGEMENT BIOSAFETY MANAGEMENT CRCHUM’s management team CRCHUM’s management team Institutional biosafety committee Institutional biosafety committee Biological safety agent Biological safety agent Students/Interns/Research assistants… Students/Interns/Research assistants… Federal and provincial agencies Federal and provincial agencies (PHAC/CFIA/Transport Canada/Environment (PHAC/CFIA/Transport Canada/Environment Canada) Canada)
LABORATORY SAFETY AND BIOHAZARDS Administrative controls Personal Engineering Biosafety protection controls equipment Practices and procedures
LABORATORY SAFETY AND BIOHAZARDS Policies in effect at the CRCHUM No: 90420 (dress code) No: 90460 (disciplinary measures)
LABORATORY SAFETY AND BIOHAZARDS IN CASE OF EMERGENCY 5 5 5 5 MANDATORY REPORTING OF INCIDENTS/SPILLS YOUR SAFETY = YOUR PRIORITY
THE CHUM LIBRARY Daniela Ziegler Librarian-Informationist
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