Using MAGIC to dissect the genetics of below- (and above-) ground - - PowerPoint PPT Presentation

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Aug 17, 2023 237 likes •332 views

Using MAGIC to dissect the genetics of below- (and above-) ground wheat phenotypes Yeorgia Argirou Specific aims Characterise wheat root (and shoot) architecture traits in the elite MAGIC mapping population Identify genetic loci that

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Using MAGIC to dissect the genetics of below- (and above-) ground wheat phenotypes

Yeorgia Argirou

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Characterise wheat root (and shoot) architecture

traits in the elite MAGIC mapping population

Identify genetic loci that control them
Design ‘breeder friendly’ genetic markers tagging

these loci

Specific aims

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Method and Phenotyping

Eight parents and 633 lines, grown in a lit and heated

glasshouse

4 reps of each parent and 1 or 2 reps of each line
4 seedlings per pot
Observed six traits:
Shoot height
Shoot area
Tillers
Root length
Dry root weight
Dry shoot weight

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GenStat analysis

Environmental variation on the bench:
Variation accounted for by using a mixed effects

model with spatial analysis

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RHT1

QTL mapping – Roots

Root length Root weight

Using R/mpMap and 90k (18k used) genotyped genetic map

(Gardner et al. 2015)

2 covariates used

Chr 4B Chr 1D

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QTL mapping - Shoots

Shoot area Shoot height Shoot weight Tillers

4B (RHT1) 4D (RHT2) 5A 5D 7B 5D 7D 7B 5D 5B 5A 2D

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Future work

Repeating the experiment
KASP marker development
Identify orthologues in rice

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