the Spanish provinces of Alicante and Valencia A. Yuste , F. - - PowerPoint PPT Presentation

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the Spanish provinces of Alicante and Valencia A. Yuste , F. - - PowerPoint PPT Presentation

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XII International Conference on Grapevine Breeding and Genetics (Bordeaux, July 15-20, 2018) Recovering ancient grapevines varieties in the Spanish provinces of Alicante and Valencia A. Yuste , F. Martnez-Gil, S. Garca-Martnez, D. Lpez

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Recovering ancient grapevines varieties in the Spanish provinces of Alicante and Valencia

XII International Conference on Grapevine Breeding and Genetics (Bordeaux, July 15-20, 2018)

  • A. Yuste , F. Martínez-Gil, S. García-Martínez, D. López Lluch; R. Peiró

R, C. Jiménez, J.J. Ruiz and C. Gisbert

MINECO PROJECT CGL2015-70843-R FEDER FUNDS

Other researchers collaborating to reach this goal are from

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Comunit itat Vale lencia iana

PROTECTED DESIGNATION OF ORIGIN (PDO)

Alt de Benimaquia Denia

Wine elaboration VII bc century BC

XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018

Protected Geographical Indication Castellón

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  • Identitity
  • Synonymies
  • Homonymies
  • Variability

Germplams bank SSR profiles

  • In vitro storage
  • Virus cleaning

x

XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018

PROJECT CGL2015-70843-R

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Grumer Moscatell Moscatel del terreno Moscatel d’ Alfàbega Gustico de Elche Moscatel Dulce Moscatel de Alicante Muscat d’Istamboul (variety 17493 in VIVC) (Valencí blanc x Muscat of Alexandria; Lacombe et al. 2013 ; Mena et al. 2014) Gisbert et al. 2018; Peiró et al. 2018; Jiménez et al. 2019

Raïm del Clotet

 Some of the synonymies found Botó de Gall accessions Ahmeur bou Ahmeur (variety 140 in VIVC)

XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018

Esclafagerres

 New SSR profiles i.e.  Localization of varieties in risk of disapearance Planta Mula, Valencí negre, Botó de Gall, Arcos, Forcallat, Verdilet, Mamella de vaca, Raïm del clotet, Esclafagerres, etc.

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  • Analysis of variability

‘Monastrell’ (Mourvedre) ‘Moscatel de Alejandría’ (Muscat of Alexandria)

AFLPs/Peiró et al. 2018. Spanish Journal

  • f Agricultural Research vol.56(2)

XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018

GBS/46 accessions, 93.466 SNPs, Neighbour-Joining (NJ) bootstrap

  • 1000. Reference clon MOU-369
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Morenillo Carignan NI-5 Rojal Tinta Planta Nova-1 Planta Nova-2 Alicante Henry Bouchet Ullet de Perdiu Alcañon Verdil-1 Verdil-2 Mari Sancho Trepadell Planta Fina REF Planta Fina Alarije Merseguera Bobal REF Bobal Planta Mula Morsí (Sbaa Tolba)- Macabeo Xarello REF Esclafagerres Tortosina Valencí Negre Valencí -Blanc-3 Valencí -Blanc-1 Valencí Blanc-2 NI-1 REF Bonicaire Forcallat Tinta Arcos Montalbana REF Benicarló Monastrell REF Monastrell Raïm del Clotet NI-3 NI-4 Morastell REF Pampolat Crujidera (Marufo) REF Gateta Mondragón NI-2 Botó de Gall (Ahmeur bou Ahmeur) Mamella de Vaca Afus Ali-1 (Rosetti) Afus Ali-2 (Rosetti) Alfonse Lavalleé Cardinal Cornichon Blanc Dominga Grumer Moscatell (Muscat d’Istanbul) Muscat of Alexandria REF Moscatel E-110 Moscatel de Hamburgo Italia

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SSRs/Jiménez et al. 2018/9

53% chlorotype A 23% chlorotype D 13% chlorotype B 11 % chlorotype C Inter- and Intra- varietal variability

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  • Analysis of variability

‘Monastrell’ (Mourvedre) ‘Moscatel de Alejandría’ (Muscat of Alexandria)

Peiró et al. 2018. Spanish Journal of Agricultural Research vol.56(2)

XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018

46 accesions, 93.466 SNPs, Neighbour-Joining (NJ) bootstrap

  • 1000. Reference clon MOU-369
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Morenillo Carignan NI-5 Rojal Tinta Planta Nova-1 Planta Nova-2 Alicante Henry Bouchet Ullet de Perdiu Alcañon Verdil-1 Verdil-2 Mari Sancho Trepadell Planta Fina REF Planta Fina Alarije Merseguera Bobal REF Bobal Planta Mula Morsí (Sbaa Tolba)- Macabeo Xarello REF Esclafagerres Tortosina Valencí Negre Valencí -Blanc-3 Valencí -Blanc-1 Valencí Blanc-2 NI-1 REF Bonicaire Forcallat Tinta Arcos Montalbana REF Benicarló Monastrell REF Monastrell Raïm del Clotet NI-3 NI-4 Morastell REF Pampolat Crujidera (Marufo) REF Gateta Mondragón NI-2 Botó de Gall (Ahmeur bou Ahmeur) Mamella de Vaca Afus Ali-1 (Rosetti) Afus Ali-2 (Rosetti) Alfonse Lavalleé Cardinal Cornichon Blanc Dominga Grumer Moscatell (Muscat d’Istanbul) Muscat of Alexandria REF Moscatel E-110 Moscatel de Hamburgo Italia

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Jiménez et al. 2018/9 53% chlorotype A 23% chlorotype D 13% chlorotype B 11 % chlorotype C Inter- and Intra- varietal variability

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 Development of protocols for virus cleaning

 Virus analysis

UE2002/11/CE

XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018

Grapevine fanleaf virus (GFLV) Grapevine leafroll associated virus 1 (GLRaV-1) Grapevine leafroll associated virus 3 (GLRaV-3) Grapevine fleck virus (GFkV) Arabis mosaic virus (ArMV) x

Meristem culture Somatic embryogenesis High incidence 35% simple 37% doble 23% triple

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 Development of protocols for in vitro storage

XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018

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Thank you/Merci

XII International Conference on Grapevine Breeding and Genetics, Bordeaux, 2018