the Spanish provinces of Alicante and Valencia A. Yuste , F. - PowerPoint PPT Presentation

XII International Conference on Grapevine Breeding and Genetics (Bordeaux, July 15-20, 2018) Recovering ancient grapevines varieties in the Spanish provinces of Alicante and Valencia A. Yuste , F. Martínez-Gil, S. García-Martínez, D. López Lluch; R. Peiró R, C. Jiménez, J.J. Ruiz and C. Gisbert Other researchers collaborating to MINECO PROJECT CGL2015-70843-R reach this goal are from FEDER FUNDS

XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018 Comunit itat Vale lencia iana Alt de Benimaquia Denia Wine elaboration VII bc century BC Protected Geographical PROTECTED DESIGNATION Indication OF ORIGIN (PDO) Castellón

XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018 PROJECT CGL2015-70843-R  SSR profiles Identitity  Synonymies  Homonymies  Variability Germplams bank x  In vitro storage  Virus cleaning

XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018  New SSR profiles i.e.  Localization of varieties in risk of disapearance Planta Mula, Valencí negre, Botó de Gall, Arcos, Forcallat, Verdilet, Mamella de vaca, Raïm del clotet, Esclafagerres, etc.  Some of the synonymies found Grumer Moscatell Raïm del Clotet Esclafagerres Moscatel del terreno Moscatel d’ Alfàbega Muscat d’Istamboul (variety 17493 in VIVC) Gustico de Elche (Valencí blanc x Muscat of Alexandria; Moscatel Dulce Lacombe et al. 2013 ; Mena et al. 2014 ) Moscatel de Alicante Botó de Gall accessions Ahmeur bou Ahmeur (variety 140 in VIVC) Gisbert et al. 2018; Peiró et al. 2018; Jiménez et al. 2019

XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018  Analysis of variability ‘Moscatel de Alejandría’ ( Muscat of Alexandria) ‘ Monastrell ’ ( Mourvedre) GBS/46 accessions, 93.466 SNPs, Neighbour-Joining (NJ) bootstrap AFLPs/Peiró et al. 2018. Spanish Journal 1000. Reference clon MOU-369 of Agricultural Research vol.56(2)

Morenillo Carignan NI-5 Rojal Tinta Planta Nova-1 Planta Nova-2 Alicante Henry Bouchet Ullet de Perdiu Alcañon 53% chlorotype A Verdil-1 Verdil-2 Mari Sancho Trepadell 23% chlorotype D Planta Fina REF Planta Fina Alarije 13% chlorotype B Merseguera Bobal REF Bobal 11 % chlorotype C Planta Mula Morsí (Sbaa Tolba)- Macabeo Xarello REF Esclafagerres Tortosina Valencí Negre Valencí -Blanc-3 Valencí -Blanc-1 Valencí Blanc-2 NI-1 REF Bonicaire Forcallat Tinta Arcos Montalbana REF Benicarló Monastrell REF Monastrell Raïm del Clotet NI-3 NI-4 Inter- Morastell REF Pampolat Crujidera (Marufo) and REF Gateta Mondragón NI-2 Botó de Gall (Ahmeur bou Ahmeur) Intra- Mamella de Vaca Afus Ali-1 (Rosetti) Afus Ali-2 (Rosetti) varietal Alfonse Lavalleé Cardinal Cornichon Blanc variability Dominga Grumer Moscatell (Muscat d’Istanbul ) Muscat of Alexandria REF Moscatel E-110 Moscatel de Hamburgo Italia 0.05 SSRs/Jiménez et al. 2018/9

XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018  Analysis of variability ‘Moscatel de Alejandría’ ( Muscat of Alexandria) ‘ Monastrell ’ ( Mourvedre) 46 accesions, 93.466 SNPs, Neighbour-Joining (NJ) bootstrap Peiró et al. 2018. Spanish Journal of 1000. Reference clon MOU-369 Agricultural Research vol.56(2)

Morenillo Carignan NI-5 Rojal Tinta Planta Nova-1 Planta Nova-2 Alicante Henry Bouchet Ullet de Perdiu Alcañon 53% chlorotype A Verdil-1 Verdil-2 Mari Sancho 23% chlorotype D Trepadell Planta Fina 13% chlorotype B REF Planta Fina Alarije Merseguera 11 % chlorotype C Bobal REF Bobal Planta Mula Morsí (Sbaa Tolba)- Macabeo Xarello REF Esclafagerres Tortosina Valencí Negre Valencí -Blanc-3 Valencí -Blanc-1 Valencí Blanc-2 NI-1 REF Bonicaire Forcallat Tinta Arcos Montalbana REF Benicarló Monastrell REF Monastrell Raïm del Clotet NI-3 NI-4 Morastell REF Pampolat Crujidera (Marufo) Inter- REF Gateta Mondragón and NI-2 Botó de Gall (Ahmeur bou Ahmeur) Mamella de Vaca Intra- Afus Ali-1 (Rosetti) Afus Ali-2 (Rosetti) varietal Alfonse Lavalleé Cardinal Cornichon Blanc variability Dominga Grumer Moscatell (Muscat d’Istanbul ) Muscat of Alexandria REF Moscatel E-110 Moscatel de Hamburgo Italia 0.05 Jiménez et al. 2018/9

XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018  Virus analysis Grapevine fanleaf virus (GFLV) UE2002/11/CE High incidence Grapevine leafroll associated virus 1 (GLRaV-1) 35% simple Grapevine leafroll associated virus 3 (GLRaV-3) 37% doble Grapevine fleck virus (GFkV) 23% triple Arabis mosaic virus (ArMV) x  Development of protocols for virus cleaning Meristem culture Somatic embryogenesis

XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018  Development of protocols for in vitro storage

XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018 Thank you/Merci