The science of bone marrow concentrate. Lisa A. Fortier, DVM, PhD, - - PowerPoint PPT Presentation
Cornell University College of Veterinary Medicine The science of bone marrow concentrate. Lisa A. Fortier, DVM, PhD, DACVS James Law Professor of Surgery laf4@cornell.edu Disclosures: Arthrex Consultant, VRN Consultant, Scientific Board
Disclosures: Arthrex Consultant, VRN Consultant, Scientific Board Kensey Nash
OJSM 2016
4x108
Eydt 2014 ? Heparin coated
No bony overgrowth Fill > 75% Fill < 25%
T2 mapping Axial FSE T1 rho T2 mapping Axial FSE T1 rho JBJS 2010 JBJS 2017
Williams Hematology
Bone marrow aspiration BMA BMC-A PRP WB BMC-B Cell Culture Colony forming units Flow cytometry:
Trilineage differentiation Cytology Cytokines Blood collection WB, PRP, BMA, BMC-A, BMC-B BMA, BMC-A, BMC-B Catabolic:
Leukocytes:
Platelets Anabolic:
Cassano KSSTA 2016 N=29 48.2 yrs (23-68) Courtesy, Kennedy
Mean ± S.D. PRP BMC p-value WBC thou/ul 3.1 ± 1.2 36.7 ± 16.9 <0.0001 Neutrophils thou/ul 0.9 ± 0.4 17.5 ± 9.8 0.0002 Monocytes thou/ul 0.3 ± 0.2 3.3 ± 2.3 0.0005 Platelets thou/uL 202.3 ± 86.1 151.6 ± 111.2 0.46
Mean ± S.D. PRP BMC p-value PDGF pg/mL 5975 ± 2644 7549 ± 3601 0.25 TGF-β2 pg/mL 65± 70 219 ± 219 0.024 VEGF pg/mL 10 ± 19 276 ± 273 0.0005 IL-8 pg/mL < 3.50 273 ± 300 N/A IL-1β pg/mL < 0.70 7 ± 13 N/A
Not detectable: IL-6, TNF-α, INF-γ, FGF
In horses since 2007!