The NIH Human Microbiome Project: An Update Eric Green, M.D., Ph.D. - - PowerPoint PPT Presentation

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Aug 09, 2023 192 likes •320 views

The NIH Human Microbiome Project: An Update Eric Green, M.D., Ph.D. Director, NHGRI The NIH Human Microbiome Project (HMP) (HMPI: 2008-2012) (Canada) Sequencing Centers Demonstration Projects Data Analysis & Coordination Center

SLIDE 1

The NIH Human Microbiome Project: An Update

Eric Green, M.D., Ph.D.

Director, NHGRI

SLIDE 2

The NIH Human Microbiome Project (HMP)

(HMPI: 2008-2012)

(Canada)

Sequencing Centers Demonstration Projects Data Analysis & Coordination Center Technology Development Computational Tools ELSI Clinical labs

SLIDE 3

Demonstration Projects

Skin: eczema, psoriasis GI: Crohn’s disease, esophageal adenocarcinoma, necrotizing enterocolitis, pediatric IBS, ulcerative colitis Urogenital: bacterial vaginosis, circumcision, sexual histories Healthy cohort study Clinically healthy 300 male/female 18-40 y.o. 18 body sites Up to 3 visits in 2 yrs 16S rDNA, WGS metagenomes

Community resources

Repositories:

sequence data
microbiome
human
strains
clinical/phenotype data
nucleic acid extracts
cell lines

6 Initiatives in HMP

Interact through DACC and 400+ member consortium IHMC founding member

www.hmpdacc.org

1st Phase of the Human Microbiome Project (HMP1)

HMP Consortium

HMP1: Survey of the microbiome in humans

 Taxonomic analysis of microbial communities living in/on human body  Analysis of collective genetic potential in these microbial genomes (metagenomics)

SLIDE 4

In adults, each part of the body supports a distinct microbial community

No apparent relationship with gender, age, weight, ethnicity, or race

PCoA2 (4.4%) PCoA1 (13%) All taxon levels

SLIDE 5

Gevers et al., PLoS (2012)

HMP1 suggests that microbiomes in healthy U.S. adults reflect a global pool of:

~ 10,000 microbial species ~ 8 million unique microbial genes

SLIDE 6

doi:10.1038/nature 1209 doi:10.1038/nature11209 doi/10.1371/issue.pcol.v01.i13

Key HMP1 Publications (open access)

doi:10.1038/nature11234 doi/10.1371/issue.pcol.v01.i13 20+ companion papers

SLIDE 7

HMP1 Publications and Their Impact

20 40 60 80 100 120 140 160 10 20 30 40 50 60 70

2009 2010 2011 2012 HMP-Supported Publications Citations per Publication

Median Citations per Publication Number of Publications

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HMP1-Related Media Inquiries: 2007-2013

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What are the 8 million microbial genes doing?

DNA RNA Proteins Metabolism

SLIDE 10

HMP2 (2013-2015):

1) Dataset of microbiome & host genomic and biological properties 2) Community resource 3) Up to $5M/yr, up to 2 awards

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http://commonfund.nih.gov/hmp Where to go for more information about HMP?

Also – visit NIH Common Fund site for current list of 320+ HMP papers: http://commonfund.nih.gov/hmp/publications.aspx